Spectroscopic Determination of Distinct Heme Ligands in Outer-Membrane Receptors PhuR and HasR of Pseudomonas aeruginosa.

abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...

journal_title：Biochemistry

authors： Addington AK,Johnson DA

更新日期：1996-10-22 00:00:00

abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...

journal_title：Biochemistry

authors： Klein C,Hollender J,Bender H,Schulz GE

更新日期：1992-09-22 00:00:00

abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

journal_title：Biochemistry

authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

更新日期：2001-04-17 00:00:00

abstract：:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...

journal_title：Biochemistry

authors： Maurizi MR,Ginsburg A

更新日期：1986-01-14 00:00:00

abstract：:Assembly of the type I procollagen molecule begins with interactions among the C-pro alpha1(I) and C-pro alpha2(I) domains. The C-propeptide domains themselves have subdomains of distinct structures. The important questions are where chain association begins and the basis of the chain selectivity which leads to the pr...

journal_title：Biochemistry

authors： Malone JP,Alvares K,Veis A

更新日期：2005-11-22 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...

journal_title：Biochemistry

authors： Molle V,Kremer L,Girard-Blanc C,Besra GS,Cozzone AJ,Prost JF

更新日期：2003-12-30 00:00:00

abstract：:In previous work from our laboratory, 5-10% of the channels formed by [Val1]gramicidin A have conductances that fall outside the narrow range that conventionally has defined the standard gramicidin channel [e.g., see Russell et al. (1986) Biophys. J. 49, 673]. Reports from other laboratories, however, show that up to ...

journal_title：Biochemistry

authors： Sawyer DB,Koeppe RE 2nd,Andersen OS

更新日期：1989-08-08 00:00:00

abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

journal_title：Biochemistry

authors： Kaur H,Scaria V,Maiti S

更新日期：2010-11-09 00:00:00

abstract：:We studied the binding of peptides containing five basic residues to membranes containing acidic lipids. The peptides have five arginine or lysine residues and zero, one, or two alanines between the basic groups. The vesicles were formed from mixtures of a zwitterionic lipid, phosphatidylcholine, and an acidic lipid, ...

journal_title：Biochemistry

authors： Mosior M,McLaughlin S

更新日期：1992-02-18 00:00:00

abstract：:The diazomethyl ketones of z-Phe-Phe inactivate papain by a stoichiometric reaction at the active-center thiol. Since the reagents are stable in mercaptoethanol, their reaction with papain is judged to be the result of complex formation characteristic of affinity-labeling reagents. The diazomethyl ketones react by a m...

journal_title：Biochemistry

authors： Leary R,Larsen D,Watanabe H,Shaw E

更新日期：1977-12-27 00:00:00

abstract：:A conformational analysis of the valine side chains of ribonuclease T1 (RNase T1) was performed using NMR spectroscopy, in particular homonuclear (1H, 1H and 13C, 13C) and heteronuclear (1H, 15N and 1H, 13C) vicinal spin-spin coupling constants as obtained from E.COSY-type NMR experiments. The coupling constants relat...

journal_title：Biochemistry

authors： Karimi-Nejad Y,Schmidt JM,Rüterjans H,Schwalbe H,Greisinger C

更新日期：1994-05-10 00:00:00

abstract：:The aminolysis of the depsipeptide m-[[(phenylacetyl)glycyl]oxy]benzoic acid (1) by D-phenylalanine, catalyzed by the beta-lactamase of Enterobacter cloacae P99, is inhibited by the product of the reaction, (phenylacetyl)glycyl-D-phenylalanine (2), by the peptide analogue of 1, m-[(phenylacetyl)-glycinamido]benzoic ac...

journal_title：Biochemistry

authors： Pazhanisamy S,Pratt RF

更新日期：1989-08-22 00:00:00

abstract：:The mechanisms for substrate recognition by two cytoplasmic protein tyrosine phosphatases, PTP-5 and rrbPTP-1, were investigated. Phosphorylation sites on tyrosine-phosphorylated casein, a model PTP substrate, were characterized. Two peptides based on casein phosphorylation sites and one peptide based on the tyrosine ...

