DNA-binding and oligomerization studies of the manganese(II) metalloregulatory protein MntR from Bacillus subtilis.

Abstract:

:The metalloregulatory protein MntR from Bacillus subtilis acts as a transcriptional regulator of manganese homeostasis. MntR is a member of a subfamily of DtxR-related proteins that perform analogous regulatory functions in a variety of pathogenic organisms. Metal ions activate MntR to bind DNA and repress the transcription of the mntH gene, which encodes for a proton-coupled metal ion transporter. Size-exclusion chromatography and sedimentation equilibrium ultracentrifugation studies show that apo MntR is predominantly a homodimer in solution. Using fluorescence anisotropy measurements, the DNA binding properties of MntR have been examined. In the strict absence of divalent transition metal ions MntR has a low affinity for the mntH control sequence (K(d) > 8.0 microM). However, binding of MntR is stimulated by the presence of Mn(2+) and Cd(2+) to generate high affinity binding with K(d) values of 16.0 and 7.3 nM, respectively. MntR is also shown to bind the mntH control sequence in the presence of other divalent transition metals, including Ni(2+), Cu(2+), and Zn(2+), but with much lower affinity (K(d) approximately 1.3-2.3 microM). The data here demonstrate that differences in metal-activated DNA binding plays a role in the mechanism of manganese(II)-selective transcription factors and that the oligomerization of MntR is metal-independent, which distinguishes this protein from iron(II)-responsive homologues in the DtxR protein family.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Lieser SA,Davis TC,Helmann JD,Cohen SM

doi

10.1021/bi0350248

subject

Has Abstract

pub_date

2003-11-04 00:00:00

pages

12634-42

issue

43

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

杂志文章
  • Characterization of a protein-generated O₂ binding pocket in PqqC, a cofactorless oxidase catalyzing the final step in PQQ production.

    abstract::PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1015474

    authors: RoseFigura JM,Puehringer S,Schwarzenbacher R,Toyama H,Klinman JP

    更新日期:2011-03-08 00:00:00

  • Aggregation studies on fluorescein-coupled cobra venom phospholipase A2.

    abstract::Phospholipase A2 from Naja naja naja venom (Indian cobra) undergoes a concentration-dependent aggregation, and at an assay concentration of 1 microgram mL-1, it exists as a monomer. However, there is some evidence that the enzyme is actually active as a dimer or higher order aggregate. Previous attempts to determine t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00343a018

    authors: Hazlett TL,Dennis EA

    更新日期:1985-10-22 00:00:00

  • Energy landscape of the Michaelis complex of lactate dehydrogenase: relationship to catalytic mechanism.

    abstract::Lactate dehydrogenase (LDH) catalyzes the interconversion between pyruvate and lactate with nicotinamide adenine dinucleotide (NAD) as a cofactor. Using isotope-edited difference Fourier transform infrared spectroscopy on the "live" reaction mixture (LDH·NADH·pyruvate ⇌ LDH·NAD(+)·lactate) for the wild-type protein an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500215a

    authors: Peng HL,Deng H,Dyer RB,Callender R

    更新日期:2014-03-25 00:00:00

  • Electron transfer within nitrogenase: evidence for a deficit-spending mechanism.

    abstract::The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi201003a

    authors: Danyal K,Dean DR,Hoffman BM,Seefeldt LC

    更新日期:2011-11-01 00:00:00

  • Jeltraxin, a frog egg jelly glycoprotein, has calcium-dependent lectin properties and is related to human serum pentraxins CRP and SAP.

    abstract::The egg jelly that encapsulates amphibian eggs is essential for fertilization, but its molecular composition and roles remain largely unknown. We identified a calcium-dependent lectin from the pentraxin superfamily in the egg jelly coat from the South American burrowing frog, Lepidobatrachus laevis. This lectin, jeltr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi035314o

    authors: Peavy TR,Hernandez C,Carroll EJ Jr

    更新日期:2003-11-11 00:00:00

  • Cooperative binding of polyamines induces the Escherichia coli single-strand binding protein-DNA binding mode transitions.

