Abstract：
:BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular mechanism remains poorly understood. Crystal structures of BamA and complementary molecular dynamics simulations have shown that its β-barrel seam (the interface between the first and last barrel strands) is destabilized. This has led to mechanistic models in which the BamA barrel seam functions as a lateral gate that opens and successively accepts β-hairpins from a nascent OMP such that a nascent barrel can bud from BamA. Consistent with this model, disulfide locking of the BamA barrel seam is lethal in Escherichia coli. Here we show that disulfide locking of the BamA barrel has no effect on its ability to catalyze folding of a model OMP into liposomes. However, disulfide trapping experiments indicate that the BamA barrel is highly dynamic in the liposome membranes, with the β-strands at the barrel seam undergoing "register sliding" by more than 14 Å both up and down the membrane. Remarkably, these extreme dynamics were also observed in the BamA barrel in the context of the native E. coli outer membrane. These results are consistent with a model in which the BamA barrel dynamics induce defects in the outer membrane that facilitate insertion of nascent OMPs.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Doerner PA,Sousa MCdoi
10.1021/acs.biochem.7b00281subject
Has Abstractpub_date
2017-06-20 00:00:00pages
3142-3149issue
24eissn
0006-2960issn
1520-4995journal_volume
56pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200518d
更新日期：2011-07-26 00:00:00
abstract：:The precipitation mechanism of alpha-globulin in the presence of myo-inositol hexaphosphate (sodium phytate) was studied in detail. The maximum interaction was found at pH 2.3 where the protein was in a dissociated state having an 8.3S aggregate and a 1.5S monomer. This interaction was predominantly dependent upon the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a035
更新日期：1993-04-06 00:00:00
abstract：:The ras oncogene products (H-Ras, K-Ras, and N-Ras) have been regarded as some of the most promising targets for anticancer drug discovery because their activating mutations are frequently found in human cancers. Nonetheless, molecular targeted therapy for them is currently unavailable. Here, we report the discovery o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00680
更新日期：2018-09-11 00:00:00
abstract：:Many proteins that are destined to reside within the lumen of the peroxisome contain the peroxisomal targeting signal-1 (PTS1), a C-terminal tripeptide approximating the consensus sequence -Ser-Lys-Leu-COO(-). The PTS1 is recognized by the tetratricopeptide repeat (TPR) domains of PEX5, a cytosolic receptor that cycle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027034z
更新日期：2003-02-18 00:00:00
abstract：:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neith...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00327a002
更新日期：1985-03-12 00:00:00
abstract：:Histone H10 differs substantially from H1 in a 37-residue region of the primary structure that is conserved between the usual H1 histones of fish and mammals. Instead, H10 is homologous to H5 in this region. Histones H10 and H5 might be classified together in a histone category distinct from the usual H1. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00511a035
更新日期：1981-04-14 00:00:00
abstract：:As part of a search for organic compounds that selectively target RNA, we found that specific diphenylfuran derivatives, which are related to compounds that bind to the DNA minor groove, bind very strongly to RNA in a manner very sensitive to the structure of the compounds. In extended development of the diphenylfuran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002338b
更新日期：2001-02-06 00:00:00
abstract：:The fetal Alz-50 reactive clone 1 (FAC1) protein exhibits altered expression and subcellular localization during neuronal development and neurodegenerative diseases such as Alzheimer's disease. Using the yeast two-hybrid screen, the human orthologue of Keap1 (hKeap1) was identified as a FAC1 interacting protein. Keap1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0494166
更新日期：2004-09-28 00:00:00
abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00004
更新日期：2018-03-20 00:00:00
abstract：:MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960956f
更新日期：1996-10-08 00:00:00
abstract：:The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991303c
更新日期：1999-09-28 00:00:00
abstract：:The beta-glucosidase from Agrobacterium sp. catalyzes the hydrolysis of beta-glucosides via a covalent alpha-D-glucopyranosyl-enzyme intermediate involving Glu358. Hydrolysis of 2,4-dinitrophenyl beta-D-glucopyranoside by the low activity Glu358Asp mutant of Agrobacterium beta-glucosidase is accompanied by time-depend...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00044a033
更新日期：1995-11-07 00:00:00
abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00656a008
更新日期：1976-06-01 00:00:00
abstract：:hTom20 is an outer mitochondrial membrane receptor involved in protein translocation. The cytosolic domain (aa30-145) and selected truncated versions of this domain were overexpressed and purified to study the structure-function relationship of this protein. Our studies reveal that the secondary structure of the cytos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980746y
更新日期：1998-09-22 00:00:00
abstract：:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00618a017
更新日期：1978-12-12 00:00:00
abstract：:The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RN...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00119a002
更新日期：1992-02-04 00:00:00
abstract：:The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00117a033
更新日期：1992-01-21 00:00:00
abstract：:The structure and backbone dynamics of an extended second transmembrane segment (TM2e) of the human neuronal glycine receptor alpha(1) subunit in sodium dodecyl sulfate micelles were studied by (1)H and (15)N solution-state NMR. The 28-amino acid segment contained the consensus TM2 domain plus part of the linker betwe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026767g
更新日期：2003-04-08 00:00:00
abstract：:Two highly conserved amino acid residues, an arginine and a glutamine, located near the C-terminal end of the gamma subunit, form a "catch" by hydrogen bonding with residues in an anionic loop on one of the three catalytic beta subunits of the bovine mitochondrial F1-ATPase [Abrahams, J. P., Leslie, A. G., Lutter, R.,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701581y
更新日期：2008-01-15 00:00:00
abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00208a015
更新日期：1994-10-25 00:00:00
abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049953q
更新日期：2004-06-29 00:00:00
abstract：:The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00122a019
更新日期：1992-02-25 00:00:00
abstract：:The binding of various forms of vanadate to myosin and myosin subfragment 1 (S-1) was studied by 51V NMR at increasing vanadate concentrations between 0.06 and 1.0 mM. The distribution of the various forms of vanadate in the solution depended on the total concentration of vanadate. At low concentrations, the predomina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00490a029
更新日期：1990-09-25 00:00:00
abstract：:We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00435a011
更新日期：1989-05-02 00:00:00
abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00286
更新日期：2019-06-04 00:00:00
abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962391t
更新日期：1997-03-04 00:00:00
abstract：:Human platelet glycoproteins Ib alpha, Ib beta, V, and IX comprise an interrelated set of molecules (the Ib-V-IX system) that together form a surface adhesion receptor for the ligand, von Willebrand factor. To complete the primary structural characterization of the genes involved in this system, we have analyzed cosmi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00049a028
更新日期：1995-12-12 00:00:00
abstract：:Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00667a007
更新日期：1976-11-02 00:00:00
abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00156a015
更新日期：1992-10-20 00:00:00
abstract：:Purple membranes of Halobacterium halobium were studied by differential scanning calorimetry. No transition was detected at temperatures below 70 degrees C. A small endothermic transition was seen at about 80 degrees C and a larger one at 100 degrees C. The larger transition is the irreversible denaturation of bacteri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00598a026
更新日期：1978-03-07 00:00:00
