New clevable photoreactive heterobifunctional cross-linking reagents for studying membrane organization.

Abstract:

:The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N'-oxysuccinimide ester (3), and N-[4-(p-azidophenylazo)-benzoyl]-11-aminoundecyl-N'-oxysuccinimide ester (4). Two photoaffinity-directed cross-linking agents were prepared by attaching reagents 1 and 2 via their activated ester groups to soybean agglutinin (subunit Mr 30 000). Irradiation of the lectin derivatives resulted in a decrease in their absorption spectra at 360 nm due to photolysis of the bound reagents. Cross-linking of soybean agglutinin subunits following irradiation of the soybean agglutinin derivative to which reagent 2 had been coupled was observed by the appearance of new Coomassie blue staining material (60 000, 90 000, and 120 000 daltons) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. On polyacrylamide gel electrophoresis in the absence of sodium dodecyl sulfate, soybean agglutinin oligomers were observed. Cleavage of the cross-linked soybean agglutinin with 0.1 M sodium dithionite for 25 min at room temperature resulted in the disappearance of the high molecular weight bands and an increase in the amount of uncross-linked material. The use of the photoaffinity-directed agents probing membrane organization is discussed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Jaffe CL,Lis H,Sharon N

doi

10.1021/bi00560a007

subject

Has Abstract

pub_date

1980-09-16 00:00:00

pages

4423-9

issue

19

eissn

0006-2960

issn

1520-4995

journal_volume

19

pub_type

杂志文章
  • Glyoxylate carboligase: a unique thiamin diphosphate-dependent enzyme that can cycle between the 4'-aminopyrimidinium and 1',4'-iminopyrimidine tautomeric forms in the absence of the conserved glutamate.

    abstract::Glyoxylate carboligase (GCL) is a thiamin diphosphate (ThDP)-dependent enzyme, which catalyzes the decarboxylation of glyoxylate and ligation to a second molecule of glyoxylate to form tartronate semialdehyde (TSA). This enzyme is unique among ThDP enzymes in that it lacks a conserved glutamate near the N1' atom of Th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi300893v

    authors: Nemeria N,Binshtein E,Patel H,Balakrishnan A,Vered I,Shaanan B,Barak Z,Chipman D,Jordan F

    更新日期:2012-10-09 00:00:00

  • Coordinating role of His216 in MgATP binding and cleavage in pyruvate carboxylase.

    abstract::His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi4016814

    authors: Adina-Zada A,Jitrapakdee S,Wallace JC,Attwood PV

    更新日期:2014-02-18 00:00:00

  • Bilayer interactions of ether- and ester-linked phospholipids: dihexadecyl- and dipalmitoylphosphatidylcholines.

    abstract::Mixed phospholipid systems of ether-linked 1,2-dihexadecylphosphatidylcholine (DHPC) and ester-linked 1,2-dipalmitoylphosphatidylcholine (DPPC) have been studied by differential scanning calorimetry and X-ray diffraction. At maximum hydration (60 wt % water), DHPC shows three reversible transitions: a main (chain melt...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00395a006

    authors: Kim JT,Mattai J,Shipley GG

    更新日期:1987-10-20 00:00:00

  • G Protein betagamma dimer formation: Gbeta and Ggamma differentially determine efficiency of in vitro dimer formation.

    abstract::The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0504254

    authors: Dingus J,Wells CA,Campbell L,Cleator JH,Robinson K,Hildebrandt JD

    更新日期:2005-09-06 00:00:00

  • The basic helix-loop-helix region of human neurogenin 1 is a monomeric natively unfolded protein which forms a "fuzzy" complex upon DNA binding.

    abstract::Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi901616z

    authors: Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

    更新日期:2010-03-02 00:00:00

  • Mapping the bimolecular interface of the parathyroid hormone (PTH)-PTH1 receptor complex: spatial proximity between Lys(27) (of the hormone principal binding domain) and leu(261) (of the first extracellular loop) of the human PTH1 receptor.

    abstract::In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000195n

    authors: Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

    更新日期:2000-07-18 00:00:00

  • Self-association of linker histone H5 and of its globular domain: evidence for specific self-contacts.

    abstract::The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980716v

    authors: Carter GJ,van Holde K

    更新日期:1998-09-08 00:00:00

  • Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering.

