Investigations of photolysis and rebinding kinetics in myoglobin using proximal ligand replacements.

abstract：:We describe a series of indolequinones as efficient mechanism-based inhibitors of NRH:quinone oxidoreductase 2 (NQO2) for use either in cellular or cell-free systems. Compounds were designed to be reduced in the active site of the enzyme leading to loss of a substituted phenol leaving group and generation of a reactiv...

journal_title：Biochemistry

authors： Yan C,Dufour M,Siegel D,Reigan P,Gomez J,Shieh B,Moody CJ,Ross D

更新日期：2011-08-09 00:00:00

abstract：:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs du...

journal_title：Biochemistry

authors： Ivanetich KM,Bradshaw JJ,Kaminsky LS

更新日期：1976-03-09 00:00:00

abstract：:Apolipoprotein E is immunochemically localized to amyloid plaque in Alzheimer's brains, and the allelic distribution of ApoE in individuals is associated with a disposition toward Alzheimer's disease. We show here that all three ApoE isotypes exhibit a strong and specific ability to inhibit both nucleation and seeding...

journal_title：Biochemistry

authors： Wood SJ,Chan W,Wetzel R

更新日期：1996-09-24 00:00:00

abstract：:A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...

journal_title：Biochemistry

authors： Springs SL,Bass SE,McLendon GL

更新日期：2000-05-23 00:00:00

abstract：:The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...

journal_title：Biochemistry

authors： Kodali DR,Fahey DA,Small DM

更新日期：1990-12-04 00:00:00

abstract：:By following the kinetics of abortive and productive synthesis in single-round transcription assays, we confirm the existence of two general classes of initial transcribing complexes (ITCs), which we term "productive ITC" and "unproductive ITC". The productive ITCs are able to escape from the promoter rapidly to produ...

journal_title：Biochemistry

authors： Vo NV,Hsu LM,Kane CM,Chamberlin MJ

更新日期：2003-04-08 00:00:00

abstract：:In the ATP-dependent carboxylation of biotin that is catalyzed by most biotin-dependent carboxylases, a fundamental mechanistic question is whether the ATP activates bicarbonate (via the formation of carboxyphosphate as an intermediate) or whether the ATP activates biotin (via the formation of O-phosphobiotin). We hav...

journal_title：Biochemistry

authors： Ogita T,Knowles JR

更新日期：1988-10-18 00:00:00

abstract：:The solution structures of isolated monomeric actins in their Mg(2+)-ATP and Ca(2+)-ATP bound forms and in complexes with gelsolin segment-1 have been probed using hydroxyl radicals (*OH) generated by synchrotron X-ray radiolysis. Proteolysis and mass spectrometry analysis of 28 peptides containing 58 distinct reactiv...

journal_title：Biochemistry

authors： Guan JQ,Almo SC,Reisler E,Chance MR

更新日期：2003-10-21 00:00:00

abstract：:Sphingosine, sphinganine, and other long-chain (sphingoid) bases inhibit protein kinase C in vitro and block cellular responses to agonists that are thought to act via this enzyme. To gain further insight into the mechanism of this inhibition, a series of long-chain analogues differing in alkyl chain length (11-20 car...

journal_title：Biochemistry

authors： Merrill AH Jr,Nimkar S,Menaldino D,Hannun YA,Loomis C,Bell RM,Tyagi SR,Lambeth JD,Stevens VL,Hunter R

更新日期：1989-04-18 00:00:00

abstract：:The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...

journal_title：Biochemistry

authors： Ramsey AJ,Fitzpatrick PF

更新日期：1998-06-23 00:00:00

abstract：:Three unfolding domains in rabbit muscle aldolase destabilized in 3 M urea have been identified from their unfolding rate constants (0.10, 0.036, and 0.0064 min-1). The populations of folded and various, partially unfolded forms were determined by amide hydrogen exchange and mass spectrometry. Results of this study sh...

journal_title：Biochemistry

authors： Deng Y,Smith DL

更新日期：1998-05-05 00:00:00

abstract：:Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...

journal_title：Biochemistry

authors： Milne SB,Ivanova PT,Armstrong MD,Myers DS,Lubarda J,Shulga YV,Topham MK,Brown HA,Epand RM

更新日期：2008-09-09 00:00:00

abstract：:Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...

