Abstract:
:Neutral oligosaccharides, which accounted for 74% of the total N-linked sugar chains released by hydrazinolysis of rat small intestinal aminopeptidase N, were investigated on a structural basis. They are mainly composed of complex-type sugar chains with tri- and tetraantennary structures, and small amounts of high mannose type sugar chains (7% of the total neutral sugar chains) are also included. The unique feature of the complex-type sugar chains is the most of them contain terminal N-acetylglucosamine residues and blood group H antigenic determinants in their outer chain moieties, and bisecting N-acetylglucosamine residues in their trimannosyl cores. Both the type 1H and type 2H determinants are found, but the former is mainly expressed at the distal portions of the outer chain moieties of the oligosaccharides.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Takasaki S,Erickson RH,Kim YS,Kochibe N,Kobata Adoi
10.1021/bi00101a028subject
Has Abstractpub_date
1991-09-17 00:00:00pages
9102-10issue
37eissn
0006-2960issn
1520-4995journal_volume
30pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The transporter associated with antigen processing (TAP) is a prototype of an asymmetric ATP-binding cassette (ABC) transporter, which uses ATP binding and hydrolysis to translocate peptides from the cytosol to the lumen of the endoplasmic reticulum (ER). Here, we review molecular details of peptide binding and ATP bi...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi300128f
更新日期:2012-06-26 00:00:00
abstract::Atomic resolution ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi0517133 更新日期:2006-02-21 00:00:00
abstract::A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00376a019
更新日期:1987-01-27 00:00:00
abstract::We have studied the binding of hemoglobin to the red cell membrane by centrifugation and fluorescence methods. The intact red cell was labeled with eosin-5-maleimide (EM), which specifically reacts with lysine 430 of band 3. Even though this residue is not part of the cytoplasmic domain of band 3 (cdb3) associated wit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012007e
更新日期:2002-07-09 00:00:00
abstract::Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00844
更新日期:2019-11-19 00:00:00
abstract::We describe a method for isolating milligram quantities of assembly-competent tubulin from the budding yeast Saccharomyces cerevisiae. The tubulin is > 95% purified and free of contaminating enzyme activities. As a result, it has been possible to determine the yeast tubulin equilibrium-binding constant for Mg-GTP and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00085a013
更新日期:1993-08-31 00:00:00
abstract::Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a015
更新日期:1990-03-27 00:00:00
abstract::Taq and T7 DNA polymerases have become basic molecular biology "tools" for DNA sequence analysis. However, Taq, unlike T7 DNA polymerase, is strongly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very different substrate selectivities. Equilibrium binding and rate consta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951682j
更新日期:1996-02-20 00:00:00
abstract::We have used nucleobase substitution and kinetic analysis to test the hypothesis that hammerhead catalysis occurs by a general acid-base mechanism, in which nucleobases are directly involved in deprotonation of the attacking 2'-hydroxyl group and protonation of the 5'-oxygen that serves as the leaving group in the cle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047941z
更新日期:2005-05-31 00:00:00
abstract::Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.8b00944
更新日期:2019-01-29 00:00:00
abstract::SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0490744
更新日期:2004-09-28 00:00:00
abstract::The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049797n
更新日期:2004-06-08 00:00:00
abstract::The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00406a017
更新日期:1988-03-22 00:00:00
abstract::Helicases are enzymes that use energy from nucleoside triphosphate hydrolysis to unwind double-stranded (ds) DNA, a process vital to virtually every phase of DNA metabolism. Helicases have been classified as either 5'-to-3' or 3'-to-5' on the basis of their ability to unwind duplex DNA adjacent to either a 5' or 3' si...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012058b
更新日期:2002-02-19 00:00:00
abstract::When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a025
更新日期:1982-12-07 00:00:00
abstract::Three distinct fibrinolytic enzymes have been purified from the venom of Crotalus basiliscus basiliscus (the Mexican west coast rattlesnake). The high-performance liquid chromatography-based purification comprised the following steps: (a) hydrophobic interaction chromatography; (b) hydroxylapatite chromatography; (c) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00134a003
更新日期:1992-05-19 00:00:00
abstract::The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel. These are associated to homotrimers which are unusually stable, due mostly to hydrophobic interactions between the beta-barrels. In addition, a loop, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981215c
更新日期:1998-11-10 00:00:00
abstract::Native EcoRI DNA methyltransferase (Mtase, Mr 38,050) is proteolyzed by trypsin to generate an intermediate 36-kDa fragment (p36) followed by the formation of two polypeptides of Mr 23,000 and 13,000 (p23 and p13, respectively). Protein sequence analysis of the tryptic fragments indicates that p36 results from removal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00225a030
更新日期:1991-03-19 00:00:00
abstract::The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971022+
更新日期:1997-09-30 00:00:00
abstract::Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0508845
更新日期:2005-08-23 00:00:00
abstract::Proton nuclear magnetic resonance spectra of human hemoglobins in water reveal several exchangeable protons which are indicators of the quaternary structures of both the liganded and unliganded molecules. A comparison of the spectra of normal human adult hemoglobin with those of mutant hemoglobins Chesapeake (FG4alpha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00682a036
更新日期:1975-06-03 00:00:00
abstract::A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00646a028
更新日期:1976-01-13 00:00:00
abstract::The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00344a013
更新日期:1985-11-05 00:00:00
abstract::Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00425a013
更新日期:1988-12-13 00:00:00
abstract::The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00079a029
更新日期:1993-07-20 00:00:00
abstract::Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent stimulators of cell and growth processes. They display high sequence similarity to both the A and B chains of insulin but contain an additional connecting C-domain, which reflects their secretion without specific packaging or precursor conversion....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020084j
更新日期:2002-07-30 00:00:00
abstract::The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00227a024
更新日期:1991-04-02 00:00:00
abstract::The inducible nitric oxide synthase core oxygen domain (iNOS(COD)) is a homodimeric protein complex of ca. 100 kDa. In this work, the subunit disassembly and unfolding of the protein following a pH jump from 7.5 to 2.8 were monitored by on-line rapid mixing in conjunction with electrospray (ESI) time-of-flight mass sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047684y
更新日期:2005-02-22 00:00:00
abstract::UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00230a004
更新日期:1991-04-23 00:00:00
abstract::Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052387u
更新日期:2006-04-11 00:00:00