Abstract：
:The effect of Ca2+ on the interaction of bovine cardiac myosin subfragment 1 (S-1) with actin regulated by cardiac troponin-tropomyosin was evaluated. The ratios of actin to troponin and to tropomyosin were adjusted to optimize the Ca2+-dependent regulation of the steady-state actin-activated magnesium adenosinetriphosphatase (MgATPase) rate of myosin S-1. At 25 degrees C, pH 6.9, 16 mM ionic strength, the extrapolated values for maximal adenosine 5'-triphosphate (ATP) turnover rate at saturating actin, Vmax, were 6.5 s-1 in the presence of Ca2+ and 0.24 s-1 in the absence of Ca2+. In contrast to this 27-fold regulation of ATP hydrolysis, there was negligible Ca2+-dependent regulation of cardiac myosin S-1 binding to actin. In the presence of ATP, the dissociation constant of regulated actin and cardiac myosin S-1 was 32 microM in the presence of Ca2+ and 40 microM in the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid. These dissociation constants are indistinguishable from the concentrations of actin needed to reach half-saturation of the myosin S-1 MgATPase rates, 37 microM actin in the presence of Ca2+ and 53 microM in its absence. Although there may be Ca2+-dependent regulation of cross-bridge binding in the intact heart, the present biochemical studies suggest that cardiac regulation critically involves other parts of the cross-bridge cycle, evidenced here by almost complete Ca2+-mediated control of the myosin S-1 MgATPase rate even when the myosin S-1 is actin-bound.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tobacman LS,Adelstein RSdoi
10.1021/bi00352a010subject
Has Abstractpub_date
1986-02-25 00:00:00pages
798-802issue
4eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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