A conformational rearrangement in gramicidin A: from a double-stranded left-handed to a single-stranded right-handed helix.

abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

journal_title：Biochemistry

authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

更新日期：1981-07-07 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are ubiquitous in all three kingdoms of life and are implicated in many clinically relevant physiological processes. They couple the energy released by ATP hydrolysis to facilitate substrate translocation across cell membranes. The crystal structures of type II ABC importers hav...

journal_title：Biochemistry

authors： Pan C,Weng J,Wang W

更新日期：2016-12-13 00:00:00

abstract：:Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell-cell adhesion by homophilic interactions. To reveal the molecular details of calcium binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting of the first two domains of E-cadherin (EC...

journal_title：Biochemistry

authors： Koch AW,Pokutta S,Lustig A,Engel J

更新日期：1997-06-24 00:00:00

abstract：:The organization principles underlying non-membrane-bound organelles have started to unravel in the past 10 years. A new biophysical model known as biomolecular condensates has been proposed to explain many aspects of membraneless organelle assembly and regulation. Neurons are extremely complex, and each neuron can co...

journal_title：Biochemistry

authors： Feng Z,Zeng M,Chen X,Zhang M

更新日期：2018-05-01 00:00:00

abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...

journal_title：Biochemistry

authors： Kliegman JI,Griner SL,Helmann JD,Brennan RG,Glasfeld A

更新日期：2006-03-21 00:00:00

abstract：:The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici has been solved using X-ray diffraction data extending to atomic resolution, 0.94 A, recorded at 100 K. The model was refined with anisotropic representation of atomic displacement parameters for all non-hydrogen atoms and with hydrogens ridi...

journal_title：Biochemistry

authors： Dauter Z,Wilson KS,Sieker LC,Meyer J,Moulis JM

更新日期：1997-12-23 00:00:00

abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...

journal_title：Biochemistry

authors： Watson J,Nilsen-Hamilton M,Hamilton RT

更新日期：1976-04-06 00:00:00

abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

journal_title：Biochemistry

authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

更新日期：2002-11-05 00:00:00

abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

journal_title：Biochemistry

authors： Chen FM

更新日期：1984-12-04 00:00:00

abstract：:Following platelet activation, platelets undergo a dramatic shape change mediated by the actin cytoskeleton and accompanied by secretion of granule contents. While the actin cytoskeleton is thought to influence platelet granule secretion, the mechanism for this putative regulation is not known. We found that disruptio...

journal_title：Biochemistry

authors： Woronowicz K,Dilks JR,Rozenvayn N,Dowal L,Blair PS,Peters CG,Woronowicz L,Flaumenhaft R

更新日期：2010-06-01 00:00:00

abstract：:(E)-4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB) catalyzes the terminal step of the MEP/DOXP pathway where it converts (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into the two products, isopentenyl diphosphate and dimethylallyl diphosphate. The reaction involves the reductive elimination ...

journal_title：Biochemistry

authors： Xu W,Lees NS,Hall D,Welideniya D,Hoffman BM,Duin EC

更新日期：2012-06-19 00:00:00

abstract：:It has been inferred from structural and computational studies that the mechanism of DNA polymerases involves subtle but important discrete steps that occur between binding and recognition of the correct dNTP and chemical catalysis. These steps potentially include local conformational changes involving active site res...

journal_title：Biochemistry

authors： Balbo PB,Wang EC,Tsai MD

更新日期：2011-11-15 00:00:00

abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...

journal_title：Biochemistry

authors： Fewtrell C,Kempner E,Poy G,Metzger H

更新日期：1981-11-10 00:00:00

abstract：:The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...

journal_title：Biochemistry

authors： Gao X,Brown FK,Jeffs P,Bischofberger N,Lin KY,Pipe AJ,Noble SA

更新日期：1992-07-14 00:00:00

abstract：:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...

