当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > DNA-dependent adenosinetriphosphatase B from mouse FM3A cells has DNA helicase activity.

DNA-dependent adenosinetriphosphatase B from mouse FM3A cells has DNA helicase activity.

Abstract：

:We have detected at least four forms of DNA-dependent ATPase in mouse FM3A cell extracts [Tawaragi, Y., Enomoto, T., Watanabe, Y., Hanaoka, F., & Yamada, M. (1984) Biochemistry 23, 529-533]. The purified fraction of one of the four forms, ATPase B, has been shown to have DNA helicase activity by using a DNA substrate which permits the detection of limited unwinding of the helix. The DNA substrate consists of single-stranded circular fd DNA and the hexadecamer complementary to the fd DNA, which bears an oligo(dT) tail at the 3' terminus. The helicase activity and DNA-dependent ATPase activity cosedimented at 5.5 S on glycerol gradient centrifugation. The helicase required a divalent cation for activity (Mg2+ congruent to Mn2+ greater than Ca2+). The optimal concentrations of these divalent cations were 5 mM. The requirement of divalent cations of the DNA helicase activity was very similar to that for the DNA-dependent ATPase activity of ATPase B. The helicase activity was absolutely dependent on the presence of a nucleoside triphosphate. ATP was the most effective cofactor among the ribo- and deoxyribonucleoside triphosphates tested, and considerable levels of helicase activity were observed with other ribo- and deoxyribonucleoside triphosphates. The efficiency of a nucleoside triphosphate to serve as cofactor for the helicase activity correlated with the capacity of the nucleotide to serve as substrate for the DNA-dependent ATPase activity. The nonhydrolyzable ATP analogues such as adenosine 5'-O-(3-thiotriphosphate) were not effective for the helicase activity.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Seki M,Enomoto T,Hanaoka F,Yamada M

doi

10.1021/bi00384a038

subject

Has Abstract

pub_date

1987-05-19 00:00:00

pages

2924-8

issue

10

eissn

0006-2960

issn

1520-4995

journal_volume

26

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Triacylglycerol and phospholipid hydrolysis in human plasma lipoproteins: role of lipoprotein and hepatic lipase.

    abstract：:To explore the interactions of triacylglycerol and phospholipid hydrolysis in lipoprotein conversions and remodeling, we compared the activities of lipoprotein and hepatic lipases on human VLDL, IDL, LDL, and HDL2. Triacylglycerol and phospholipid hydrolysis by each enzyme were measured concomitantly in each lipoprote...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00151a023

    authors： Deckelbaum RJ,Ramakrishnan R,Eisenberg S,Olivecrona T,Bengtsson-Olivecrona G

    更新日期：1992-09-15 00:00:00

  • A structure-based approach to a synthetic vaccine for HIV-1.

    abstract：:The generation of neutralizing antibodies by peptide immunization is dependent on achieving conformational compatibility between antibodies and native protein. Consequently, approaches are needed for developing conformational mimics of protein neutralization sites. We replace putative main-chain hydrogen bonds (NH -->...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0003691

    authors： Cabezas E,Wang M,Parren PW,Stanfield RL,Satterthwait AC

    更新日期：2000-11-28 00:00:00

  • Mechanism of inhibition of the (Ca2(+)-Mg2+)-ATPase by nonylphenol.

    abstract：:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00464a028

    authors： Michelangeli F,Orlowski S,Champeil P,East JM,Lee AG

    更新日期：1990-03-27 00:00:00

  • Phospholamban binds in a compact and ordered conformation to the Ca-ATPase.

    abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035424v

    authors： Li J,Xiong Y,Bigelow DJ,Squier TC

    更新日期：2004-01-20 00:00:00

  • The orientation and accessibility of substrates on the active site of triosephosphate isomerase.

    abstract：:Tritiated sodium borohydride was used to reduce the substrates of triosephosphate isomerase in the presence of the enzyme, and the mixture of the four possible products (D-[1(R)-3H]; D-[1(S)-3H]-; D-[2-3H]-, and L-[2-3H]glycerol 3-phosphate) was analyzed. While enzyme-bound dihydroxyacetone phosphate is reduced comple...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00692a020

    authors： Webb MR,Knowles JR

    更新日期：1975-10-21 00:00:00

  • The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.

    abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00047a003

    authors： Barak LS,Ménard L,Ferguson SS,Colapietro AM,Caron MG

    更新日期：1995-11-28 00:00:00

  • Location of the antigenic determinants of bovine pancreatic ribonuclease.

