Abstract：
:The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show that two relatively small fragments of a1 and alpha 2 are capable of heterodimerization and tight DNA binding. The alpha 2 fragment contains the homeodomain followed by the natural 22 C-terminal amino acids of the protein; these 22 amino acids are unstructured in the alpha 2 fragment. The a1 fragment contains only the homeodomain, indicating that the a1 homeodomain mediates both DNA binding and protein-protein interactions with alpha 2. We used isotope-edited NMR spectroscopy to study the interaction in solution of these two fragments. Samples in which only the alpha 2 fragment was uniformly labeled with 15N allowed us to visualize changes in the NMR spectra of the alpha 2 fragment produced by heterodimerization. We found that the a1 homeodomain perturbs the resonances of only the C-terminal tail of alpha 2; moreover, contact with a1 converts a portion of this tail (residues 193-203) from its unstructured state to an alpha-helix, as determined by J coupling and NOE measurements. Thus the heterodimerization of two homeodomain proteins involves the specific interaction between a tail of one protein and the homeodomain of the other. This interaction is accompanied by the acquisition of secondary structure in the tail.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Phillips CL,Stark MR,Johnson AD,Dahlquist FWdoi
10.1021/bi00197a033subject
Has Abstractpub_date
1994-08-09 00:00:00pages
9294-302issue
31eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The structure of MtrA, an essential gene product for the human pathogen Mycobacterium tuberculosis, has been solved to a resolution of 2.1 A. MtrA is a member of the OmpR/PhoB family of response regulators and represents the fourth family member for which a structure of the protein in its inactive state has been deter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602546q
更新日期：2007-06-12 00:00:00
abstract：:The unfolding and dissociation of the tetrameric enzyme fructose-1,6-bisphosphatase from pig kidney by guanidine hydrochloride have been investigated at equilibrium by monitoring enzyme activity, ANS binding, intrinsic (tyrosine) protein fluorescence, exposure of thiol groups, fluorescence of extrinsic probes (AEDANS,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034203m
更新日期：2003-06-17 00:00:00
abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00533a030
更新日期：1982-02-16 00:00:00
abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300295y
更新日期：2012-04-24 00:00:00
abstract：:We have used frequency domain fluorescence techniques to resolve the component emission spectra for several two tryptophan containing proteins (e.g., horse liver alcohol dehydrogenase, sperm whale apomyoglobin, yeast 3-phosphoglycerate kinase, apoazurin from Alcaligenes denitricans). We have first performed multifrequ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00399a047
更新日期：1987-12-15 00:00:00
abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a043
更新日期：1980-07-08 00:00:00
abstract：:When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a rib...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036290o
更新日期：2004-05-25 00:00:00
abstract：:Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00417a016
更新日期：1988-08-23 00:00:00
abstract：:The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700406k
更新日期：2007-09-18 00:00:00
abstract：:hOgg1 and FPG are the primary DNA repair enzymes responsible for removing the major guanine (G) oxidative product, namely, 7,8-dihydro-8-oxoguanine (OG), in humans and bacteria, respectively. While natural G adopts the anti conformation and forms a Watson-Crick pair with cytosine (C), OG can also adopt the syn conform...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01292
更新日期：2018-02-20 00:00:00
abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9621674
更新日期：1997-02-25 00:00:00
abstract：:As a light-driven water-plastoquinone oxidoreductase, Photosystem II produces molecular oxygen as an enzymatic product. Additionally, under a variety of stress conditions, reactive oxygen species are produced at or near the active site for oxygen evolution. In this study, Fourier-transform ion cyclotron resonance mass...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300650n
更新日期：2012-08-14 00:00:00
abstract：:Calf brain tubulin was subjected to isoelectric focusing and tryptic peptide map analysis. Results from isoelectric focusing experiments showed a total number of 17 well-resolved protein peaks. The number of peaks and the mass distribution under each peak remained the same when the concentration of protein or ampholyt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00512a006
更新日期：1981-04-28 00:00:00
abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972723+
更新日期：1998-02-03 00:00:00
abstract：:The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00461a014
更新日期：1990-03-06 00:00:00
abstract：:A method has been developed for the routine synthesis of 2'(3')-o-monoacyl ribonucleoside 5'-diphosphates for stepwise synthesis of oligoribonucleotides with Escherichia coli polynucleotide phosphorylase. The use of triethyl orthoisovalerate allows the facile preparation of 2'(3')-o-isovaleryl-UDP, -CDP, -ADP, -GDP, -...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00675a027
更新日期：1975-02-25 00:00:00
abstract：:Coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress. While PARP-1 and -2 are regulated by DNA breaks and covalent modifications, mechanisms of PARG regulation are poorly understood. We report here discovery of a PARG regulatory segment far removed linearly from residu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100973m
更新日期：2010-09-07 00:00:00
abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00285a008
更新日期：1983-08-02 00:00:00
abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061632m
更新日期：2006-12-26 00:00:00
abstract：:The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963018o
更新日期：1997-07-08 00:00:00
abstract：:His12 and His119 are critical for catalysis of RNA cleavage by ribonuclease A (RNase A). Substitution of either residue with an alanine decreases the value of k(cat)/K(M) by more than 10(4)-fold. His12 and His119 are proximal to the scissile phosphoryl group of an RNA substrate in enzyme-substrate complexes. Here, the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0100182
更新日期：2001-04-24 00:00:00
abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a015
更新日期：1993-10-12 00:00:00
abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00796
更新日期：2021-01-13 00:00:00
abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400053s
更新日期：2013-03-12 00:00:00
abstract：:The third intracellular loop (IL3) of G protein-coupled receptors (GPCRs) is an important contact domain between GPCRs and their G proteins. Previously, the IL3 of Ste2p, a Saccharomyces cerevisiae GPCR, was suggested to undergo a conformational change upon activation as detected by differential protease susceptibilit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200254h
更新日期：2011-08-16 00:00:00
abstract：:The glycolytic enzyme fructose-1,6-bisphosphate aldolase (FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Catalysis of Schiff base forming class I FBPA relies on a number of intermediates covalently bound to the catalytic lysine. Using ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048192o
更新日期：2005-03-22 00:00:00
abstract：:The kcat and Km values for the bovine heart low molecular weight phosphotyrosyl protein phosphatase catalyzed hydrolysis of 16 aryl phosphate monoesters and of five alkyl phosphate monoesters having the structure Ar(CH2)nOPO3H2 (n = 1-5) were measured at pH 5.0 and 37 degrees C. With the exception of alpha-naphthyl ph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00101a006
更新日期：1991-09-17 00:00:00
abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00619a034
更新日期：1978-12-26 00:00:00
abstract：:Murine fibroblasts cultured in the presence of fibroblast growth factor-1 express relatively high levels of FR-1, a approximately 36 kDa protein related to the aldo-keto reductase superfamily [Donohue, P. J., Alberts, G. F., Hampton, B. S., Winkles, J. A. (1994) J. Biol. Chem. 269, 8604-8609]. While the crystal struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9804333
更新日期：1998-09-15 00:00:00
abstract：:For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0255322
更新日期：2002-04-09 00:00:00
