Fluorescent oligonucleotides and deoxynucleotide triphosphates: preparation and their interaction with the large (Klenow) fragment of Escherichia coli DNA polymerase I.

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...

journal_title：Biochemistry

authors： Cocco MJ,Kao YH,Phillips AT,Lecomte JT

更新日期：1992-07-21 00:00:00

abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

journal_title：Biochemistry

authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

更新日期：2001-08-21 00:00:00

abstract：:Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides comp...

journal_title：Biochemistry

authors： Broda M,Kierzek E,Gdaniec Z,Kulinski T,Kierzek R

更新日期：2005-08-16 00:00:00

abstract：:The binding of formate ion, a substrate for the peroxidatic reaction of catalase, has been investigated by magnetic resonance techniques. Comparative studies of formate binding to ferric myoglobin have also been performed. The nuclear magnetic relaxation (NMR) rate of formate and water protons is enhanced by the prese...

journal_title：Biochemistry

authors： Hershberg RD,Chance B

更新日期：1975-08-26 00:00:00

abstract：:Neutrophil elastase (NE), a mediator of inflammation, binds with high affinity numerous anionic molecules including suramin, a polysulfated naphthylurea, which inhibits it with a Ki of 0.2 microM and a 4:1 suramin:NE stoichiometry and thus constitutes a potential therapeutic agent. In an attempt to locate the suramin ...

journal_title：Biochemistry

authors： Mély Y,Cadène M,Sylte I,Bieth JG

更新日期：1997-12-16 00:00:00

abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...

journal_title：Biochemistry

authors： Kusumi A,Tsuji A,Murata M,Sako Y,Yoshizawa AC,Kagiwada S,Hayakawa T,Ohnishi S

更新日期：1991-07-02 00:00:00

abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...

journal_title：Biochemistry

authors： Jha S,Snell JM,Sheftic SR,Patil SM,Daniels SB,Kolling FW,Alexandrescu AT

更新日期：2014-01-21 00:00:00

abstract：:The covalent modification of chicken liver fatty acid synthase by 4-(1-pyrenyl)butyryl-CoA (PBA-CoA), a fluorescent analogue of acetyl- and malonyl-CoA, has been studied. The binding isotherms and the kinetics of inactivation suggest 2 mol of PBA-CoA/mol of enzyme is specifically incorporated into the enzyme. Two clas...

journal_title：Biochemistry

authors： Anderson VE,Hammes GG

更新日期：1983-06-07 00:00:00

abstract：:Hypochlorous acid (HOCl) is a powerful oxidant generated from H(2)O(2) and Cl(-) by the heme enzyme myeloperoxidase, which is released from activated leukocytes. HOCl possesses potent antibacterial properties, but excessive production can lead to host tissue damage that is implicated in a wide range of human diseases ...

journal_title：Biochemistry

authors： Pattison DI,Davies MJ

更新日期：2006-07-04 00:00:00

abstract：:Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...

journal_title：Biochemistry

authors： Momb J,Thomas PW,Breece RM,Tierney DL,Fast W

更新日期：2006-11-07 00:00:00

abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

journal_title：Biochemistry

authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

更新日期：2001-01-09 00:00:00

abstract：:The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...

journal_title：Biochemistry

authors： Kleinberg ME,Malech HL,Mital DA,Leto TL

更新日期：1994-03-08 00:00:00

abstract：:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...

journal_title：Biochemistry

authors： Greene FC

更新日期：1975-02-25 00:00:00

abstract：:Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...

journal_title：Biochemistry

authors： Aizawa Y,Sugiura Y,Morii T

更新日期：1999-02-02 00:00:00

abstract：:The 35000-Mr beta-adrenergic receptor mRNA binding protein (beta ARB) is induced by beta-adrenergic agonists and binds to G-protein-linked receptor mRNAs that exhibit agonist-induced destabilization. Recently, we identified a 20-nucleotide, AU-rich region in the 3'-untranslated region of the hamster beta 2-adrenergic ...