    abstract：:Four antigenic regions of native bovine pancreatic ribonuclease have been located by using antibodies that react specifically with segments 1--13, 31--79, and 80--124. These antibodies were purified by affinity chromatography on columns to which these peptide segments were bound. Analysis of precipitin curves indicate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00587a019

    authors： Chavez LG Jr,Scheraga HA

    更新日期：1979-10-02 00:00:00

  • Solution structure of the first Sam domain of Odin and binding studies with the EphA2 receptor.

    abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300141h

    authors： Mercurio FA,Marasco D,Pirone L,Pedone EM,Pellecchia M,Leone M

    更新日期：2012-03-13 00:00:00

  • Stabilization of the second oxyanion intermediate by 1,4-dihydroxy-2-naphthoyl-coenzyme A synthase of the menaquinone pathway: spectroscopic evidence of the involvement of a conserved aspartic acid.

    abstract：:1,4-Dihydroxy-2-naphthoyl-coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes an intramolecular Claisen condensation involving two oxyanion intermediates in the biosynthetic pathway of menaquinone, an essential respiration electron transporter in many microorganisms. Here we report the finding that the DHNA-CoA product...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200376x

    authors： Chen M,Jiang M,Sun Y,Guo ZF,Guo Z

    更新日期：2011-07-05 00:00:00

  • Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin.

    abstract：:Annexin A1 is a multifunctional, calcium-dependent phospholipid binding protein involved in a host of processes including inflammation, regulation of neuroendocrine signaling, apoptosis, and membrane trafficking. Binding of annexin A1 to glycans has been implicated in cell attachment and modulation of annexin A1 funct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101121a

    authors： Horlacher T,Noti C,de Paz JL,Bindschädler P,Hecht ML,Smith DF,Fukuda MN,Seeberger PH

    更新日期：2011-04-05 00:00:00

  • Two-step purification of the major phosphorylated protein in reticulocyte 40S ribosomal subunits.

    abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00604a003

    authors： Du Vernay VH Jr,Traugh JA

    更新日期：1978-05-30 00:00:00

  • Structure of an RNA hairpin loop with a 5'-CGUUUCG-3' loop motif by heteronuclear NMR spectroscopy and distance geometry.

    abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971207g

    authors： Sich C,Ohlenschläger O,Ramachandran R,Görlach M,Brown LR

    更新日期：1997-11-18 00:00:00

  • Spectroscopic Determination of Distinct Heme Ligands in Outer-Membrane Receptors PhuR and HasR of Pseudomonas aeruginosa.

    abstract：:Pseudomonas aeruginosa PAO1 encodes two outer membrane receptors, PhuR (Pseudomonas heme uptake) and HasR (heme assimilation system). The HasR receptor acquires heme through interaction with a secreted hemophore, HasAp. The non-hemophore-dependent PhuR is encoded along with proteins required for heme translocation int...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00017

    authors： Smith AD,Modi AR,Sun S,Dawson JH,Wilks A

    更新日期：2015-04-28 00:00:00

  • The divalent cation is obligatory for the binding of ligands to the catalytic site of secreted phospholipase A2.

    abstract：:The divalent cation requirement for partial reactions of the catalytic turnover cycle during interfacial catalysis by pig pancreatic phospholipase A2 (PLA2) is investigated. Results show that the specific role of calcium in all the events of the catalytic cycle at the active site is not shared by other divalent cation...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00076a024

    authors： Yu BZ,Berg OG,Jain MK

    更新日期：1993-06-29 00:00:00

  • Evidence for targeted gene delivery to Hep G2 hepatoma cells in vitro.

    abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00403a008

    authors： Wu GY,Wu CH

    更新日期：1988-02-09 00:00:00

  • Interactions of poly(N epsilon, N epsilon, N epsilon-trimethyllysine) and poly(N delta, N delta, N delta-trimethylornithine) with polynucleotides: salt dissociation and thermal denaturation.

    abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00555a020

    authors： Granados EN,Bello J

    更新日期：1980-07-08 00:00:00

  • The novel bovine serpin endopin 2C demonstrates selective inhibition of the cysteine protease cathepsin L compared to the serine protease elastase, in cross-class inhibition.

    abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050053z

    authors： Hwang SR,Stoka V,Turk V,Hook VY

    更新日期：2005-05-31 00:00:00

  • Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site.

    abstract：:Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0341814

    authors： Gao X,Wen X,Esser L,Quinn B,Yu L,Yu CA,Xia D

    更新日期：2003-08-05 00:00:00

  • Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics.

    abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960482k

    authors： Mchaourab HS,Lietzow MA,Hideg K,Hubbell WL

    更新日期：1996-06-18 00:00:00

  • Adding fingers to an engineered zinc finger nuclease can reduce activity.

