Binding studies on anti-fructofuranan mouse myeloma immunoglobulins A47N, A4, U61, and E109.

abstract：:Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

journal_title：Biochemistry

authors： Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

更新日期：1999-03-02 00:00:00

abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

journal_title：Biochemistry

authors： Flock U,Watmough NJ,Adelroth P

更新日期：2005-08-09 00:00:00

abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...

journal_title：Biochemistry

authors： Ilsley DD,Lee SH,Miller WH,Kuchta RD

更新日期：1995-02-28 00:00:00

abstract：:The role of the protein shell in the formation of the hydrous ferric oxide core of ferritin is poorly understood. A VO2+ spin probe study was undertaken to characterize the initial complex of Fe2+ with horse spleen apoferritin (96% L-subunits). A competitive binding study of VO2+ and Fe2+ showed that the two metals co...

journal_title：Biochemistry

authors： Hanna PM,Chasteen ND,Rottman GA,Aisen P

更新日期：1991-09-24 00:00:00

abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...

journal_title：Biochemistry

authors： Huo X,Viola RE

更新日期：1996-12-17 00:00:00

abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...

journal_title：Biochemistry

authors： Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

更新日期：1987-08-11 00:00:00

abstract：:The disulfide structure of sillucin, a highly knotted, cysteine-rich, antimicrobial peptide, isolated from Rhizomucor pusillus, has been determined to be Cys2--Cys7, Cys12--Cys24, Cys13--Cys30, and Cys14--Cys21 by disulfide mass mapping based on partial reduction and CN-induced cleavage enabled by cyanylation. The den...

journal_title：Biochemistry

authors： Qi J,Wu J,Somkuti GA,Watson JT

更新日期：2001-04-17 00:00:00

abstract：:The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...

journal_title：Biochemistry

authors： Vasil'ev S,Bruce D

更新日期：2000-11-21 00:00:00

abstract：:In our previous work [Yang, Z. W., & Babitch, J. A. (1988) Biochemistry (preceding paper in this issue)] divalent cations were found to be more effective promoters of astroglial filament formation than were monovalent cations. To determine if one or more divalent cation binding sites were the basis for this difference...

journal_title：Biochemistry

authors： Yang ZW,Kong CF,Babitch JA

更新日期：1988-09-06 00:00:00

abstract：:The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...

journal_title：Biochemistry

authors： Hoyle NP,O'Neill JS

更新日期：2015-01-20 00:00:00

abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...

journal_title：Biochemistry

authors： Marinetti TD,Snyder GH,Sykes BD

更新日期：1977-02-22 00:00:00

abstract：:Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...

journal_title：Biochemistry

authors： Vunnam N,Szymonski S,Hirsova P,Gores GJ,Sachs JN,Hackel BJ

更新日期：2020-10-13 00:00:00

abstract：:A new procedure for the synthesis of the pyrophosphate bond has been employed in the preparation of nucleoside dipyrophosphates from nucleoside 3',5'-diphosphates. The method makes use of a powerful phosphorylating agent generated in a mixture of cyanoethyl phosphate, dicyclohexylcarbodiimide, and mesitylenesulfonyl c...

journal_title：Biochemistry

authors： Bennett GN,Gough GR,Gilham PT

更新日期：1976-10-19 00:00:00

abstract：:We studied hyperosmolarity-induced changes in cell volume and cytoplasmic pH in PS120 cells expressing Na(+)/H(+) exchanger (NHE) isoforms and their mutants. Change in cell volume was estimated by measuring change in cell height by means of confocal microscopy. Regulatory volume increase (RVI) and cytoplasmic alkalini...

journal_title：Biochemistry

authors： Su X,Pang T,Wakabayashi S,Shigekawa M

更新日期：2003-02-04 00:00:00

abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...

journal_title：Biochemistry

authors： Green KD,Biswas T,Pang AH,Willby MJ,Reed MS,Stuchlik O,Pohl J,Posey JE,Tsodikov OV,Garneau-Tsodikova S

更新日期：2018-02-06 00:00:00

abstract：:UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...

journal_title：Biochemistry

authors： Myles GM,Hearst JE,Sancar A

更新日期：1991-04-23 00:00:00

abstract：:Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...

journal_title：Biochemistry

authors： Muranyi A,Finn BE,Gippert GP,Forsén S,Stenflo J,Drakenberg T

更新日期：1998-07-28 00:00:00

abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...

journal_title：Biochemistry

authors： Rutherford TJ,Neville DC,Homans SW

更新日期：1995-10-31 00:00:00

abstract：:Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...

journal_title：Biochemistry

authors： Shiozawa JA,Jelenska MM,Jacobson BS

更新日期：1987-07-28 00:00:00

abstract：:Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...

journal_title：Biochemistry

authors： Swedberg JE,Harris JM

更新日期：2011-10-04 00:00:00

abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...

journal_title：Biochemistry

authors： Shaw GS,Sykes BD

更新日期：1996-06-11 00:00:00

abstract：:A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...

journal_title：Biochemistry

authors： Dubois J,Chapman SK,Mathews FS,Reid GA,Lederer F

更新日期：1990-07-10 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...

journal_title：Biochemistry

authors： Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

更新日期：2010-11-02 00:00:00

abstract：:The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...

journal_title：Biochemistry

authors： Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

更新日期：2002-06-18 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...

journal_title：Biochemistry

authors： Ruan Z,Kannan N

更新日期：2015-07-14 00:00:00

abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

journal_title：Biochemistry

authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

更新日期：2013-02-26 00:00:00

abstract：:We have undertaken ultrafast infrared (IR) spectroscopic studies in order to elucidate the geometry of bound CO in the alpha and beta subunits of hemoglobin (Hb) M Boston 13CO. Hb M Boston is a mutant human Hb in which the distal histidine in the alpha subunits is replaced by a tyrosine. The IR absorptions of bound 13...

journal_title：Biochemistry

authors： Lian T,Locke B,Kitagawa T,Nagai M,Hochstrasser RM

更新日期：1993-06-08 00:00:00

abstract：:Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...

journal_title：Biochemistry

authors： Wang Y,Kendall J,Cavet JS,Giedroc DP

更新日期：2010-08-10 00:00:00

abstract：:The serotonin transporter (SERT) is a member of a highly homologous family of proteins responsible for the reuptake of biogenic amines from the synaptic cleft. We took advantage of native restriction sites in SERT to construct a chimeric transporter containing a small (34 amino acid) region of the norepinephrine trans...

journal_title：Biochemistry

authors： Stephan MM,Chen MA,Penado KM,Rudnick G

更新日期：1997-02-11 00:00:00