Substrate-induced inactivation of a crippled beta-glucosidase mutant: identification of the labeled amino acid and mutagenic analysis of its role.

abstract：:Endogenous lectins induce effects on cell growth by binding to antennae of natural glycoconjugates. These complex carbohydrates often present more than one potential lectin-binding site in a single chain. Using the growth-regulatory interaction of the pentasaccharide of ganglioside GM(1) with homodimeric galectin-1 on...

journal_title：Biochemistry

authors： Siebert HC,André S,Lu SY,Frank M,Kaltner H,van Kuik JA,Korchagina EY,Bovin N,Tajkhorshid E,Kaptein R,Vliegenthart JF,von der Lieth CW,Jiménez-Barbero J,Kopitz J,Gabius HJ

更新日期：2003-12-23 00:00:00

abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

journal_title：Biochemistry

authors： McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

更新日期：2011-09-06 00:00:00

abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...

journal_title：Biochemistry

authors： Petersheim M,Turner DH

更新日期：1983-01-18 00:00:00

abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...

journal_title：Biochemistry

authors： Halling SM,Sanchez-Anzaldo FJ,Fukuda R,Doi RH,Meares CF

更新日期：1977-06-28 00:00:00

abstract：:Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer seg...

journal_title：Biochemistry

authors： Goldberg AF,Moritz OL,Molday RS

更新日期：1995-10-31 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...

journal_title：Biochemistry

authors： Lerch M,Kamimori H,Folkers G,Aguilar MI,Beck-Sickinger AG,Zerbe O

更新日期：2005-06-28 00:00:00

abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

journal_title：Biochemistry

authors： Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

更新日期：1993-08-31 00:00:00

abstract：:P16(INK4A) (also known as P16 and MTS1), a protein consisting exclusively of four ankyrin repeats, is recognized as a tumor suppressor mainly because of the prevalence of genetic inactivation of the p16(INK4A) (or CDKN2A) gene in virtually all types of human cancers. However, it has also been shown that an elevated le...

journal_title：Biochemistry

authors： Li J,Poi MJ,Tsai MD

更新日期：2011-06-28 00:00:00

abstract：:The insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/MPR) is a type I glycoprotein that mediates both the intracellular sorting of lysosomal enzymes bearing mannose 6-phosphate (Man-6-P) residues to the lysosome and the bioavailability of IGF-II. The extracytoplasmic region of the IGF-II/MPR contains...

journal_title：Biochemistry

authors： Marron-Terada PG,Hancock MK,Haskins DJ,Dahms NM

更新日期：2000-03-07 00:00:00

abstract：:PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...

journal_title：Biochemistry

authors： van den Berk LC,Landi E,Walma T,Vuister GW,Dente L,Hendriks WJ

更新日期：2007-11-27 00:00:00

abstract：:Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...

journal_title：Biochemistry

authors： Fu I,Cai Y,Geacintov NE,Zhang Y,Broyde S

更新日期：2017-04-11 00:00:00

abstract：:It is known that aconitase from mammalian mitochondria is only partially active as isolated but may be activated by incubation with iron, ascorbate, and a thiol, or with dithionite. It has been suggested that the added Fe in the activation mixture is essential for activation and that it is incorporated in the enzyme [...

journal_title：Biochemistry

authors： Ramsay RR,Dreyer JL,Schloss JV,Jackson RH,Coles CJ,Beinert H,Cleland WW,Singer TP

更新日期：1981-12-22 00:00:00

abstract：:Proton NMR studies were conducted on the complementary 15-mer duplex d(5'-TACTCTTCTTGACCT).(5'-AGGTCAAGAAGAGTA) (designated as unmodified 15-mer duplex) spanning a portion of the mouse c-Ha-ras protooncogene centered around codon 61. Identical studies were carried out on the same sequence, after specific modification ...

journal_title：Biochemistry

authors： Cho BP,Beland FA,Marques MM

更新日期：1992-10-13 00:00:00

abstract：:A new bifunctional spin-label (BSL) has been synthesized that can be immobilized on the surface of proteins, allowing measurement of rotational motion of proteins by saturation-transfer electron paramagnetic resonance (STEPR). The spin-label contains a photoactivatable azido moiety, a cleavable disulfide, and a nitrox...

journal_title：Biochemistry

authors： Wilcox MD,Parce JW,Thomas MJ,Lyles DS

更新日期：1990-06-19 00:00:00

abstract：:Hypochlorous acid (HOCl) is a powerful oxidant generated from H(2)O(2) and Cl(-) by the heme enzyme myeloperoxidase, which is released from activated leukocytes. HOCl possesses potent antibacterial properties, but excessive production can lead to host tissue damage that is implicated in a wide range of human diseases ...

