Abstract:
:Differential scanning calorimetry and CD spectrometry were employed to study the thermal unfolding of light meromyosin (LMM) prepared from carp acclimated to different temperatures. The transition temperatures given by the major peaks at pH 8.0 in 0.6 M KCl for LMM from carp acclimated to 10 degrees C were 32.5 and 39.5 degrees C with the calorimetric enthalpies (DeltaHcal) of 269 and 52 kcal/mol, respectively. LMM from carp acclimated to 20 degrees C exhibited three peaks of transition temperatures at 34.5, 40.2, and 46.9 with DeltaHcal of 152, 20, and 10 kcal/mol, respectively. On the other hand, LMM from carp acclimated to 30 degrees C showed two different patterns. The first experiment gave two transition temperatures at 39.2 and 47.3 degrees C with DeltaHcal of 231 and 39 kcal/mol, respectively. The second series of experiments resulted in showing three peaks of 34.4, 39.5, and 47.5 degrees C with DeltaHcal of 117, 123, and 28 kcal/mol, respectively. N-terminal amino acid sequence analysis revealed that LMM at the second series of experiments with the 30 degrees C-acclimated carp contained component(s) predominant in the 20 degrees C-acclimated carp. Thermal unfolding responsible for these transition temperatures was well explained by melting of alpha-helices which could be determined by far-ultraviolet CD spectroscopy. These results clearly demonstrate that the 30 degrees C-acclimated carp contained the most thermostable LMM.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nakaya M,Kakinuma M,Watabe S,Ooi Tdoi
10.1021/bi9701181subject
Has Abstractpub_date
1997-07-29 00:00:00pages
9179-84issue
30eissn
0006-2960issn
1520-4995pii
bi9701181journal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::RNA:pseudouridine synthetase (Pus1) from Saccharomyces cerevisiae is a multisite specific enzyme that catalyzes the formation of pseudouridine at positions 34 and 36 of intron-containing precursor tRNAIle and at positions 27 and/or 28 of several yeast tRNAs. In this paper we demonstrate that the purified recombinant P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972671o
更新日期:1998-05-19 00:00:00
abstract::Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011595t
更新日期:2001-12-25 00:00:00
abstract::The malaria parasite pigment, hemozoin, is a crystal of ferriprotoporphyrin IX (FP-Fe(III)), a product of hemoglobin digestion. Hemozoin formation is essential for FP-Fe(III) detoxification in the parasite; it is the main target of quinoline antimalarials and can modulate immune and inflammation responses. To gain fur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100567j
更新日期:2010-08-10 00:00:00
abstract::Site-directed mutagenesis and random mutagenesis were used to produce variants of subtilisin BPN' (Bacillus amyloliquefaciens) protease with variable surface adsorption properties. Protease adsorption and peptide hydrolysis rate were measured for these variants using a model substrate consisting of a peptide covalentl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951990h
更新日期:1996-03-12 00:00:00
abstract::The crystal structure of the ternary complex citrate synthase-oxaloacetate-carboxymethyl coenzyme A has been solved to a resolution of 1.9 A and refined to a conventional crystallographic R factor of 0.185. The structure resembles a proposed transition state of the condensation reaction and suggests that the condensat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-03-06 00:00:00
abstract::We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a024
更新日期:1984-12-18 00:00:00
abstract::Lysophosphatidic acid (1-acyl-sn-glycero-3-phosphate or LPA) is a phospholipid mediator displaying numerous and widespread biological activities and thought to act via G-protein-coupled receptors. Here we have studied the effects on human platelets of a number of LPA analogues, including two enantiomers of both N-palm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9816756
更新日期:1999-06-29 00:00:00
abstract::The c-Jun N-terminal kinases (JNKs) play a wide variety of roles in cellular signaling processes, dictating important, and even divergent, cellular fates. These essential kinases possess docking surfaces distal to their active sites that interact with diverse binding partners, including upstream activators, downstream...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00776
更新日期:2018-10-09 00:00:00
abstract::The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00231a022
更新日期:1991-04-30 00:00:00
abstract::We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00440a050
