Affinity labeling of Escherichia coli phenylalanyl-tRNA synthetase at the binding site for tRNAPhe.

abstract：:MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from...

journal_title：Biochemistry

authors： Bean GJ,Amann KJ

更新日期：2008-01-15 00:00:00

abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...

journal_title：Biochemistry

authors： Christensen T,Svensson B,Sigurskjold BW

更新日期：1999-05-11 00:00:00

abstract：:To gain insight into the mechanism by which Arg-163 influences oligomerization of alphaA-crystallin, we prepared a series of truncated alphaA-crystallins with or without mutation of the Arg-163 residue. Expression of the proteins was achieved in Escherichia coli BL21 (DE3) pLysS cells, and alphaA-crystallin was purifi...

journal_title：Biochemistry

authors： Rajan S,Chandrashekar R,Aziz A,Abraham EC

更新日期：2006-12-26 00:00:00

abstract：:Upon blue light irradiation, photoactive yellow protein (PYP) undergoes a conformational change that involves large movements at the N-terminus of the protein. We reasoned that this conformational change might be used to control other protein or peptide sequences if these were introduced as linkers connecting the N- a...

journal_title：Biochemistry

authors： Kumar A,Burns DC,Al-Abdul-Wahid MS,Woolley GA

更新日期：2013-05-14 00:00:00

abstract：:The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...

journal_title：Biochemistry

authors： Pucci P,Siciliano R,Malorni A,Marino G,Tecce MF,Ceccarini C,Terrana B

更新日期：1991-05-21 00:00:00

abstract：:Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococc...

journal_title：Biochemistry

authors： Majsnerowska M,Ter Beek J,Stanek WK,Duurkens RH,Slotboom DJ

更新日期：2015-08-11 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00

abstract：:31P NMR spectra of equilibrium mixtures of enzyme-bound reactants and products of the adenylate kinase reaction (formula; see text) were analyzed by using computer simulations based on density matrix theory of chemical exchange. Since adenylate kinase has the unique feature that the reactants in the reverse direction ...

journal_title：Biochemistry

authors： Vasavada KV,Kaplan JI,Nageswara Rao BD

更新日期：1984-02-28 00:00:00

abstract：:The specific effect of the binding of IF2 and initiator fMET-tRNA(fMet) on Escherichia coli 16S rRNA has been probed by phosphate alkylation with ethylnitrosourea. The results show that IF2 does not significantly shield portions of 16S rRNA but induces both reductions and enhancements of reactivity scattered in the en...

journal_title：Biochemistry

authors： Wakao H,Romby P,Laalami S,Ebel JP,Ehresmann C,Ehresmann B

更新日期：1990-09-04 00:00:00

abstract：:We have examined the ability of the Escherichia coli single-stranded DNA binding protein (SSB) tetramer to form its different binding modes on poly(dC), poly(U), and poly(A) over a range of NaCl and NaF concentrations for comparison with previous studies with poly(dT). In reverse titrations with poly(U) and poly(A) at...

journal_title：Biochemistry

authors： Lohman TM,Bujalowski W

更新日期：1994-05-24 00:00:00

abstract：:Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...

journal_title：Biochemistry

authors： Wang H,Seeman NC

更新日期：1995-01-24 00:00:00

abstract：:Extended illumination slows the rebinding of CO to myoglobin after photodissociation at cryogenic temperatures. Two types of models have been put forward to explain the effect: motions of the CO within the heme pocket or conformational transitions of the protein. To resolve this ambiguity, we have studied the effect o...

journal_title：Biochemistry

authors： Nienhaus GU,Mourant JR,Chu K,Frauenfelder H

更新日期：1994-11-15 00:00:00

abstract：:The ligation state of hemoglobin during its cross-linking by glycolaldehyde influences the ultimate oxygen affinity of the cross-linked protein. Thus, if the cross-linking is performed with carbonmonoxy-hemoglobin, the oxygen affinity increases slightly to a P50 of 7 mmHg from a P50 of 9 mmHg for unmodified hemoglobin...

journal_title：Biochemistry

authors： Manning LR,Manning JM

更新日期：1988-08-23 00:00:00

abstract：:The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...

journal_title：Biochemistry

authors： Herold S,Exner M,Nauser T

更新日期：2001-03-20 00:00:00

abstract：:Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is com...

journal_title：Biochemistry

authors： Andel F 3rd,Lagarias JC,Mathies RA

更新日期：1996-12-17 00:00:00

abstract：:Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...

