Abstract:
:Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whose structure has been determined and refined at 2.4 A resolution. In addition to the normal structural components of MDHs, the enzyme exhibits extensions at both the N- and C-termini, each of which contains a regulatory disulfide bridge which must be reduced for activation. The N-terminal disulfide motif is inserted in a cleft between the two subunits of the dimer, thereby locking the domains in each subunit. The C-terminal disulfide keeps the C-terminal residues tight to the enzyme surface and blocks access to the active site. Reduction of the N-terminal disulfide would release the stopper between the domains and give the enzyme the necessary flexibility. Simultaneous reduction of the C-terminal disulfide would free the C-terminal residues from binding to the enzyme and make the active site accessible.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Johansson K,Ramaswamy S,Saarinen M,Lemaire-Chamley M,Issakidis-Bourguet E,Miginiac-Maslow M,Eklund Hdoi
10.1021/bi982876csubject
Has Abstractpub_date
1999-04-06 00:00:00pages
4319-26issue
14eissn
0006-2960issn
1520-4995pii
bi982876cjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Streptomyces avermitilis, an industrial organism responsible for the production of the anthelminthic avermectins, harbors a 13.4 kb gene cluster containing 13 unidirectionally transcribed open reading frames corresponding to the apparent biosynthetic operon for the sesquiterpene antibiotic pentalenolactone. The advanc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060419n
更新日期:2006-05-16 00:00:00
abstract::To establish the state of protonation of quinonoid species formed nonenzymically from pyridoxal phosphate (PLP) and diethyl aminomalonate, we have studied absorption spectra of the rapidly established steady-state mixture of species. We have evaluated the formation constant and the spectrum of the mixture of Schiff ba...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a050
更新日期:1988-06-28 00:00:00
abstract::Tyrosinase is a copper monooxygenase containing a coupled dinuclear copper active site (type-3 copper), which catalyzes oxygenation of phenols (phenolase activity) as well as dehydrogenation of catechols (catecholase activity) using O(2) as the oxidant. In this study, catalase activity (conversion of H(2)O(2) to (1/2)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048908f
更新日期:2004-09-14 00:00:00
abstract::Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi5007892
更新日期:2014-08-12 00:00:00
abstract::Mass spectrometry was used to identify the sites of covalent attachment of [(14)C]-17alpha-bromoacetamidopropylestradiol ([(14)C]17BAPE(2), an estradiol agonist) to the ligand-binding domain (LBD) of mouse estrogen receptor alpha (ERalpha). A glutathione S-transferase (GST)-LBD chimera protein was overexpressed in Esc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0205092
更新日期:2002-12-31 00:00:00
abstract::The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a045
更新日期:1995-04-11 00:00:00
abstract::The crystal structure of the catalytic domain of rat DNA polymerase beta revealed that Asp256 is located in proximity to the previously identified active site residues Asp190 and Asp192. We have prepared and kinetically characterized the nucleotidyl transfer activity of wild type and several mutant forms of human and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00049a008
更新日期:1995-12-12 00:00:00
abstract::Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300171s
更新日期:2012-05-01 00:00:00
abstract::The reduction kinetics of cytochrome P-450 are known to be biphasic, with a rapid initial phase and a slower subsequent phase, both of which appear linear in semilogarithmic plots. The present report demonstrates that these biphasic reduction kinetics can be described in terms of a preequilibrium between high- and low...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00535a034
更新日期:1982-03-16 00:00:00
abstract::Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00507a006
更新日期:1981-02-17 00:00:00
abstract::Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00058a013
更新日期:1993-02-23 00:00:00
abstract::The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700384t
更新日期:2007-07-03 00:00:00
abstract::The temporal evolution of the near-infrared stimulated emission band of the special pair excited state (P*) in the reaction center of Rhodobacter sphaeroides has been studied in intracytoplasmic membranes of the antenna-deficient RCO1 mutant at 10 K with a resolution of 30 fs. On the 100-fs time scale the emission ban...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00188a002
更新日期:1994-06-07 00:00:00
abstract::Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.6b00703
更新日期:2016-09-27 00:00:00
abstract::During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700416h
更新日期:2007-07-10 00:00:00
abstract::Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a008
更新日期:1992-05-26 00:00:00
abstract::We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a025
更新日期:1986-11-18 00:00:00
abstract::Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051658y
更新日期:2005-12-20 00:00:00
abstract::The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970122c
更新日期:1997-07-01 00:00:00
abstract::The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00177a004
更新日期:1994-03-22 00:00:00
abstract::The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01199
更新日期:2017-01-17 00:00:00
abstract::The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01084
更新日期:2020-04-07 00:00:00
abstract::The membrane-bound quinoprotein glucose dehydrogenase (mGDH) in Escherichia coli contains pyrroloquinoline quinone (PQQ) and participates in the direct oxidation of D-glucose to D-gluconate by transferring electrons to ubiquinone (UQ). To elucidate the mechanism of ubiquinone reduction by mGDH, we applied a pulse radi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051347n
更新日期:2005-10-18 00:00:00
abstract::Transient kinetic data for D-xylose reduction with NADPH and NADPD and for xylitol oxidation with NADP+ catalyzed by recombinant C298A mutant human aldose reductase at pH 8 have been used to obtain estimates for each of the rate constants in the complete reaction mechanism as outlined for the wild-type enzyme in the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a013
更新日期:1995-11-07 00:00:00
abstract::The peptide alpha 1(III)-CB9 was prepared and purified from human liver, and its amino acid sequence was determined. Automated Edman degradation of the intact peptide and peptides derived from selective cleavage with hydroxylamine and digestions with trypsin, thermolysin, and Staph V8 protease enabled determination of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00512a040
更新日期:1981-04-28 00:00:00
abstract::Clustering of anionic lipids away from zwitterionic ones by cationic antimicrobial agents has recently been established as a mechanism of action of natural small, flexible peptides as well as non-natural synthetic peptide mimics. One of the largest classes of antimicrobial peptides consists of peptides that form catio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100378m
更新日期:2010-05-18 00:00:00
abstract::The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3000462
更新日期:2012-06-26 00:00:00
abstract::The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00695a018
更新日期:1975-12-02 00:00:00
abstract::The interaction of Ca2+ and vanadate with fluorescein isothiocyanate (FITC) labeled sarcoplasmic reticulum (SR) Ca2+-ATPase has been studied by following the kinetics of changes in the reporter group fluorescence and equilibrium fluorescence levels. The vanadate species bound to the enzyme is clearly monomeric orthova...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00428a057
更新日期:1989-01-24 00:00:00
abstract::We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a039
更新日期:1989-06-13 00:00:00