当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Site specific combinations of stabilizing and destabilizing amino acid replacements in yeast cytochrome c: in vivo and in vitro effects.

Site specific combinations of stabilizing and destabilizing amino acid replacements in yeast cytochrome c: in vivo and in vitro effects.

Abstract：

:Oligonucleotide-directed mutagenesis in vivo was used to create destabilizing site specific changes at position 6 in iso-1-cytochrome c in Saccharomyces cerevisiae. These changes were made in combination with the stabilizing N52I substitution. The resulting proteins showed that a variety of forces and interactions are responsible for these destabilizations. Increasing side chain size was the strongest predictor of decreases in cytochrome c levels in vivo. With intermediate size replacements, increasing hydrophobicity correlated with the proteins' thermostability. Some differences in protein levels in vivo could not be explained by side chain size and hydrophobicity alone. Therefore, specific interactions of individual amino acids may also be involved. The N52I-stabilizing mutation tended to increase the protein levels to the same degree relative to the amino acid at position 6. These stabilized cytochromes had an increased specific activity when compared to the series with the original N52. Strains with these altered cytochromes c showed temperature sensitivities for protein levels and function. Thermodynamic measurements in vivo of the WT (C102A), N52I, G6A, G6A N52I, and G6S N52I correlated with the in vivo data. The variant G6A N52I showed additivity (Wells, 1990) of the Cm's and delta delta G's of unfolding for guanidine hydrochloride denaturation.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Linske-O'Connell LI,Sherman F,McLendon G

doi

10.1021/bi00021a023

subject

Has Abstract

pub_date

1995-05-30 00:00:00

pages

7103-12

issue

21

eissn

0006-2960

issn

1520-4995

journal_volume

34

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Rearrangement of domain elements of the Ca-ATPase in cardiac sarcoplasmic reticulum membranes upon phospholamban phosphorylation.

    abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990599j

    authors： Negash S,Huang S,Squier TC

    更新日期：1999-06-22 00:00:00

  • Engineering the zinc binding site of human carbonic anhydrase II: structure of the His-94-->Cys apoenzyme in a new crystalline form.

    abstract：:The structure of the His-94-->Cys variant of human carbonic anhydrase II (CAII) has been determined by X-ray crystallographic methods to a resolution of 2.3 A with a final crystallographic R factor of 0.155. This variant of CAII crystallizes in orthorhombic space group P2(1)2(1)2(1) which is the first example of a new...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00057a015

    authors： Alexander RS,Kiefer LL,Fierke CA,Christianson DW

    更新日期：1993-02-16 00:00:00

  • Cholesterol efflux from macrophage foam cells is enhanced by active phospholipid transfer protein through generation of two types of acceptor particles.

    abstract：:Phospholipid transfer protein (PLTP) is expressed by macrophage-derived foam cells in human atherosclerotic lesions, suggesting a regulatory role for PLTP in cellular cholesterol homeostasis. However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. PLTP is present in plasma as two for...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700833h

    authors： Vikstedt R,Metso J,Hakala J,Olkkonen VM,Ehnholm C,Jauhiainen M

    更新日期：2007-10-23 00:00:00

  • A new omega-conotoxin that targets N-type voltage-sensitive calcium channels with unusual specificity.

    abstract：:A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002871r

    authors： Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

    更新日期：2001-12-04 00:00:00

  • Signal recognition particle (SRP) and SRP receptor: a new paradigm for multistate regulatory GTPases.

    abstract：:The GTP-binding proteins or GTPases comprise a superfamily of proteins that provide molecular switches in numerous cellular processes. The "GTPase switch" paradigm, in which a GTPase acts as a bimodal switch that is turned "on" and "off" by external regulatory factors, has been used to interpret the regulatory mechani...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9006989

    authors： Shan SO,Schmid SL,Zhang X

    更新日期：2009-07-28 00:00:00

  • Characterization of multiple forms of the Ah receptor: recognition of a dioxin-responsive enhancer involves heteromer formation.

    abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00225a026

    authors： Gasiewicz TA,Elferink CJ,Henry EC

    更新日期：1991-03-19 00:00:00

  • Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

    abstract：:Biotin synthase catalyzes the conversion of dethiobiotin (DTB) to biotin through the oxidative addition of sulfur between two saturated carbon atoms, generating a thiophane ring fused to the existing ureido ring. Biotin synthase is a member of the radical SAM superfamily, composed of enzymes that reductively cleave S-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201042r

    authors： Taylor AM,Stoll S,Britt RD,Jarrett JT

    更新日期：2011-09-20 00:00:00

  • A kinetic and allosteric model for the acetylcholine transporter-vesamicol receptor in synaptic vesicles.

