Important role of the proline residue in the signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae.

abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...

journal_title：Biochemistry

authors： Naito Y,Lowenstein JM

更新日期：1981-09-01 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ...

journal_title：Biochemistry

authors： Ray WJ Jr,Long JW

更新日期：1976-09-07 00:00:00

abstract：:The propeptide binding/activation site on the vitamin K dependent carboxylase has been localized to a region of carboxylase between residues Arg +50 and Glu +225 by photoinactivation studies using [125I]tyrosyl-labeled benzoylphenylalanine (Bpa)-containing analogs of proFIX19, a peptide containing residues -18 to +1 o...

journal_title：Biochemistry

authors： Yamada M,Kuliopulos A,Nelson NP,Roth DA,Furie B,Furie BC,Walsh CT

更新日期：1995-01-17 00:00:00

abstract：:The cofactor product of the aromatic amino acid hydroxylases, 4a-hydroxy-6(R)-tetrahydrobiopterin, requires dehydration before tetrahydrobiopterin can be regenerated by dihydropteridine reductase. Carbinolamine dehydration occurs nonenzymatically, but the reaction is also catalyzed by 4a-hydroxytetrahydropterin dehydr...

journal_title：Biochemistry

authors： Rebrin I,Bailey SW,Boerth SR,Ardell MD,Ayling JE

更新日期：1995-05-02 00:00:00

abstract：:Out of over 60 single-Cys mutants in putative periplasmic loops in lactose permease, three mutants [Tyr101 --> Cys (loop III/IV), Leu313 --> Cys (loop IX/X), and Ser375 --> Cys (loop XI/XII)] spontaneously form disulfide-linked dimers, indicating that these loops are located on the periphery of the 12-helix bundle tha...

journal_title：Biochemistry

authors： Sun J,Kaback HR

更新日期：1997-09-30 00:00:00

abstract：:In the ras gene superfamily, codon 12 (-TGGTG-) of the K-ras gene is the most frequently mutated codon in human cancers. Recently, we have found that bulky chemical carcinogens preferentially form DNA adducts at codons 12 and 14 (-CGTAG-) in the K-ras gene in normal human bronchial epithelial (NHBE) cells. Furthermore...

journal_title：Biochemistry

authors： Hu W,Feng Z,Tang MS

更新日期：2003-08-26 00:00:00

abstract：:Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...

journal_title：Biochemistry

authors： Lowe PN,Beechey RB

更新日期：1982-08-17 00:00:00

abstract：:Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...

journal_title：Biochemistry

authors： Van Houten B,Illenye S,Qu Y,Farrell N

更新日期：1993-11-09 00:00:00

abstract：:The solution structure and dynamics of human insulin are investigated by 2D 1H NMR spectroscopy in reference to a previously analyzed analogue, des-pentapeptide(B26-B30) insulin (DPI; Hua, Q.X., & Weiss, M.A. (1990) Biochemistry 29, 10545-10555). This spectroscopic comparison is of interest since (i) the structure of ...

journal_title：Biochemistry

authors： Hua QX,Weiss MA

更新日期：1991-06-04 00:00:00

abstract：:The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...

journal_title：Biochemistry

authors： Choi KA,Yang L,Lee KM,Yu CW,Banfield DK,Ito K,Uchiumi T,Wong KB

更新日期：2019-09-10 00:00:00

abstract：:Sumoylation, the process by which the ubiquitin-related SUMO protein is covalently attached to lysine side chains in other proteins, is involved in numerous processes in the eukaryotic cell, including transcriptional repression. In this study, we identify Gcn5, the histone-modifying subunit of the transcriptional regu...

journal_title：Biochemistry

authors： Sterner DE,Nathan D,Reindle A,Johnson ES,Berger SL

更新日期：2006-01-24 00:00:00

abstract：:The effects of urea on apomyoglobin solubility have been investigated. Apomyoglobin precipitation was found to be a thermodynamically reversible process independent of the pathway of aggregation. A liquid-solid phase diagram was constructed for the precipitation of apomyoglobin as a function of urea and protein concen...

journal_title：Biochemistry

authors： De Young LR,Dill KA,Fink AL

更新日期：1993-04-20 00:00:00

abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

journal_title：Biochemistry

authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

更新日期：2008-08-05 00:00:00

abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...

journal_title：Biochemistry

authors： Martinez KL,Corringer PJ,Edelstein SJ,Changeux JP,Mérola F

更新日期：2000-06-13 00:00:00

abstract：:In polyglutamine (polyQ) containing fragments of the Huntington's disease protein huntingtin (htt), the N-terminal 17 amino acid htt(NT) segment serves as the core of α-helical oligomers whose reversible assembly locally concentrates the polyQ segments, thereby facilitating polyQ amyloid nucleation. A variety of aggre...