journal_title：Biochemistry

authors： Hippen KL,Jakes S,Richards J,Jena BP,Beck BL,Tabatabai LB,Ingebritsen TS

更新日期：1993-11-23 00:00:00

abstract：:DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...

journal_title：Biochemistry

authors： Jacobs KA,Schlessinger D

更新日期：1977-03-08 00:00:00

abstract：:13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...

journal_title：Biochemistry

authors： Gerken TA

更新日期：1984-09-25 00:00:00

abstract：:Phosphite dehydrogenase (PTDH) is a unique NAD-dependent enzyme that catalyzes the oxidation of inorganic phosphite to phosphate. The enzyme has great potential for cofactor regeneration, and mechanistic studies have provided some insight into the residues that are important for catalysis. In this investigation, pre-s...

journal_title：Biochemistry

authors： Fogle EJ,van der Donk WA

更新日期：2007-11-13 00:00:00

abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...

journal_title：Biochemistry

authors： Jha S,Snell JM,Sheftic SR,Patil SM,Daniels SB,Kolling FW,Alexandrescu AT

更新日期：2014-01-21 00:00:00

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:The pH dependence of the transient aerobic kinetics of cytochromes c and a has been investigated with cytochrome oxidase reconstituted in phospholipid vesicles in the absence and presence of an uncoupler and an ionophore. The cytochrome a reduction level immediately after the burst phase was 60-80% and was not signifi...

journal_title：Biochemistry

authors： Maison-Peteri B,Malmström BG

更新日期：1989-04-18 00:00:00

abstract：:The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...

journal_title：Biochemistry

authors： deCastro MJ,Ho CH,Stewart RJ

更新日期：1999-04-20 00:00:00

abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...

journal_title：Biochemistry

authors： Gulbinas V,Karpicz R,Garab G,Valkunas L

更新日期：2006-08-08 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) (E. C. 3.1.1.4) is a common enzyme in the two-way cascade mechanism leading to the production of proinflammatory compounds known as eicosanoids. The binding of phospholipase A(2) to the membrane surface and hydrolysis of phospholipids are thought to involve the formation of a hydrophobic ch...

journal_title：Biochemistry

authors： Singh RK,Vikram P,Makker J,Jabeen T,Sharma S,Dey S,Kaur P,Srinivasan A,Singh TP

更新日期：2003-10-14 00:00:00

abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...

journal_title：Biochemistry

authors： Yang K,Strauss E,Huerta C,Zhang H

更新日期：2008-02-05 00:00:00

abstract：:A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...

journal_title：Biochemistry

authors： Branlant G,Eiler B,Biellmann JF,Lutz HP,Luisi PL

更新日期：1983-09-13 00:00:00

abstract：:Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...

journal_title：Biochemistry

authors： Borowy-Borowski H,Lipman R,Chowdary D,Tomasz M

更新日期：1990-03-27 00:00:00

abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...

journal_title：Biochemistry

authors： Chen T,Haigentz M Jr,Reisler E

更新日期：1992-03-24 00:00:00

abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...

journal_title：Biochemistry

authors： Pappas A,Park TS,Carman GM

更新日期：1999-12-14 00:00:00

abstract：:4-Hydroxybenzoyl-coenzyme A (4-HBA-CoA) thioesterase catalyzes the hydrolysis of 4-HBA-CoA to 4-hydroxybenzoate and CoA. X-ray crystallographic analysis of the liganded enzyme has shown that the benzoyl thioester and pantetheine moieties of the substrate ligand are bound in a narrow crevice while the nucleotide moiety...

journal_title：Biochemistry

authors： Zhuang Z,Song F,Zhang W,Taylor K,Archambault A,Dunaway-Mariano D,Dong J,Carey PR

更新日期：2002-09-17 00:00:00