    abstract：:The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00140a010

    authors： Bahr BA,Clarkson ED,Rogers GA,Noremberg K,Parsons SM

    更新日期：1992-06-30 00:00:00

  • Guanosine tetraphyosphate and its analogues. Chemical synthesis of guanosine 3',5'-dipyrophosphate, deoxyguanosine 3',5'-dipyrophosphate, guanosine 2',5'-bis(methylenediphosphonate), and guanosine 3',5'-bis(methylenediphosphonate).

    abstract：:A new procedure for the synthesis of the pyrophosphate bond has been employed in the preparation of nucleoside dipyrophosphates from nucleoside 3',5'-diphosphates. The method makes use of a powerful phosphorylating agent generated in a mixture of cyanoethyl phosphate, dicyclohexylcarbodiimide, and mesitylenesulfonyl c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00666a012

    authors： Bennett GN,Gough GR,Gilham PT

    更新日期：1976-10-19 00:00:00

  • 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.

    abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9907835

    authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

    更新日期：1999-10-12 00:00:00

  • Large changes in the CRAC segment of gp41 of HIV do not destroy fusion activity if the segment interacts with cholesterol.

    abstract：:The membrane-proximal external region (MPER) of the gp41 fusion protein of HIV is highly conserved among isolates of this virus and is considered a target for vaccine development. This region also appears to play a role in membrane fusion as well as localization of the virus to cholesterol-rich domains in membranes. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8014828

    authors： Vishwanathan SA,Thomas A,Brasseur R,Epand RF,Hunter E,Epand RM

    更新日期：2008-11-11 00:00:00

  • Calcium-induced structural transition in the regulatory domain of human cardiac troponin C.

    abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971223d

    authors： Spyracopoulos L,Li MX,Sia SK,Gagné SM,Chandra M,Solaro RJ,Sykes BD

    更新日期：1997-10-07 00:00:00

  • Raman spectroscopic study of semisynthetic species of cerebroside sulfate: two types of hydrocarbon chain interdigitation.

    abstract：:Raman spectroscopy was used to study the phase behavior of several semisynthetic species of the acidic glycosphingolipid cerebroside sulfate (CBS) which occur in myelin. The C-H stretching mode region at 2800-3100 cm-1 of C18:0-CBS, C24:0-CBS, and C26:0-CBS, and the alpha-hydroxy fatty acid species C18:0h-CBS, was stu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00121a041

    authors： Stevenson CC,Rich NH,Boggs JM

    更新日期：1992-02-18 00:00:00

  • NMR relaxation of protein and water protons in diamagnetic hemoglobin solutions.

    abstract：:We have measured T1 and T2 of protein and water protons in hemoglobin solutions using broad-line pulse techniques; selective excitation and detection methods enabled the intrinsic protein and water relaxation rates, as well as the spin-transfer rate between them, to be obtained at 5, 10, and 20 MHz. Water and protein ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00335a004

    authors： Eisenstadt M

    更新日期：1985-07-02 00:00:00

  • Mutation induced in vitro on a C-8 guanine aminofluorene containing template by a modified T7 DNA polymerase.

    abstract：:We reacted uracil-containing M13mp2 DNA with N-hydroxy-2-aminofluorene to produce a template with N-(deoxyguanosin-8-yl)-2-aminofluorene adducts. This template was hybridized to a non-uracil-containing linear fragment from which the lac z complementing insert had been removed to produce a gapped substrate. DNA synthes...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00433a014

    authors： Sahm J,Turkington E,LaPointe D,Strauss B

    更新日期：1989-04-04 00:00:00

  • Effect of hydrostatic pressure on lysozyme and chymotrypsinogen detected by fluorescence polarization.

    abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00517a002

    authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

    更新日期：1981-07-07 00:00:00

  • The metastasis-associated extracellular matrix protein osteopontin forms transient structure in ligand interaction sites.

    abstract：:Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200291e

    authors： Platzer G,Schedlbauer A,Chemelli A,Ozdowy P,Coudevylle N,Auer R,Kontaxis G,Hartl M,Miles AJ,Wallace BA,Glatter O,Bister K,Konrat R

    更新日期：2011-07-12 00:00:00

  • Functional interactions of recombinant alpha 2 adrenergic receptor subtypes and G proteins in reconstituted phospholipid vesicles.

    abstract：:The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00227a024

    authors： Kurose H,Regan JW,Caron MG,Lefkowitz RJ

    更新日期：1991-04-02 00:00:00

  • Probing adenine rings and backbone linkages using base specific isotope-edited Raman spectroscopy: application to group II intron ribozyme domain V.