    abstract：:Zinc finger nucleases (ZFNs) have been used to direct precise modifications of the genetic information in living cells at high efficiency. An important consideration in the design of ZFNs is the number of zinc fingers that are required for efficient and specific cleavage. We examined dimeric ZFNs composed of [1]+[1], ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200393g

    authors： Shimizu Y,Şöllü C,Meckler JF,Adriaenssens A,Zykovich A,Cathomen T,Segal DJ

    更新日期：2011-06-07 00:00:00

  • Affinity labeling of Escherichia coli phenylalanyl-tRNA synthetase at the binding site for tRNAPhe.

    abstract：:Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00391a033

    authors： Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

    更新日期：1987-08-25 00:00:00

  • Three-dimensional solution structure of an immunoglobulin light chain-binding domain of protein L. Comparison with the IgG-binding domains of protein G.

    abstract：:Protein L is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species Peptostreptococcus magnus. It has affinity for immunoglobulin (Ig) through interaction with framework structures in the variable Ig light chain domain. The Ig-binding activity is located to five homologous re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00251a008

    authors： Wikström M,Drakenberg T,Forsén S,Sjöbring U,Björck L

    更新日期：1994-11-29 00:00:00

  • Calorimetric study of the interaction of lysozyme with aqueous 1-propanol.

    abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00514a016

    authors： Sturtevant JM,Velicelebi G

    更新日期：1981-05-26 00:00:00

  • Human SAS-6 C-Terminus Nucleates and Promotes Microtubule Assembly in Vitro by Binding to Microtubules.

    abstract：:Centrioles are essential components of the animal centrosome and play crucial roles in the formation of cilia and flagella. They are cylindrical structures composed of nine triplet microtubules organized around a central cartwheel. Recent studies have identified spindle assembly abnormal protein SAS-6 as a critical co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00978

    authors： Gupta H,Badarudeen B,George A,Thomas GE,Gireesh KK,Manna TK

    更新日期：2015-10-20 00:00:00

  • A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis.

    abstract：:Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resist...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971276v

    authors： Madhusudan M,Zapf J,Hoch JA,Whiteley JM,Xuong NH,Varughese KI

    更新日期：1997-10-21 00:00:00

  • Dynamics and binding mode of Hoechst 33258 to d(GTGGAATTCCAC)2 in the 1:1 solution complex as determined by two-dimensional 1H NMR.

    abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00112a004

    authors： Fede A,Labhardt A,Bannwarth W,Leupin W

    更新日期：1991-12-03 00:00:00

  • Effect of alpha-methylation on inactivation of monoamine oxidase by N-cyclopropylbenzylamine.

    abstract：:Monoamine oxidase (MAO) was shown previously [Silverman, R. B., & Hoffman, S. J. (1980) J. Am. Chem. Soc. 102, 7126-7128] to catalyze the oxidation of N-cyclopropylbenzylamine (N-CBA) at two sites on the molecule. Oxidation at the benzyl methylene gave benzaldehyde and cyclopropylamine; oxidation of the cyclopropyl gr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00317a019

    authors： Silverman RB

    更新日期：1984-10-23 00:00:00

  • Kinetic mechanism for the flipping and excision of 1,N(6)-ethenoadenine by AlkA.

    abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501356x

    authors： Taylor EL,O'Brien PJ

    更新日期：2015-01-27 00:00:00

  • Refinement of the solution structure of the DNA dodecamer 5'd(CGCGPATTCGCG)2 containing a stable purine-thymine base pair: combined use of nuclear magnetic resonance and restrained molecular dynamics.

    abstract：:The solution structure of the self-complementary dodecamer 5'd(CGCGPATTCGCG)2, containing a purine-thymine base pair within the hexameric canonical recognition site GAATTC for the restriction endonuclease EcoRI, is investigated by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Nonexchangeab...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00411a042

    authors： Clore GM,Oschkinat H,McLaughlin LW,Benseler F,Happ CS,Happ E,Gronenborn AM

    更新日期：1988-05-31 00:00:00

  • Protected sulfur transfer reactions by the Escherichia coli Suf system.

    abstract：:The first step in sulfur mobilization for the biosynthesis of Fe-S clusters under oxidative stress and iron starvation in Escherichia coli involves a cysteine desulfurase SufS. Its catalytic reactivity is dependent on the presence of a sulfur acceptor protein, SufE, which acts as the preferred substrate for this enzym...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4001479

    authors： Selbach BP,Pradhan PK,Dos Santos PC

    更新日期：2013-06-11 00:00:00