更新日期:1989-07-11 00:00:00
abstract::The ability of a class of C-20' modified vinca alkaloid congeners to induce tubulin spiral formation was investigated relative to their ability to inhibit microtubule assembly, their cytotoxicity against a leukemic cell line, L1210, and their measured and calculated partition coefficients. These studies were prompted ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001038r
更新日期:2000-10-03 00:00:00
abstract::The interaction of serine protease (esterases) with 6-chloro-2-pyrones was investigated. Time-dependent inactivation of chymotrypsin, alpha-lytic protease, pig liver elastase, and cholinesterase was found with 3- and 5-benzyl-6-chloro-2-pyrone, as well as 3- and 5-methyl-6-chloro-2-pyrone. No inactivation was observed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00282a034
更新日期:1983-06-21 00:00:00
abstract::The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960401k
更新日期:1996-07-23 00:00:00
abstract::The chirality of sulfoxidation catalyzed by two cytochrome P-450 isozymes purified from phenobarbital-induced rat liver was studied by using 4-tolyl ethyl sulfide as a substrate. Both P-450 isoenzymes, termed PB-1 and PB-4, when reconstituted with purified rat liver NADPH-cytochrome P-450 reductase and cytochrome b5, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00539a032
更新日期:1982-05-11 00:00:00
abstract::RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020319r
更新日期:2002-09-10 00:00:00
abstract::The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1015056
更新日期:2010-11-30 00:00:00
abstract::The high-affinity interaction between urokinase-type plasminogen activator (uPA) and its glycolipid anchored receptor (uPAR) is essential for the confinement of plasminogen activation to cell surfaces where it is thought to play an important role in cancer cell invasion and metastasis. The receptor binding site of uPA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00039a006
更新日期:1995-10-03 00:00:00
abstract::We have detected at least four forms of DNA-dependent ATPase in mouse FM3A cell extracts [Tawaragi, Y., Enomoto, T., Watanabe, Y., Hanaoka, F., & Yamada, M. (1984) Biochemistry 23, 529-533]. The purified fraction of one of the four forms, ATPase B, has been shown to have DNA helicase activity by using a DNA substrate ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00384a038
更新日期:1987-05-19 00:00:00
abstract::The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1014503
更新日期:2010-12-07 00:00:00
abstract::Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801202a
更新日期:2008-10-07 00:00:00
abstract::Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034394t
更新日期:2003-06-24 00:00:00
abstract::Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101462h
更新日期:2010-11-30 00:00:00
abstract::Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800286m
更新日期:2008-05-13 00:00:00
abstract::The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9521351
更新日期:1996-02-27 00:00:00
abstract::The kinetics of binding of chicken cystatin to papain were studied by stopped-flow fluorometry under pseudo-first-order conditions, i.e., with an excess of inhibitor. All reactions showed first-order behavior, and the observed pseudo-first-order rate constant increased linearly with the cystatin concentration up to th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00430a022
更新日期:1989-02-21 00:00:00
abstract::A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a023
更新日期:1977-05-03 00:00:00
abstract::The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a001
更新日期:1994-05-31 00:00:00
abstract::The mechanism of the slow refolding reactions of four different pancreatic ribonucleases from ox, sheep, red deer, and roe deer has been investigated. Refolding kinetics of these proteins were very similar. In particular, a native-like intermediate, IN, was shown to be populated on the slow refolding pathway of all ri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a005
更新日期:1985-07-16 00:00:00
abstract::Mouse fibroblasts contain a macromolecular binding component (receptor) which binds glucocorticoids specifically and with high affinity. This study shows that there are three different cellular forms of bound receptor and that it is experimentally possible to markedly alter the subcellular distribution of these three ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00672a030
更新日期:1975-01-14 00:00:00
abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485068
更新日期:2004-11-30 00:00:00