Abstract:
:Human platelet glycoproteins Ib alpha, Ib beta, V, and IX comprise an interrelated set of molecules (the Ib-V-IX system) that together form a surface adhesion receptor for the ligand, von Willebrand factor. To complete the primary structural characterization of the genes involved in this system, we have analyzed cosmid clones for both the glycoprotein V and IX genes and used these clones to localize the two genes by fluorescence in situ hybridization. Both genes were found on the long arm of chromosome 3, but at distinct sites, the GPV gene on 3 band q29 and the GP IX gene on 3 band q21. The transcriptional start site of the GPV gene was defined by "anchored" PCR and primer extension. The GPV gene contains two exons, the first consisting of approximately 37 bases and the second of approximately 3500 bases, interrupted by a single 958 base intron. The GPV transcript has multiple start sites spread over a twenty base region. The 5' flanking region of the GPV gene has a series of potential consensus regulatory elements including GATA, ets, and Sp-1 sites, similar to those found in other described megakaryocyte/platelet genes, including those of the Ib-V-IX system. In assessing the four Ib-V-IX genes as a group, all four have a simple, "intron-depleted" structure with the entire open reading frame of the mature polypeptide located within a single exon.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yagi M,Edelhoff S,Disteche CM,Roth GJdoi
10.1021/bi00049a028subject
Has Abstractpub_date
1995-12-12 00:00:00pages
16132-7issue
49eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Nitrosylhemoglobin (HbFe(II)NO) has been shown to be generated in vivo from the reaction of deoxyHb with NO(*) as well as with nitrite. Despite the physiological importance attributed to this form of Hb, its reactivity has not been investigated in detail. In this study, we showed that the rate of oxidation of HbFe(II)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0475929
更新日期:2005-04-26 00:00:00
abstract::Previous work from this laboratory has established that the NADPH-menadione oxidoreductase reaction catalyzed by methylenetetrahydrofolate reductase from pig liver proceeds by Ping Pong Bi Bi kinetics and that the reductive half-reaction is rate limiting in steady-state turnover. We have now shown that methylenetetrah...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00317a027
更新日期:1984-10-23 00:00:00
abstract::The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a024
更新日期:1993-04-13 00:00:00
abstract::To test whether the Y-shaped conformation of the hammerhead ribozyme is maintained throughout the catalytic pathway, the cleavage properties of circular substrates which bind the ribozyme through helices I and II were determined. Constraining the position of helices I and II in this manner did not significantly alter ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980307z
更新日期:1998-06-30 00:00:00
abstract::We found that recombinant human adult hemoglobin (rHb A) expressed in Escherichia coli showed heterogeneity of components with the intensity of a positive CD band at 260 nm and that it could be resolved into three components (SP-1, SP-2, and SP-3) by SP-Sepharose column chromatography. 1H NMR revealed that SP-1 is ide...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7015519
更新日期:2008-01-15 00:00:00
abstract::By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein bio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020352d
更新日期:2002-09-24 00:00:00
abstract::Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0607352
更新日期:2006-08-08 00:00:00
abstract::The prothrombinase complex assembles through reversible interactions between factor Xa, factor Va and acidic phospholipid-containing membranes in the presence of calcium ions. This complex catalyses the conversion of prothrombin to thrombin through two proteolytic steps. We have used prethrombin 2 as a substrate analo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9623993
更新日期:1997-03-18 00:00:00
abstract::Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3014642
更新日期:2013-02-12 00:00:00
abstract::The marked difference between the intra- and extracellular 31P NMR chemical shifts of various phosphoryl compounds when added to a red cell suspension may be largely understood in terms of the effects of hemoglobin on the 31P NMR chemical shifts. The presence of [oxy- or (carbonmonoxy)-] hemoglobin inside the red cell...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00424a017
更新日期:1988-11-29 00:00:00
abstract::The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00246a003
更新日期:1991-08-13 00:00:00
abstract::The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the proteas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060451h
更新日期:2006-09-05 00:00:00
abstract::Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00060a030
更新日期:1993-03-09 00:00:00
abstract::Carboxylation of vitamin K-dependent (VKD) proteins is required for their activity and depends on reduced vitamin K generated by vitamin K oxidoreductase (VKOR) and a redox protein that regenerates VKOR activity. VKD protein carboxylation is inefficient in mammalian cells, and to understand why carboxylation becomes s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051986y
更新日期:2006-05-02 00:00:00
abstract::The aureolic acid antitumor antibiotic mithramycin (MTM) inhibits both cancer growth and bone resorption by cross-linking GC-rich DNA, thus blocking binding of Sp-family transcription factors to gene regulatory elements. Transcription of c-src, a gene implicated in many human cancers and required for osteoclast-depend...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034091z
更新日期:2003-07-15 00:00:00
abstract::The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00939
更新日期:2019-04-30 00:00:00
abstract::Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00514a019
更新日期:1981-05-26 00:00:00
abstract::Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a007
更新日期:1979-09-18 00:00:00
abstract::Protein-protein interactions mediate the vast majority of cellular processes. Though protein interactions obey basic chemical principles also within the cell, the in vivo physiological environment may not allow for equilibrium to be reached. Thus, in vitro measured thermodynamic affinity may not provide a complete pic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00172
更新日期:2018-08-07 00:00:00
abstract::Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9828573
更新日期:1999-04-27 00:00:00
abstract::Synthetic nonviral vector systems are attractive because of their apparent simplicity of preparation and use. However, there are many barriers to success at the moment, including the formulation of uniform and reproducible particles of transfection competent, condensed nucleic acids such as plasmid DNA (pDNA). For thi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034325e
更新日期:2003-09-09 00:00:00
abstract::Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a014
更新日期:1977-04-05 00:00:00
abstract::Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9010607
更新日期:2009-09-08 00:00:00
abstract::The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00539a003
更新日期:1982-05-11 00:00:00
abstract::The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001206f
更新日期:2000-10-24 00:00:00
abstract::Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a018
更新日期:1976-10-19 00:00:00
abstract::The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00309a013
更新日期:1984-07-03 00:00:00
abstract::The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201603f
更新日期:2012-01-10 00:00:00
abstract::We use laser flash photolysis and time-resolved Raman spectroscopy of CO-bound H93G myoglobin (Mb) mutants to study the influence of the proximal ligand on the CO rebinding kinetics. In H93G mutants, where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands (e.g., imidazole, 4-b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049077g
更新日期:2004-08-31 00:00:00
abstract::Aquaporin-4 (AQP4) is the predominant water channel in the brain. Although AQP4 plays an important role in brain water homeostasis, the molecular mechanisms of AQP4 regulation are not fully understood. In this report, we show how Zn(2+) rapidly and reversibly decreases the water permeability of AQP4 when it is reconst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901762y
更新日期:2009-12-29 00:00:00