Effect of the delta subunit of Bacillus subtilis RNA polymerase on initiation of RNA synthesis at two bacteriophage phi 29 promoters.

abstract：:A mouse monoclonal IgG1 antibody has been generated against the soluble form of the calmodulin-dependent protein kinase type II. This antibody recognizes both the soluble and cytoskeletal forms of the enzyme, requiring Ca2+ (EC50 = 20 microM) for the interaction. Other divalent cations such as Zn2+, Mn2+, Cd2+, Co2+, ...

journal_title：Biochemistry

authors： LeVine H 3rd,Su JL,Sahyoun NE

更新日期：1988-08-23 00:00:00

abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...

journal_title：Biochemistry

authors： Whitty A,Fierke CA,Jencks WP

更新日期：1995-09-19 00:00:00

abstract：:The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...

journal_title：Biochemistry

authors： Ashkenazi A,Merklinger E,Shai Y

更新日期：2012-09-04 00:00:00

abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...

journal_title：Biochemistry

authors： An S,Yang J,So SW,Zeng L,Goetzl EJ

更新日期：1994-12-06 00:00:00

abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...

journal_title：Biochemistry

authors： Sich C,Ohlenschläger O,Ramachandran R,Görlach M,Brown LR

更新日期：1997-11-18 00:00:00

abstract：:In Bacillus subtilis, P protein is the noncatalytic component of ribonuclease P (RNase P) that is critical for achieving maximal nuclease activity under physiological conditions. P protein is predominantly unfolded (D) at neutral pH and low ionic strength; however, it folds upon the addition of sulfate anions (ligands...

journal_title：Biochemistry

authors： Henkels CH,Oas TG

更新日期：2005-10-04 00:00:00

abstract：:Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ternary complexes influence ET. We probe this issue through a study of the photocycle...

journal_title：Biochemistry

authors： Page TR,Hoffman BM

更新日期：2015-02-10 00:00:00

abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...

journal_title：Biochemistry

authors： House CH,Miller SL

更新日期：1996-01-09 00:00:00

abstract：:To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...

journal_title：Biochemistry

authors： Hausmann NZ,Znosko BM

更新日期：2012-07-03 00:00:00

abstract：:Group I intron RNAs contain a core of highly conserved helices flanked by peripheral domains that stabilize the core structure. In the Tetrahymena group I ribozyme, the P4, P5, and P6 helices of the core pack tightly against a three-helix subdomain called P5abc. Chemical footprinting and the crystal structure of the T...

journal_title：Biochemistry

authors： Doherty EA,Herschlag D,Doudna JA

更新日期：1999-03-09 00:00:00

abstract：:Fibroblast growth factors (FGFs) are mitogenic and chemotactic agents for a wide variety of cell types and play a primary role in the regulation of angiogenesis. Angiogenesis is involved in a variety of critical physiological events including organogenesis, wound healing, ischemic collateral circulation, and solid tum...

journal_title：Biochemistry

authors： Blaber M,DiSalvo J,Thomas KA

更新日期：1996-02-20 00:00:00

abstract：:The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...

journal_title：Biochemistry

authors： Chelsky D,Dahlquist FW

更新日期：1981-02-17 00:00:00

abstract：:4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a member of the xanthine oxidase (XO) family of molybdenum cofactor containing enzymes and catalyzes the irreversible removal of a phenolic hydroxy group by reduction, yielding benzoyl-CoA and water. In this work the effects of various activity modulating compounds were chara...

journal_title：Biochemistry

authors： Johannes J,Unciuleac MC,Friedrich T,Warkentin E,Ermler U,Boll M

更新日期：2008-04-29 00:00:00

abstract：:We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...

journal_title：Biochemistry

authors： Boles TC,Hogan ME

更新日期：1986-05-20 00:00:00

abstract：:The operator-binding domain of phage lambda repressor provides a model for DNA recognition by the helix-turn-helix (HTH) motif. In the wild-type protein, dimerization is mediated by hydrophobic packing (of the dyad-related helix 5), which serves as an indirect determinant of operator affinity. The mutant repressor, Ty...

