Abstract:
:Aquaporin-4 (AQP4) is the predominant water channel in the brain. Although AQP4 plays an important role in brain water homeostasis, the molecular mechanisms of AQP4 regulation are not fully understood. In this report, we show how Zn(2+) rapidly and reversibly decreases the water permeability of AQP4 when it is reconstituted into proteoliposomes. Mutagenesis analysis identified Cys178, located in cytoplasmic loop D, as a target residue of ZnCl(2) inhibition. Moreover, treatment with diamide enhanced the inhibitory effects of ZnCl(2). These results suggest that the water permeability of AQP4 may be regulated by dynamic changes in intracellular Zn(2+) concentration linked to the cellular redox state.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yukutake Y,Hirano Y,Suematsu M,Yasui Mdoi
10.1021/bi901762ysubject
Has Abstractpub_date
2009-12-29 00:00:00pages
12059-61issue
51eissn
0006-2960issn
1520-4995journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3000462
更新日期:2012-06-26 00:00:00
abstract::The Escherichia coli repressor of biotin biosynthesis is both a biotin ligase and the repressor of transcriptional initiation at the biotin biosynthetic operon. The small molecule, biotinyl-5'-adenylate (bio-5'-AMP), is the intermediate in the biotin ligation reaction and the positive allosteric effector for sequence-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a041
更新日期:1994-06-14 00:00:00
abstract::The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00426a014
更新日期:1988-12-27 00:00:00
abstract::Many drugs are oxidized by membrane protein cytochrome P450 (CYP) enzymes during their metabolism process. CYPs are located mainly in endoplasmic reticulum (ER) membranes. Recent studies have suggested that CYP substrate drugs first bind the lipid bilayers of ER membranes and then the drugs reach the active site of CY...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00286
更新日期:2016-07-19 00:00:00
abstract::The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00677a035
更新日期:1975-03-25 00:00:00
abstract::Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi300752r
更新日期:2012-07-24 00:00:00
abstract::Despite significant progress in the past decade, questions still remain about the complete structural, dynamic, and thermodynamic effect of the cis-syn cyclobutane pyrimidine dimer lesion (hereafter called the thymine dimer) on double-stranded genomic DNA. We examined a 19-mer oligodeoxynucleotide duplex containing a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801417u
更新日期:2008-12-09 00:00:00
abstract::Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00250a022
更新日期:1994-11-22 00:00:00
abstract::The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036338y
更新日期:2004-03-30 00:00:00
abstract::BtuB is a TonB-dependent transport protein that binds and carries vitamin B(12) across the outer membrane of Gram negative bacteria such as Escherichia coli. Previous work has demonstrated that the Ton box, a highly conserved segment near the N-terminus of the protein, undergoes an order-to-disorder transition upon th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061051x
更新日期:2006-09-12 00:00:00
abstract::Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e.,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00585a004
更新日期:1979-09-04 00:00:00
abstract::Lactyl-CoA dehydratase consists of two enzymes, E1 and E2, and requires catalytic quantities of ATP for activity [Kuchta, R. D., & Abeles, R. H. (1985) J. Biol. Chem. 260, 13181-13189]. In contrast to E1, which contains no Fe, E2 contains 8.20 +/- 0.04 mol of Fe/mol of E2, one of which can be removed by 1,10-phenanthr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a009
更新日期:1986-11-18 00:00:00
abstract::Disruption of the cell membrane by the β-amyloid (Aβ) peptides has been considered as a main mechanism of Alzheimer's disease. The peptide-to-lipid molar ratio (P:L) varies over a broad range biologically. We report here that two of the previously observed Aβ evolution pathways, fibrillation and induction of vesicle f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00321
更新日期:2015-06-09 00:00:00
abstract::Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7009508
更新日期:2007-09-04 00:00:00
abstract::The cyanobacterium Synechocystis sp. PCC 6803 (S6803) expresses a two-on-two globin in which His46 (distal side) and His70 (proximal) function as heme iron axial ligands. His46 can be displaced by O2, CO, and CN-, among others, whereas His70 is not labile under native conditions. The residue preceding the proximal his...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060691x
更新日期:2006-09-26 00:00:00
abstract::Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200598r
更新日期:2011-08-23 00:00:00
abstract::Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981743o
更新日期:1999-02-02 00:00:00
abstract::The precise nature of redox modulation of N-methyl-d-aspartate (NMDA) receptors is still unclear, although it is thought to be related to the formation and breaking of disulfide bonds. Recent structural data demonstrated the way in which disulfide bonds in the ligand-binding core of the NR1 subunit are arranged. Howev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061462d
更新日期:2007-02-27 00:00:00
abstract::The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011173j
更新日期:2001-10-02 00:00:00
abstract::Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniq...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00596a020
更新日期:1978-02-07 00:00:00
abstract::Site-specific mutagenesis has been used to create two mutant versions of aspartate transcarbamoylase. Arg-167 and Gln-231, both previously identified as interacting with the portion of the bisubstrate analogue N-(phosphonoacetyl)-L-aspartate (PALA) that corresponds to aspartate [Krause, K. L., Voltz, K. W., & Lipscomb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00468a003
更新日期:1990-04-24 00:00:00
abstract::In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801831r
更新日期:2009-02-03 00:00:00
abstract::Four mechanisms for the allosteric regulation of the calcium and magnesium ion activated adenosinetriphosphatase (Ca,Mg-ATPase) of sarcoplasmic reticulum were examined. Negative cooperativity in substrate binding was not supported by 3H-labeled 5'-adenylyl methylenediphosphate (AMPPCP) binding, which was best fit by a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a049
更新日期:1985-09-24 00:00:00
abstract::Carbonic anhydrase (CA) catalyzes the reversible hydration of carbon dioxide to hydrogen carbonate, and its role in maintaining pH balance has made it an attractive drug target. Steroidal sulfamate esters, inhibitors of the cancer drug target steroid sulfatase (STS), are sequestered in vivo by CA II in red blood cells...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902178w
更新日期:2010-04-27 00:00:00
abstract::Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01033
更新日期:2019-01-15 00:00:00
abstract::Escherichia coli alkaline phosphatase (AP) is the prototypical two metal ion catalyst with two divalent zinc ions bound approximately 4 A apart in the active site. Studies spanning half a century have elucidated many structural and mechanistic features of this enzyme, rendering it an attractive model for investigating...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012166y
更新日期:2002-03-05 00:00:00
abstract::Nitric oxide synthases (NOSs) are proposed to generate NO and citrulline from L-arginine in two steps: initial N-hydroxylation to generate Nomega-hydroxyarginine (NOHA) followed by a three-electron oxidation of the hydroxylated nitrogen to form products. Both steps consume NADPH and may involve heme iron-based activat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971414g
更新日期:1997-09-09 00:00:00
abstract::Subunit a of the Escherichia coli ATP synthase is thought to control access of protons to the ring of c subunits during proton-driven ATP synthesis. In this study, the surface exposure of subunit a in the periplasm has been examined using 3-N-maleimidyl-propionyl biocytin labeling in cells permeabilized by polymyxin B...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026649t
更新日期:2003-01-21 00:00:00
abstract::Peptide-membrane interactions are important for understanding the binding, partitioning, and folding of membrane proteins; the activity of antimicrobial and fusion peptides; and a number of other processes. We describe molecular dynamics simulations (10-25 ns) of two pentapeptides Ace-WLXLL (with X = Arg or Lys side c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027001j
更新日期:2003-08-05 00:00:00
abstract::Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000971r
更新日期:2000-10-10 00:00:00