journal_title：Biochemistry

authors： Pattison DI,Davies MJ

更新日期：2006-07-04 00:00:00

abstract：:Iron-sulfur clusters are ubiquitous protein cofactors with critical cellular functions. The mitochondrial Fe-S assembly complex, which consists of the cysteine desulfurase NFS1 and its accessory protein (ISD11), the Fe-S assembly protein (ISCU2), and frataxin (FXN), converts substrates l-cysteine, ferrous iron, and el...

journal_title：Biochemistry

authors： Bridwell-Rabb J,Fox NG,Tsai CL,Winn AM,Barondeau DP

更新日期：2014-08-05 00:00:00

abstract：:In mammals, citric acid cycle intermediates play a key role in regulating various metabolic processes, such as fatty acid synthesis and glycolysis. Members of the sodium-dependent SLC13 transporter family mediate the transport of di- and tricarboxylates into cells. SLC13 family members have been implicated in lifespan...

journal_title：Biochemistry

authors： Colas C,Pajor AM,Schlessinger A

更新日期：2015-08-11 00:00:00

abstract：:A striking feature of the CLIC (chloride intracellular channel) protein family is the ability of its members to convert between a soluble state and an integral membrane channel form. Direct evidence of the structural transition required for the CLIC protein to autonomously insert into the membrane is lacking, largely ...

journal_title：Biochemistry

authors： Goodchild SC,Howell MW,Littler DR,Mandyam RA,Sale KL,Mazzanti M,Breit SN,Curmi PM,Brown LJ

更新日期：2010-06-29 00:00:00

abstract：:T4 polynucleotide kinase is widely used for 5'-phosphorylation of DNA and RNA oligonucleotide termini, but no natural protein enzyme is capable of 3'-phosphorylation. Here, we report the in vitro selection of deoxyribozymes (DNA enzymes) capable of DNA oligonucleotide 3'-phosphorylation, using a 5'-triphosphorylated R...

journal_title：Biochemistry

authors： Camden AJ,Walsh SM,Suk SH,Silverman SK

更新日期：2016-05-10 00:00:00

abstract：:The complete primary structure of the major component myoglobin from the Arctic minke whale, Balaenoptera acutorostrata, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three ...

journal_title：Biochemistry

authors： Lehman LD,Dwulet FE,Bogardt RA Jr,Jones BN,Gurd FR

更新日期：1977-02-22 00:00:00

abstract：:Liver alcohol dehydrogenase (LADH) carboxymethylated at Cys-46 (CMLADH) forms two different ternary complexes with 4-trans-(N,N-dimethylamino)cinnamaldehyde (DACA). The complex with reduced nicotinamide adenine dinucleotide (NADH) is characterized by a 38-nm red shift of the long-wavelength pi, pi* transition to 436 n...

journal_title：Biochemistry

authors： Dahl KH,Dunn MF

更新日期：1984-12-18 00:00:00

abstract：:Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whos...

journal_title：Biochemistry

authors： Johansson K,Ramaswamy S,Saarinen M,Lemaire-Chamley M,Issakidis-Bourguet E,Miginiac-Maslow M,Eklund H

更新日期：1999-04-06 00:00:00

abstract：:A 16-mer deoxyribonucleotide was labeled at the 5'-end with x-rhodamine and at the 3'-end with fluorescein. Förster resonance energy transfer was used to determine the distribution, P(R), of donor-acceptor distances for the oligomer in three duplex structures (hybridized to complementary strands having 10, 16, and 24 ...

journal_title：Biochemistry

authors： Parkhurst KM,Parkhurst LJ

更新日期：1995-01-10 00:00:00

abstract：:The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...

journal_title：Biochemistry

authors： Lushnikov AY,Potaman VN,Oussatcheva EA,Sinden RR,Lyubchenko YL

更新日期：2006-01-10 00:00:00

abstract：:We systematically and quantitatively investigated the structure and thermodynamics of G-quadruplexes of RNAs and corresponding DNAs of the same sequences under molecular crowding conditions that mimic the high osmotic stress induced by the numerous molecules inside of living cells. Structural analyses demonstrated tha...

journal_title：Biochemistry

authors： Zhang DH,Fujimoto T,Saxena S,Yu HQ,Miyoshi D,Sugimoto N

更新日期：2010-06-01 00:00:00

abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

journal_title：Biochemistry

authors： Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

更新日期：1989-12-12 00:00:00

abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...

journal_title：Biochemistry

authors： Laco GS,Schalk-Hihi C,Lubkowski J,Morris G,Zdanov A,Olson A,Elder JH,Wlodawer A,Gustchina A

更新日期：1997-09-02 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:Intramolecular excimerization of 1,3-di-1-pyrenylpropane [Py(3)Py] was used to assess the fluidity of sarcoplasmic reticulum membranes (SR); on the basis of the spectral data, the probe incorporates completely inside the membrane probably somewhere close to the polar head groups of phospholipid molecules, however not ...

journal_title：Biochemistry

authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

更新日期：1984-09-25 00:00:00