journal_title：Biochemistry

authors： Kresheck GC,Adade AB,Vanderkooi G

更新日期：1985-03-26 00:00:00

abstract：:Terminase enzymes are responsible for "packaging" of viral DNA into a preformed procapsid. Bacteriophage lambda terminase is composed of two subunits, gpA and gpNu1, in a gpA(1).gpNu1(2) holoenzyme complex. The larger gpA subunit is responsible for preparation of viral DNA for packaging, and is central to the packagin...

journal_title：Biochemistry

authors： Gaussier H,Ortega ME,Maluf NK,Catalano CE

更新日期：2005-07-19 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:Cysteine dioxygenase (CDO) is a non-heme monoiron enzyme with an unusual posttranslational modification in the proximity of the ferrous iron active site. This modification, a cysteine to tyrosine thioether bond, cross-links two β-strands of the β-barrel. We have investigated its role in catalysis through a combined cr...

journal_title：Biochemistry

authors： Davies CG,Fellner M,Tchesnokov EP,Wilbanks SM,Jameson GN

更新日期：2014-12-23 00:00:00

abstract：:Deletion of Glu146, Thr147, and Trp148 from thrombin dramatically alters its interactions with substrates, ligands, and inhibitors, and the changes appear to result from nonlocal modification of thrombin's structure [Le Bonniec, B. F., Guinto, E. R., & Esmon, C. T. (1992) J. Biol. Chem. 267, 19341-19348]. In an attemp...

journal_title：Biochemistry

authors： Le Bonniec BF,Betz A,Guinto ER,Esmon CT,Stone SR

更新日期：1994-04-05 00:00:00

abstract：:To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quen...

journal_title：Biochemistry

authors： Asuncion-Punzalan E,Kachel K,London E

更新日期：1998-03-31 00:00:00

abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...

journal_title：Biochemistry

authors： Ghosh D,Sahay S,Ranjan P,Salot S,Mohite GM,Singh PK,Dwivedi S,Carvalho E,Banerjee R,Kumar A,Maji SK

更新日期：2014-10-21 00:00:00

abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...

journal_title：Biochemistry

authors： Santoro MM,Liu Y,Khan SM,Hou LX,Bolen DW

更新日期：1992-06-16 00:00:00

abstract：:Four murine myeloma immunoglobulins, A4, A47N, U61, and E109, have been studied for their binding affinities with inulin and a series of oligosaccharides derived from inulin. The results indicate that the combining site of these immunoglobulins shows highest complementarity for a trifructofuranosyl sequence (A4 and A4...

journal_title：Biochemistry

authors： Streefkerk DG,Glaudemans CP

更新日期：1977-08-23 00:00:00

abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...

journal_title：Biochemistry

authors： Huo X,Viola RE

更新日期：1996-12-17 00:00:00

abstract：:The association between the papillomavirus E1 and E2 proteins is an important regulatory interaction, imparting coordinated control of viral transcription and replication. Using fluorescence polarization, we have characterized the interactions between HPV-11 E1, HPV-11 E2, and DNA in solution at equilibrium. For these...

journal_title：Biochemistry

authors： Chao SF,Rocque WJ,Daniel S,Czyzyk LE,Phelps WC,Alexander KA

更新日期：1999-04-06 00:00:00

abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...

journal_title：Biochemistry

authors： House CH,Miller SL

更新日期：1996-01-09 00:00:00

abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

journal_title：Biochemistry

authors： Carter GJ,van Holde K

更新日期：1998-09-08 00:00:00

abstract：:Human telomeres are comprised of d(TTAGGG) repeats that are capable of forming G-quadruplex DNA structures. Ligands that bind to and stabilize these G-quadruplex DNA structures are potential inhibitors of the cancer cell-associated enzyme telomerase. Other potential biological uses of G-quadruplex targeting ligands ha...

journal_title：Biochemistry

authors： Kern JT,Thomas PW,Kerwin SM

更新日期：2002-09-24 00:00:00

abstract：:The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...

journal_title：Biochemistry

authors： Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

更新日期：1980-09-16 00:00:00

abstract：:Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...

journal_title：Biochemistry

authors： Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

更新日期：1997-12-16 00:00:00