journal_title：Biochemistry

authors： Jayaraman M,Mishra R,Kodali R,Thakur AK,Koharudin LM,Gronenborn AM,Wetzel R

更新日期：2012-04-03 00:00:00

abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...

journal_title：Biochemistry

authors： Isom HC,DeMoss RD

更新日期：1975-09-23 00:00:00

abstract：:The structure of the vanadate-trapped ADP complex of a truncated head of Dictyostelium myosin II consisting of residues Asp 2-Asn 762 has been determined by molecular replacement at 1.9 A resolution and refined to a crystallographic R-factor of 19.4%. The crystals belong to the orthorhombic space group C2221 where a =...

journal_title：Biochemistry

authors： Smith CA,Rayment I

更新日期：1996-04-30 00:00:00

abstract：:Inflammation and subsequent cyclooxygenase-2 (COX-2) activity has long been linked with the development of cancer, although little is known about any epigenetic effects of COX-2. A product of COX-2 activation, levuglandin (LG) quickly forms covalent bonds with nearby primary amines, such as those in lysine, which lead...

journal_title：Biochemistry

authors： Carrier EJ,Zagol-Ikapitte I,Amarnath V,Boutaud O,Oates JA

更新日期：2014-04-22 00:00:00

abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...

journal_title：Biochemistry

authors： Petri V,Hsieh M,Jamison E,Brenowitz M

更新日期：1998-11-10 00:00:00

abstract：:We have investigated the microsecond rotational motions of the Ca-ATPase in rabbit skeletal sarcoplasmic reticulum (SR), by measuring the time-resolved phosphorescence anisotropy of erythrosin 5-isothiocyanate (ERITC) covalently and specifically attached to the enzyme. Over a wide range of solvent conditions and tempe...

journal_title：Biochemistry

authors： Birmachu W,Thomas DD

更新日期：1990-04-24 00:00:00

abstract：:Helicases unwind double-stranded nucleic acids, remove secondary structures from single-stranded nucleic acids, and remove proteins bound to nucleic acids. For many helicases, the mechanisms for these different functions share the ability to translocate with a directional bias as a result of ATP binding and hydrolysis...

journal_title：Biochemistry

authors： Reynolds KA,Cameron CE,Raney KD

更新日期：2015-07-14 00:00:00

abstract：:[125I]Calmodulin binding to isolated rat adipocyte plasma membranes has been characterized, and the calmodulin binding proteins associated with the membrane have been identified by use of the photoaffinity cross-linker N-hydroxysuccinimidyl 4-azidobenzoate. Total binding of [125I]calmodulin to plasma membranes was ass...

journal_title：Biochemistry

authors： Goewert RR,Landt M,McDonald JM

更新日期：1982-10-12 00:00:00

abstract：:The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...

journal_title：Biochemistry

authors： Hoyle NP,O'Neill JS

更新日期：2015-01-20 00:00:00

abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...

journal_title：Biochemistry

authors： Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R

更新日期：2008-02-19 00:00:00

abstract：:In order to study in detail the generality of the NUX (N = A, U, G, or C; X = A, U, or C) rule for the GUC triplet adjacent to the cleavage site in hammerhead ribozymes, two kinetic parameters, namely, kcat and Km, were determined for substrates with mutations in this triplet, which included double mutants with mutati...

journal_title：Biochemistry

authors： Shimayama T,Nishikawa S,Taira K

更新日期：1995-03-21 00:00:00

abstract：:Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...

journal_title：Biochemistry

authors： Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

更新日期：2011-02-01 00:00:00

abstract：:There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...

journal_title：Biochemistry

authors： Nagano S,Tanaka M,Ishimori K,Watanabe Y,Morishima I

更新日期：1996-11-12 00:00:00

abstract：:NADPH-cytochrome P450 oxidoreductase (P450 reductase, EC 1.6.2.4) is an essential component of the P450 monooxygenase complex and binds FMN, FAD, and NADPH cofactors. Residues Tyr140 and Tyr178 are known to be involved in FMN binding. A third aromatic side chain, Phe181, is also located in the proximity of the FMN rin...

journal_title：Biochemistry

authors： Paine MJ,Ayivor S,Munro A,Tsan P,Lian LY,Roberts GC,Wolf CR

更新日期：2001-11-13 00:00:00

abstract：:Bacteriophage P22 is a double-stranded DNA containing phage. Its morphogenetic pathway requires the formation of a precursor procapsid that subsequently matures to the capsid. The stability of bacteriophage P22 coat protein in both monomeric and polymeric forms under hydrostatic pressure has been examined previously [...

journal_title：Biochemistry

authors： Foguel D,Teschke CM,Prevelige PE Jr,Silva JL

更新日期：1995-01-31 00:00:00