journal_title：Biochemistry

authors： Moraitis MI,Xu H,Matthews KS

更新日期：2001-07-10 00:00:00

abstract：:The dual specificity mitogen-activated protein kinase phosphatase MKP3 downregulates mitogenic signaling through dephosphorylation of extracellular signal-regulated kinase (ERK). Like other MKPs, MKP3 consists of a noncatalytic N-terminal domain and a catalytic C-terminal domain. ERK binding to the N-terminal noncatal...

journal_title：Biochemistry

authors： Kim Y,Rice AE,Denu JM

更新日期：2003-12-30 00:00:00

abstract：:We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...

journal_title：Biochemistry

authors： Jennings ML,Nicknish JS

更新日期：1984-12-18 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

journal_title：Biochemistry

authors： Eckl JM,Drazic A,Rutz DA,Richter K

更新日期：2014-04-22 00:00:00

abstract：:Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and map...

journal_title：Biochemistry

authors： Bae SH,Liu D,Lim HM,Lee Y,Choi BS

更新日期：2008-02-19 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monomers, and related enzyme domains embedded within inteins. DNA-bound structures of homodimeric I-CreI and monomeric I-SceI indicate that three catalytic divalent metal ions are distributed across a pair of overlapping active sites,...

journal_title：Biochemistry

authors： Chevalier B,Sussman D,Otis C,Noël AJ,Turmel M,Lemieux C,Stephens K,Monnat RJ Jr,Stoddard BL

更新日期：2004-11-09 00:00:00

abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...

journal_title：Biochemistry

authors： Kellenberger C,Hendrickson TL,Imperiali B

更新日期：1997-10-14 00:00:00

abstract：:Intracellular reduction of carcinogenic Cr(VI) generates Cr-DNA adducts formed through the coordination of Cr(III) to DNA phosphates (phosphotriester-type adduct). Here, we examined the role of Cr(III)-DNA adducts in mutagenesis induced by metabolism of Cr(VI) with cysteine. Reduction of Cr(VI) caused a strong oxidati...

journal_title：Biochemistry

authors： Zhitkovich A,Song Y,Quievryn G,Voitkun V

更新日期：2001-01-16 00:00:00

abstract：:The complete amino acid sequence was determined for bovine ubiquitin, and adenylate cyclase stimulating polypeptide, which is probably represented universally in living cells. Ubiquitin has a molecular weight of 8451 and consists of a single polypeptide chain containing 74 amino acid residues. It contains four argini...

journal_title：Biochemistry

authors： Schlesinger DH,Goldstein G,Niall HD

更新日期：1975-05-20 00:00:00

abstract：:The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...

journal_title：Biochemistry

authors： Momb J,Wang C,Liu D,Thomas PW,Petsko GA,Guo H,Ringe D,Fast W

更新日期：2008-07-22 00:00:00

abstract：:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...

journal_title：Biochemistry

authors： Thompson RE,Reynolds HY,Waxdal MJ

更新日期：1975-07-01 00:00:00

abstract：:Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...

journal_title：Biochemistry

authors： Kucukkal TG,Yang Y,Uvarov O,Cao W,Alexov E

更新日期：2015-10-20 00:00:00

abstract：:The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...

journal_title：Biochemistry

authors： Görisch H,Goss DJ,Parkhurst LJ

更新日期：1976-12-28 00:00:00

abstract：:Cytochrome bc(1), a key enzyme of biological energy conversion, generates or uses a proton motive force through the Q cycle that operates within the two chains of cofactors that embed two catalytic quinone oxidation/reduction sites, the Q(o) site and the Q(i) site. The Q(o) site relies on the joint action of two cofac...

journal_title：Biochemistry

authors： Borek A,Sarewicz M,Osyczka A

更新日期：2008-11-25 00:00:00

abstract：:Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...

journal_title：Biochemistry

authors： Shanmugasundaram T,Kumar GK,Wood HG

更新日期：1988-08-23 00:00:00

abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

journal_title：Biochemistry

authors： Kuo W,Herrick DZ,Cafiso DS

更新日期：2011-04-05 00:00:00

abstract：:Seven hydrophobic residues ranging in size from glycine to phenylalanine have been substituted for the wild-type methionine residue at position 13 in a 15-residue truncated version (S15) of S-peptide, the small component of ribonuclease S. Complexes of both S-15 and the seven variants with S-protein yielded isomorphou...

journal_title：Biochemistry

authors： Varadarajan R,Richards FM

更新日期：1992-12-15 00:00:00