journal_title：Biochemistry

authors： Bloom JW,Nesheim ME,Mann KG

更新日期：1979-10-02 00:00:00

abstract：:We studied hyperosmolarity-induced changes in cell volume and cytoplasmic pH in PS120 cells expressing Na(+)/H(+) exchanger (NHE) isoforms and their mutants. Change in cell volume was estimated by measuring change in cell height by means of confocal microscopy. Regulatory volume increase (RVI) and cytoplasmic alkalini...

journal_title：Biochemistry

authors： Su X,Pang T,Wakabayashi S,Shigekawa M

更新日期：2003-02-04 00:00:00

abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

journal_title：Biochemistry

authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

更新日期：2001-12-18 00:00:00

abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

journal_title：Biochemistry

authors： Cloherty EK,Levine KB,Carruthers A

更新日期：2001-12-25 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...

journal_title：Biochemistry

authors： Zhang Y,Wavreille AS,Kunys AR,Pei D

更新日期：2009-11-24 00:00:00

abstract：:Uroporphyrinogen III synthase (U3S) catalyzes the asymmetrical cyclization of a linear tetrapyrrole to form the physiologically relevant uroporphyrinogen III (uro'gen III) isomer during heme biosynthesis. Here, we report four apoenzyme and one product complex crystal structures of the Thermus thermophilus (HB27) U3S p...

journal_title：Biochemistry

authors： Schubert HL,Phillips JD,Heroux A,Hill CP

更新日期：2008-08-19 00:00:00

abstract：:Mammalian phospholipase D (PLD) has been implicated in the cellular signal transduction pathways leading to diverse physiological events and known to be regulated by many cellular factors. To identify the proteins that interact with PLD, we performed a protein overlay assay with fractions obtained from the sequential ...

journal_title：Biochemistry

authors： Kim JH,Lee S,Kim JH,Lee TG,Hirata M,Suh PG,Ryu SH

更新日期：2002-03-12 00:00:00

abstract：:In this work, we have studied the role of the arginine finger region in determining the specificity of the GTPase activating proteins (GAPs) Saccharomyces cerevisiae Ira2p and human p120-GAP toward yeast Ras2p and human Ha-Ras p21. It is known that p120-GAP can enhance both Ras2p and Ha-Ras GTPase activities, whereas ...

journal_title：Biochemistry

authors： te Biesebeke R,Krab IM,Parmeggiani A

更新日期：2001-06-26 00:00:00

abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

journal_title：Biochemistry

authors： Roychowdhury S,Rasenick MM

更新日期：1994-08-16 00:00:00

abstract：:The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel. These are associated to homotrimers which are unusually stable, due mostly to hydrophobic interactions between the beta-barrels. In addition, a loop, ...

journal_title：Biochemistry

authors： Phale PS,Philippsen A,Kiefhaber T,Koebnik R,Phale VP,Schirmer T,Rosenbusch JP

更新日期：1998-11-10 00:00:00

abstract：:Full-length protein tyrosine phosphatase 1C (PTP1C), the catalytic domain of PTP1C (delta PTP1C), and the N-terminal SH2 domain truncated PTP1C (delta NPTP1C) were overexpressed in Escherichia coli and purified to near homogeneity. Various phosphorylated states of the synthetic phosphotyrosyl peptide TRDIYETDYYRK (IRP...

journal_title：Biochemistry

authors： Townley R,Shen SH,Banville D,Ramachandran C

更新日期：1993-12-14 00:00:00

abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...

journal_title：Biochemistry

authors： Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

更新日期：1998-04-28 00:00:00

abstract：:Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...

journal_title：Biochemistry

authors： Cosman M,Fiala R,Hingerty BE,Laryea A,Lee H,Harvey RG,Amin S,Geacintov NE,Broyde S,Patel D

更新日期：1993-11-23 00:00:00

abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...

journal_title：Biochemistry

authors： Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez B

更新日期：2008-01-08 00:00:00

abstract：:The mitochondrial folate transporter (MFT) was previously identified in human and hamster cells. Sequence homology of this protein with the inner mitochondrial membrane transporters suggested a domain structure in which the N- and C-termini of the protein are located on the mitochondrial intermembrane-facing surface, ...

journal_title：Biochemistry

authors： Perchiniak E,Lawrence SA,Kasten S,Woodard BA,Taylor SM,Moran RG

更新日期：2007-02-13 00:00:00