journal_title：Biochemistry

authors： Tholanikunnel BG,Raymond JR,Malbon CC

更新日期：1999-11-23 00:00:00

abstract：:The limited proteolysis approach was used to analyze the conformational features of human growth hormone (hGH) under acidic solvent conditions (A-state). Pepsin was used as the proteolytic probe because of its poor substrate specificity and its activity at low pH. Limited proteolysis of hGH in its A-state results in a...

journal_title：Biochemistry

authors： Spolaore B,Polverino de Laureto P,Zambonin M,Fontana A

更新日期：2004-06-01 00:00:00

abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...

journal_title：Biochemistry

authors： Nadal RC,Davies P,Brown DR,Viles JH

更新日期：2009-09-29 00:00:00

abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...

journal_title：Biochemistry

authors： Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice EC

更新日期：1988-07-12 00:00:00

abstract：:Flavo-diiron proteins (FDPs) function as anaerobic nitric oxide scavengers in some microorganisms, catalyzing reduction of nitric to nitrous oxide. The FDP from Thermotoga maritima can be prepared in a deflavinated form with an intact diferric site (deflavo-FDP). Hayashi et al. [(2010) Biochemistry 49, 7040-7049] repo...

journal_title：Biochemistry

authors： Caranto JD,Weitz A,Giri N,Hendrich MP,Kurtz DM Jr

更新日期：2014-09-09 00:00:00

abstract：:Acyl-CoA:cholesterol O-acyltransferase (EC 2.3.1.26) (ACAT) catalyzes the intracellular synthesis of cholesteryl esters from cholesterol and fatty acyl-CoA at neutral pH. Despite the probable pathophysiologic role of ACAT in vascular cholesteryl ester accumulation during atherogenesis, its mechanism of action and its ...

journal_title：Biochemistry

authors： Kinnunen PM,DeMichele A,Lange LG

更新日期：1988-09-20 00:00:00

abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...

journal_title：Biochemistry

authors： Jacobson GR,Takacs BJ,Rosenbusch JP

更新日期：1976-06-01 00:00:00

abstract：:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are...

journal_title：Biochemistry

authors： Linske-O'Connell LI,Sherman F,McLendon G

更新日期：1995-05-30 00:00:00

abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...

journal_title：Biochemistry

authors： Tao T,Lamkin M

更新日期：1981-08-18 00:00:00

abstract：:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to th...

journal_title：Biochemistry

authors： Ikemoto N,Antoniu B,Kang JJ,Mészáros LG,Ronjat M

更新日期：1991-05-28 00:00:00

abstract：:Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...

journal_title：Biochemistry

authors： Mollwitz B,Brunk E,Schmitt S,Pojer F,Bannwarth M,Schiltz M,Rothlisberger U,Johnsson K

更新日期：2012-02-07 00:00:00

abstract：:The hydrogen exchange behavior of the N-terminal helical segment in horse heart cytochrome c was studied in both the reduced and the oxidized forms by use of two-dimensional nuclear magnetic resonance methods. The amide protons of the first six residues are not H bonded and exchange rapidly with solvent protons. The m...

journal_title：Biochemistry

authors： Wand AJ,Roder H,Englander SW

更新日期：1986-03-11 00:00:00

abstract：:We have developed a new genomic sequencing method for detecting, with resolution at the nucleotide level, the interstrand DNA cross-links induced by 4,5',8-trimethylpsoralen along single-copy genes in mammalian cells. The cross-links (diadducts) initially formed are converted into monoadducts by alkali reversal prior ...

journal_title：Biochemistry

authors： Komura J,Ikehata H,Hosoi Y,Riggs AD,Ono T

更新日期：2001-04-03 00:00:00

abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

journal_title：Biochemistry

authors： Miyao-Tokutomi M,Inoue Y

更新日期：1992-01-21 00:00:00

abstract：:Comparative primary structural analyses have begun to elucidate polymorphic residues and segments of the class I antigens of the major histocompatibility complex, at least some of which presumably contribute to determinants important in immune recognition events. HLA-A2 structural variants have been described which ar...

journal_title：Biochemistry

authors： Krangel MS,Taketani S,Biddison WE,Strong DM,Strominger JL

更新日期：1982-11-23 00:00:00