    abstract::Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.8b00719

    authors: Tong X,Li T,Li C,Wang LX

    更新日期:2018-09-04 00:00:00

  • Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase.

    abstract::This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048606d

    authors: Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

    更新日期:2004-11-09 00:00:00

  • C5 cytosine methylation at CpG sites enhances sequence selectivity of mitomycin C-DNA bonding.

    abstract::We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi991307h

    authors: Li VS,Reed M,Zheng Y,Kohn H,Tang M

    更新日期:2000-03-14 00:00:00

  • Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.

    abstract::The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia coli-derived rat intestinal fatty acid-binding protein (I-FABP) have been characterized and compared using amide 15N relaxation and 1H exchange NMR measurements. The amide 1H/15N resonances for apo and holo I-FABP were assigned at 2...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962018l

    authors: Hodsdon ME,Cistola DP

    更新日期:1997-02-25 00:00:00

  • Insight into the catalysis of hydrolysis of four newly synthesized substrates by papain: a proton inventory study.

    abstract::We synthesized the following four new peptide substrates, Suc-Phe-Leu-pNA, Suc-Phe-Leu-NMec, Suc-Phe-Leu-ONPh, and Pht-Phe-Leu-pNA, and we applied the proton inventory method to their hydrolysis by papain. Useful relationships between the rate constants of the catalytic reaction have been established and contributed t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001615b

    authors: Theodorou LG,Lymperopoulos K,Bieth JG,Papamichael EM

    更新日期:2001-04-03 00:00:00

  • Structural characterization and optimization of antibody-selected phage library mimotopes of an antigen associated with autoimmune recurrent thrombosis.

    abstract::The presence of high titers of anti-cardiolipin antibodies (ACA's) of autoimmune origin, which are known to bind to plasma beta2-glycoprotein I (aka apolipoprotein H), correlates clinically with autoimmune recurrent thrombosis. Soluble beta2-glycoprotein I binds to solid-phase ACA (immobilized on a surface plasmon res...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9807207

    authors: Sem DS,Baker BL,Victoria EJ,Jones DS,Marquis D,Yu L,Parks J,Coutts SM

    更新日期:1998-11-17 00:00:00

  • Interactions of myelin basic protein with mixed dodecylphosphocholine/palmitoyllysophosphatidic acid micelles.

    abstract::The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00461a014

    authors: Mendz GL,Brown LR,Martenson RE

    更新日期:1990-03-06 00:00:00

  • Involvement of tryptophan residues at the coenzyme A binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum.

    abstract::Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00417a045

    authors: Shanmugasundaram T,Kumar GK,Wood HG

    更新日期:1988-08-23 00:00:00

  • C-H Bond Cleavage Is Rate-Limiting for Oxidative C-P Bond Cleavage by the Mixed Valence Diiron-Dependent Oxygenase PhnZ.

    abstract::PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00145

    authors: Gama SR,Lo BSY,Séguin J,Pallitsch K,Hammerschmidt F,Zechel DL

    更新日期:2019-12-31 00:00:00

  • Occurrence of 2-5A and RNA degradation in the chick oviduct during rapid estrogen withdrawal.

    abstract::Rapid withdrawal of estrogen from immature chicks, previously stimulated with the hormone, results in the inhibition of transcription of mRNAs of egg white proteins, rapid degradation of existing estrogen-induced mRNAs of egg white proteins, and decline in ribosomes and weight of the oviduct. On rapid withdrawal of es...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00409a019

    authors: Cohrs RJ,Goswami BB,Sharma OK

    更新日期:1988-05-03 00:00:00

  • Spectroscopic and kinetic analysis of a monoclonal IgG cryoglobulin. Effect of mild reduction on cryoprecipitation.

    abstract::The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a n...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00579a027

    authors: Scoville CD,Abraham GN,Turner DH

    更新日期:1979-06-12 00:00:00

  • A fluorescent mutant of the NM domain of the yeast prion Sup35 provides insight into fibril formation and stability.

    abstract::The Sup35 protein of Saccharomyces cerevisiae forms a prion that generates the [PSI(+)] phenotype. Its NM region governs prion status, forming self-seeding amyloid fibers in vivo and in vitro. A tryptophan mutant of Sup35 (NM(F117W)) was used to probe its aggregation. Four indicators of aggregation, Trp 117 maximum em...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9000276

    authors: Palhano FL,Rocha CB,Bernardino A,Weissmuller G,Masuda CA,Montero-Lomelí M,Gomes AM,Chien P,Fernandes PM,Foguel D