    abstract：:Raman difference spectroscopy is used to probe the properties of a 36-nt RNA molecule, "D5", which lies at the heart of the catalytic apparatus in group II introns. For D5 that has all of its adenine residues labeled with (13)C and (15)N and utilizing Raman difference spectroscopy, we identify the conformationally sen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902117w

    authors： Chen Y,Eldho NV,Dayie TK,Carey PR

    更新日期：2010-04-27 00:00:00

  • Interaction of acute-phase-inducible and liver-enriched nuclear factors with the promoter region of the mouse alpha 1-acid glycoprotein gene-1.

    abstract：:The synthesis and secretion of several acute-phase proteins increases markedly following physiological stress. alpha 1-Acid glycoprotein (AGP), a major acute-phase reactant made by the liver, is strongly induced by inflammatory agents such as lipopolysaccharide (LPS). Nuclear run-on assay showed a 17-fold increase in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00122a005

    authors： Alam T,Papaconstantinou J

    更新日期：1992-02-25 00:00:00

  • Room temperature trapping of rhodopsin photointermediates.

    abstract：:By suspending bovine rhodopsin in trehalose-water glass films, it is possible to trap photostates in the light-activation process. Because of the unusually high vitrification temperature of trehalose-water mixtures, this trapping can be accomplished at room temperature. This allows for a facile investigation of the sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00181a004

    authors： Sikora S,Little AS,Dewey TG

    更新日期：1994-04-19 00:00:00

  • Characterization of prostate-specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I.

    abstract：:The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9626158

    authors： Robert M,Gibbs BF,Jacobson E,Gagnon C

    更新日期：1997-04-01 00:00:00

  • Functional role of the conserved active site proline of triosephosphate isomerase.

    abstract：:The importance of the fully conserved active site proline, Pro168, for the reaction mechanism of triosephosphate isomerase (TIM) has been investigated by studying the enzymatic and crystallographic properties of the P168A variant of trypanosomal TIM. In TIM, Pro168 follows the key catalytic residue Glu167, situated at...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061683j

    authors： Casteleijn MG,Alahuhta M,Groebel K,El-Sayed I,Augustyns K,Lambeir AM,Neubauer P,Wierenga RK

    更新日期：2006-12-26 00:00:00

  • Tyr51: Key Determinant of the Low Thermostability of the Colwellia psychrerythraea Cold-Shock Protein.

    abstract：:Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00144

    authors： Lee Y,Kwak C,Jeong KW,Durai P,Ryu KS,Kim EH,Cheong C,Ahn HC,Kim HJ,Kim Y

    更新日期：2018-07-03 00:00:00

  • Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect.

    abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9813577

    authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

    更新日期：1999-01-05 00:00:00

  • Crystallization and partial characterization of prenyltransferase from avian liver.

    abstract：:Prenyltransferase (EC 2.5.1.1) has been obtained from chicken liver in a stable crystalline form. The enzyme has been shown to be homogeneous by polyacrylamide gel electrophoresis at pH 8.4, and by electrophoresis in sodium dodecyl sulfate containing gels. Electrofocusing of the crystalline enzyme results in a single ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00672a009

    authors： Reed BC,Rilling HC

    更新日期：1975-01-14 00:00:00

  • Cadmium-113 nuclear magnetic resonance studies of proteolytic fragments of calmodulin: assignment of strong and weak cation binding sites.

    abstract：:Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00279a001

    authors： Andersson A,Forsén S,Thulin E,Vogel HJ

    更新日期：1983-05-10 00:00:00

  • Ligand binding to heme proteins: the effect of light on ligand binding in myoglobin.

    abstract：:Extended illumination slows the rebinding of CO to myoglobin after photodissociation at cryogenic temperatures. Two types of models have been put forward to explain the effect: motions of the CO within the heme pocket or conformational transitions of the protein. To resolve this ambiguity, we have studied the effect o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00249a030

    authors： Nienhaus GU,Mourant JR,Chu K,Frauenfelder H

    更新日期：1994-11-15 00:00:00

  • Mechanism of autophosphorylation of mycobacterial PknB explored by molecular dynamics simulations.

    abstract：:Mycobacterial Ser/Thr kinase, PknB, is essential for the growth of the pathogen. Unphosphorylated PknB is catalytically inactive, and its activation requires autophosphorylation of Thr residues on the activation loop. Autophosphorylation can in principle take place via two distinct mechanisms. Intermolecular trans aut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500245v

    authors： Damle NP,Mohanty D

    更新日期：2014-07-22 00:00:00

  • New clevable photoreactive heterobifunctional cross-linking reagents for studying membrane organization.

    abstract：:The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00560a007

    authors： Jaffe CL,Lis H,Sharon N

    更新日期：1980-09-16 00:00:00