journal_title：Biochemistry

authors： Benevides JM,Weiss MA,Thomas GJ Jr

更新日期：1991-05-07 00:00:00

abstract：:Rat alpha- and beta-parvalbumins have distinct monovalent cation-binding properties [Henzl et al. (2000) Biochemistry 39, 5859-5867]. Beta binds two Na(+) or one K(+), and alpha binds one Na(+) and no K(+). Ca(2+) abolishes these binding events, suggesting that the monovalent ions occupy the EF-hand motifs. This study...

journal_title：Biochemistry

authors： Henzl MT,Larson JD,Agah S

更新日期：2004-03-16 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:Chemical modification of Torpedo californica acetylcholinesterase by various sulfhydryl reagents results in its conversion to one of two principal states. One of these states, viz., that produced by disulfides and alkylating agents, is stable. The second state, produced by mercury derivatives, is metastable. At room t...

journal_title：Biochemistry

authors： Kreimer DI,Dolginova EA,Raves M,Sussman JL,Silman I,Weiner L

更新日期：1994-12-06 00:00:00

abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

journal_title：Biochemistry

authors： Jones DH,Barber KR,Grant CW

更新日期：1996-04-16 00:00:00

abstract：:Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA by addition of a nucleotide to an electrophilic cyclop...

journal_title：Biochemistry

authors： Xue M,Shine E,Wang W,Crawford JM,Herzon SB

更新日期：2018-11-13 00:00:00

abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...

journal_title：Biochemistry

authors： Schwehm JM,Kristyanne ES,Biggers CC,Stites WE

更新日期：1998-05-12 00:00:00

abstract：:The stability and folding kinetics of wild-type and a mutant staphylococcal nuclease (SNase) at neutral pH are significantly perturbed by the presence of moderate to high concentrations of salts. Very substantial increases in stability toward thermal and urea denaturation were observed; for example, 0.4 M sodium sulfa...

journal_title：Biochemistry

authors： Nishimura C,Uversky VN,Fink AL

更新日期：2001-02-20 00:00:00

abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...

journal_title：Biochemistry

authors： Zhang L,Wu J,Ruan KH

更新日期：2006-02-14 00:00:00

abstract：:The preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid I, are described. CO association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped...

journal_title：Biochemistry

authors： Goss DJ,Parkhurst LJ

更新日期：1984-05-08 00:00:00

abstract：:The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

journal_title：Biochemistry

authors： Betlach MC,Kealey JT,Ashley GW,McDaniel R

更新日期：1998-10-20 00:00:00

abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...

journal_title：Biochemistry

authors： Childers MC,Daggett V

更新日期：2019-11-05 00:00:00

abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...

journal_title：Biochemistry

authors： Piersma SR,Visser AJ,de Vries S,Duine JA

更新日期：1998-03-03 00:00:00

abstract：:F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...

journal_title：Biochemistry

authors： Bouvier D,Vanhaverbeke C,Simorre JP,Arlaud GJ,Bally I,Forge V,Margolis RL,Gans P,Kleman JP

更新日期：2003-10-07 00:00:00

abstract：:One of the most intriguing questions confronting the bone morphogenetic protein family is the mechanism of ligand recognition, because there are more ligands than receptors. Crystal structures of two type II receptors, ActR-II and BMPR-II, are essentially identical, and a loop structure (A-loop) has been suggested to ...

journal_title：Biochemistry

authors： Yeh LC,Falcon WE,Garces A,Lee JC,Lee JC

更新日期：2012-09-04 00:00:00

abstract：:The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thal...

journal_title：Biochemistry

authors： Edvardsson A,Shapiguzov A,Petersson UA,Schröder WP,Vener AV

更新日期：2007-08-21 00:00:00