    更新日期:2009-07-28 00:00:00

  • Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway.

    abstract::The bifunctional enzyme dehydroquinate dehydratase-shikimate dehydrogenase (DHQ-SDH) catalyzes the dehydration of dehydroquinate to dehydroshikimate and the reduction of dehydroshikimate to shikimate in the shikimate pathway. We report the first crystal structure of Arabidopsis DHQ-SDH with shikimate bound at the SDH ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi060366+

    authors: Singh SA,Christendat D

    更新日期:2006-06-27 00:00:00

  • A conserved threonine within Escherichia coli leucyl-tRNA synthetase prevents hydrolytic editing of leucyl-tRNALeu.

    abstract::Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi002915w

    authors: Mursinna RS,Lincecum TL Jr,Martinis SA

    更新日期:2001-05-08 00:00:00

  • Effect of the inhibitor-resistant M69V substitution on the structures and populations of trans-enamine beta-lactamase intermediates.

    abstract::The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi060990m

    authors: Totir MA,Padayatti PS,Helfand MS,Carey MP,Bonomo RA,Carey PR,van den Akker F

    更新日期:2006-10-03 00:00:00

  • Structure-based design of an intramolecular proton transfer site in murine carbonic anhydrase V.

    abstract::Carbonic anhydrase V (CA V) is a mitochondrial enzyme that catalyzes the hydration of CO2 to produce bicarbonate and a proton. The catalytic properties of wild-type murine CA V suggest the presence of a proton shuttle residue having pKa = 9.2, the role of which is to transfer a proton from zinc-bound water to solution...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9608018

    authors: Heck RW,Boriack-Sjodin PA,Qian M,Tu C,Christianson DW,Laipis PJ,Silverman DN

    更新日期:1996-09-10 00:00:00

  • Carbohydrate chains of human thyrotropin are differentially susceptible to endoglycosidase removal on combined and free polypeptide subunits.

    abstract::The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00392a040

    authors: Ronin C,Papandreou MJ,Canonne C,Weintraub BD

    更新日期:1987-09-08 00:00:00

  • In vitro reconstitution of calf brain microtubules: effects of solution variables.

    abstract::The effects of solution variables on the in vitro reconstitution of calf brain tubulin, purified by the method of Weisenberg et al. (Weisenberg, R. C., Borisy, G. G., and Taylor, E. W. (1968), Biochemistry 7, 4466-4479; Weisenberg, R. C., and Timasheff, S. N. (1970), Biochemistry 9, 4110-4116), as modified by Lee et a...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00627a037

    authors: Lee JC,Timasheff SN

    更新日期:1977-04-19 00:00:00

  • Analyzing the Folding and Binding Steps of an Intrinsically Disordered Protein by Protein Engineering.

    abstract::Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00350

    authors: Bonetti D,Troilo F,Toto A,Brunori M,Longhi S,Gianni S

    更新日期:2017-07-25 00:00:00

  • Functional characterization of the secondary actin binding site of myosin II.

    abstract::The role of the interaction between actin and the secondary actin binding site of myosin (segment 565-579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been studied with proteolytically generated smooth and skeletal muscle myosin subfragment 1 and recombinant Dictyostelium discoideum myosin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi991595h

    authors: Van Dijk J,Furch M,Lafont C,Manstein DJ,Chaussepied P

    更新日期:1999-11-16 00:00:00

  • Synthesis and study of phosphoenolthiopyruvate.

    abstract::Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00404a038

    authors: Sikkema KD,O'Leary MH

    更新日期:1988-02-23 00:00:00

  • Inhibitor and NAD+ binding to poly(ADP-ribose) polymerase as derived from crystal structures and homology modeling.

    abstract::Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972383s

    authors: Ruf A,de Murcia G,Schulz GE

    更新日期:1998-03-17 00:00:00

  • Interaction of fusogenic synthetic peptide with phospholipid bilayers: orientation of the peptide alpha-helix and binding isotherm.

    abstract::We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952547+

    authors: Ishiguro R,Matsumoto M,Takahashi S

    更新日期:1996-04-16 00:00:00