Abstract:
:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native enzyme with dimethylpimelimidate followed by gel electrophoresis shows that the enzyme is a dimer. The enzyme hydrolyzes all nucleoside 5'-monophosphates tested. A comparison of Vmax/Km for 14 different substrates shows that AMP is the best substrate. The enzyme shows lowest Km values for AMPS, AMP, isoAMP, GMP, and IMP. It shows no activity with nucleoside 2'- and 3'-monophosphates, sugar phosphates, and p-nitrophenyl phosphate, even when tested at high enzyme concentrations. The optimum activity of the enzyme occurs at pH 7.5 with AMP as substrate. Above this pH, buffer ions affect the activity in a complex manner, a second optimum being observed under some conditions. Magnesium ions activate the enzyme above pH 7.5 in the presence of some buffer ions but not of others. Magnesium ions show only a slight activation when the reaction is run in diethanolamine buffer, pH 9.5, at 30 degrees C; the activation in this buffer is considerably greater when the reaction is run at 37 degrees C. The enzyme is strongly inhibited by free ADP, maximum inhibition occurring below pH 6. The ADP inhibition is diminished as the pH is raised above 6, becoming negligible above pH9. The enzyme is inhibited by EDTA. The inhibition is partially reversed when the EDTA is removed from the enzyme by gel filtration. This as well as other evidence indicates that the enzyme contains a tightly bound metal ion.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Naito Y,Lowenstein JMdoi
10.1021/bi00521a014subject
Has Abstractpub_date
1981-09-01 00:00:00pages
5188-94issue
18eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::An antibody population that reacted with the high-affinity lysine binding site of human plasminogen was elicited by immunizing rabbits with an elastase degradation product containing kringles 1-3 (EDP I). This antibody was immunopurified by affinity chromatography on plasminogen-Sepharose and elution with 0.2 M 6-amin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00370a028
更新日期:1986-11-04 00:00:00
abstract::Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00159a024
更新日期:1992-11-10 00:00:00
abstract::Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050866n
更新日期:2005-09-13 00:00:00
abstract::Acid beta-glucosidase is a lysosomal membrane protein that cleaves the O-beta-D-glucosidic linkage of glucosylceramide and aryl-beta-glucosides. Full activity reconstitution of the pure enzyme requires phospholipids and saposin C, an 80 aa activator protein. The deficiency of the enzyme or activator leads to Gaucher d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980785+
更新日期:1998-08-18 00:00:00
abstract::The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-label...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101568j
更新日期:2011-03-29 00:00:00
abstract::Protease inhibition by metastable serine protease inhibitors (serpins) is mediated by one of the largest functional intradomain conformational changes known in biology. In this extensive structural rearrangement, protease-serpin complex formation triggers cleavage of the serpin reactive center loop (RCL), its subseque...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00839
更新日期:2017-01-31 00:00:00
abstract::Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a004
更新日期:1975-07-29 00:00:00
abstract::The properties of Na,K-ATPase phosphoenzymes formed either from ATP in the presence of Mg2+ and Na+ or from Pi in the absence of alkali cations were investigated by biochemical methods and spectrofluorometry employing the styryl dye RH421. We characterized the phosphoenzyme species by their reaction to N-methyl hydrox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980703h
更新日期:1998-09-29 00:00:00
abstract::According to previous X-ray diffraction studies, the D85N mutant of bacteriorhodopsin (bR) with unprotonated Schiff base assumes a protein conformation similar to that in the M photointermediate. We recorded (13)C NMR spectra of [3-(13)C]Ala- and [1-(13)C]Val-labeled D85N and D85N/D96N mutants at ambient temperature t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0015820
更新日期:2000-11-28 00:00:00
abstract::The FTIR spectra for alkyl isocyanides (CNRs) change from a single nu(CN) band centered at approximately 2175 cm(-1) to two peaks at approximately 2075 and approximately 2125 cm(-1) upon binding to sperm whale myoglobin (Mb). The low- and high-frequency peaks have been assigned to in and out conformations, respectivel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100172c
更新日期:2010-06-22 00:00:00
abstract::Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962231h
更新日期:1997-04-15 00:00:00
abstract::The mobility of vesicular stomatitis virus (VSV) G protein on the surface of infected BHK cells was studied by using the technique of fluorescence photobleaching recovery. The fraction of surface G protein that was mobile in that time scale of the measurement (minutes) was at least 75%, a relatively high value among c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00508a047
更新日期:1981-03-03 00:00:00
abstract::Ribonuclease T1 was crystallized in the presence of vanadate(V). The crystal structure was solved by molecular replacement and refined by least-squares methods using stereochemical restraints. The refinement was based on data between 10 and 1.8 A and converged at a crystallographic R factor of 0.137. Except for the su...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00445a014
更新日期:1989-09-19 00:00:00
abstract::Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0105476
更新日期:2001-09-25 00:00:00
abstract::Nitric oxide synthases (NOSs) are proposed to generate NO and citrulline from L-arginine in two steps: initial N-hydroxylation to generate Nomega-hydroxyarginine (NOHA) followed by a three-electron oxidation of the hydroxylated nitrogen to form products. Both steps consume NADPH and may involve heme iron-based activat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971414g
更新日期:1997-09-09 00:00:00
abstract::The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9808971
更新日期:1998-11-03 00:00:00
abstract::ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900760n
更新日期:2009-10-27 00:00:00
abstract::The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00526a028
更新日期:1981-11-10 00:00:00
abstract::Lipoprotein lipase (LPL) is a dimeric enzyme that is responsible for clearing triglyceride-rich lipoproteins from the blood. Although LPL plays a key role in cardiovascular health, an experimentally derived three-dimensional structure has not been determined. Such a structure would aid in understanding mutations in LP...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01009
更新日期:2018-01-16 00:00:00
abstract::Human frataxin has a vital role in the biosynthesis of iron-sulfur (Fe-S) clusters in mitochondria, and its deficiency causes the neurodegenerative disease Friedreich's ataxia. Proposed functions for frataxin in the Fe-S pathway include iron donation to the Fe-S cluster machinery and regulation of cysteine desulfurase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400443n
更新日期:2013-09-03 00:00:00
abstract::The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729211
更新日期:1998-04-07 00:00:00
abstract::Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952521i
更新日期:1996-04-16 00:00:00
abstract::To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00633
更新日期:2016-11-08 00:00:00
abstract::The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00120a020
更新日期:1992-02-11 00:00:00
abstract::According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952518z
更新日期:1996-05-28 00:00:00
abstract::In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01063
更新日期:2017-03-21 00:00:00
abstract::The triplet lifetime and absorption anisotropy decay of eosin-labeled band 3 was measured in resealed erythrocyte ghosts. Membrane potentials were generated by the addition of valinomycin in the presence of a K+ gradient. Neither negative nor positive membrane potentials had any detectable effect on the rotational dif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00134a016
更新日期:1992-05-19 00:00:00
abstract::Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00190a018
更新日期:1994-06-21 00:00:00
abstract::The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00492a022
更新日期:1990-10-09 00:00:00
abstract::Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a044
更新日期:1989-01-10 00:00:00