    abstract::The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00141a029

    authors: Wei TF,Bujalowski W,Lohman TM

    更新日期:1992-07-07 00:00:00

  • Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light.

    abstract::Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a mea...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00549a026

    authors: Seawell PC,Simon TJ,Ganesan AK

    更新日期:1980-04-15 00:00:00

  • Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin.

    abstract::The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981699c

    authors: Betlach MC,Kealey JT,Ashley GW,McDaniel R

    更新日期:1998-10-20 00:00:00

  • Variable-temperature spectroelectrochemical study of horseradish peroxidase.

    abstract::The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00009a017

    authors: Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

    更新日期:1995-03-07 00:00:00

  • Electron spin echo envelope modulation study of oxygenated iron-cobalt hybrid hemoglobins reveals molecular features analogous to those of the oxy ferrous protein.

    abstract::Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00020a036

    authors: Lee HC,Peisach J,Tsuneshige A,Yonetani T

    更新日期:1995-05-23 00:00:00

  • Behavior of fragmented calcium (II) adenosine triphosphatase from sarcoplasmic reticulum in detergent solution.

    abstract::The behavior of Ca2+-ATPase from sarcoplasmic reticulum in detergent solution was compared with that of Ca2+-ATPase which had been cleaved in half by limited trypsin digestion. Attempts to dissociate the fragments (I and II) with an excess of detergent micelles demonstrated that fragments I and II are structurally dep...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00612a028

    authors: Rizzolo LJ,Tanford C

    更新日期:1978-09-19 00:00:00

  • Effect of salt and pH on the reductive half-reaction of Mycobacterium tuberculosis FprA with NADPH.

    abstract::Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi702250h

    authors: Pennati A,Zanetti G,Aliverti A,Gadda G

    更新日期:2008-03-18 00:00:00

  • FeNO structure in distal pocket mutants of myoglobin based on resonance Raman spectroscopy.

    abstract::FeNO vibrational frequencies were investigated for a series of myoglobin mutants using isotope-edited resonance Raman spectra of (15/14)NO adducts, which reveal the FeNO and NO stretching modes. The latter give rise to doublet bands, as a result of Fermi resonances with coincident porphyrin vibrations; these doublets ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026395b

    authors: Coyle CM,Vogel KM,Rush TS 3rd,Kozlowski PM,Williams R,Spiro TG,Dou Y,Ikeda-Saito M,Olson JS,Zgierski MZ

    更新日期:2003-05-06 00:00:00

  • Aggregation-induced activation of the epidermal growth factor receptor protein tyrosine kinase.

    abstract::Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00085a004

    authors: Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

    更新日期:1993-08-31 00:00:00

  • Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions.

    abstract::The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026378m

    authors: Sengupta K,Rao BJ

    更新日期:2002-12-24 00:00:00

  • Key residues for the light regulation of the blue light-activated adenylyl cyclase from Beggiatoa sp.

    abstract::Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal transduction mechanisms in biological photoreceptors. Because of its large increase in enzyme activity in the light, the BLUF (blue light sensor using flavin adenine dinucleotide)-activated adenylyl cyclase (bPAC) from Be...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500479v

    authors: Stierl M,Penzkofer A,Kennis JT,Hegemann P,Mathes T

    更新日期:2014-08-12 00:00:00

  • Independent heterologous fibrillation of insulin and its B-chain peptide.

    abstract::Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051658y

    authors: Hong DP,Fink AL

    更新日期:2005-12-20 00:00:00

  • Complex of human apolipoprotein C-1 with phospholipid: thermodynamic or kinetic stability?

    abstract::Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dimyristoyl phosphatidylcholine (DMPC) reveals a novel mechanism of lipoprotein stabilization that is based on kinetics rather than thermodynamics. Far-UV CD melting curves recorded at several heating/cooling rates from 0.047 to 1.34 K/min show ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi025588w

    authors: Gursky O,Ranjana,Gantz DL

    更新日期:2002-06-11 00:00:00

  • Structural and functional properties of human hemoglobins reassembled after synthesis in Escherichia coli.

    abstract::Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981986g

    authors: Hui HL,Kavanaugh JS,Doyle ML,Wierzba A,Rogers PH,Arnone A,Holt JM,Ackers GK,Noble RW

    更新日期:1999-01-19 00:00:00

  • Investigation of the active site cysteine residue of rat liver mitochondrial aldehyde dehydrogenase by site-directed mutagenesis.

    abstract::To determine the active site cysteine residue in aldehyde dehydrogenase, we mutated amino acid residues 49, 162, and 302 of recombinantly expressed rat liver mitochondrial (class 2) aldehyde dehydrogenase. The C49A and C162A mutants were fully active tetrameric enzymes, although the C162A mutant was found to be highly...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00008a025

    authors: Farrés J,Wang TT,Cunningham SJ,Weiner H

    更新日期:1995-02-28 00:00:00

  • Self-association of linker histone H5 and of its globular domain: evidence for specific self-contacts.

    abstract::The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980716v

    authors: Carter GJ,van Holde K

    更新日期:1998-09-08 00:00:00

  • Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: a disulfide mapping study.

    abstract::The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701832b

    authors: Swain KE,Falke JJ

    更新日期:2007-12-04 00:00:00

  • Cysteine biosynthetic enzymes are the pieces of a metabolic energy pump.

    abstract::Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi025953j

    authors: Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

    更新日期:2002-07-02 00:00:00

  • Chromatin condensing functions of the linker histone C-terminal domain are mediated by specific amino acid composition and intrinsic protein disorder.

    abstract::Linker histones bind to the nucleosomes and linker DNA of chromatin fibers, causing changes in linker DNA structure and stabilization of higher order folded and oligomeric chromatin structures. Linker histones affect chromatin structure acting primarily through their approximately 100-residue C-terminal domain (CTD). ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801636y

    authors: Lu X,Hamkalo B,Parseghian MH,Hansen JC

    更新日期:2009-01-13 00:00:00

  • Structure of glucagon-like peptide (7-36) amide in a dodecylphosphocholine micelle as determined by 2D NMR.

    abstract::We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00178a009

    authors: Thornton K,Gorenstein DG

    更新日期:1994-03-29 00:00:00

  • GliP, a multimodular nonribosomal peptide synthetase in Aspergillus fumigatus, makes the diketopiperazine scaffold of gliotoxin.

    abstract::The fungal metabolite gliotoxin has a redox-active disulfide bridge spanning carbons 3 and 6 of a diketopiperazine (DKP) scaffold. The proposed DKP synthetase, GliP, from Aspergillus fumigatus Af293, is a three module (A1-T1-C1-A2-T2-C2-T3) 236 kDa protein that can be overproduced in soluble form in Escherichia coli. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061845b

    authors: Balibar CJ,Walsh CT

    更新日期:2006-12-19 00:00:00

  • Thermal difference circular dichroism of Pf1 filamentous virus and effects of mercury(II), silver(I), and copper(II).

    abstract::The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00410a010

    authors: Casadevall A,Day LA

    更新日期:1988-05-17 00:00:00

  • Structural and functional analysis of NADPH-cytochrome P-450 reductase from human liver: complete sequence of human enzyme and NADPH-binding sites.

    abstract::The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00447a054

    authors: Haniu M,McManus ME,Birkett DJ,Lee TD,Shively JE

    更新日期:1989-10-17 00:00:00

  • Enzymic and physicochemical properties of Streptomyces griseus trypsin.

    abstract::Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00677a010

    authors: Olafson RW,Smillie LB

    更新日期:1975-03-25 00:00:00

  • pH dependence and structural interpretation of the reactions of Coprinus cinereus peroxidase with hydrogen peroxide, ferulic acid, and 2,2'-azinobis.

    abstract::Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970387r

    authors: Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

    更新日期:1997-08-05 00:00:00