Abstract:
:As a light-driven water-plastoquinone oxidoreductase, Photosystem II produces molecular oxygen as an enzymatic product. Additionally, under a variety of stress conditions, reactive oxygen species are produced at or near the active site for oxygen evolution. In this study, Fourier-transform ion cyclotron resonance mass spectrometry was used to identify oxidized amino acid residues located in several core Photosystem II proteins (D1, D2, CP43, and CP47) isolated from spinach Photosystem II membranes. While the majority of these oxidized residues (81%) are located on the oxygenated solvent-exposed surface of the complex, several residues on the CP43 protein ((354)E, (355)T, (356)M, and (357)R) which are in close proximity (<15 Å) to the Mn(4)CaO(5) active site are also modified. These residues appear to be associated with putative oxygen/reactive oxygen species exit channel(s) in the photosystem. These results are discussed within the context of a number of computational studies which have identified putative oxygen channels within the photosystem.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Frankel LK,Sallans L,Limbach PA,Bricker TMdoi
10.1021/bi300650nsubject
Has Abstractpub_date
2012-08-14 00:00:00pages
6371-7issue
32eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The 70-kDa subunit of eukaryotic replication protein A (RPA) contains a conserved four cysteine-type zinc-finger motif that has been implicated in regulation of DNA replication and repair. Unlike other zinc-finger proteins, RPA zinc-finger motif is not a DNA-binding component, and deletion of the zinc-finger had very ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001206f
更新日期:2000-10-24 00:00:00
abstract::Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00335a030
更新日期:1985-07-02 00:00:00
abstract::The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00120a020
更新日期:1992-02-11 00:00:00
abstract::Decapping is the first committed step in 5'-to-3' RNA decay, and in the cytoplasm of human cells, multiple decapping enzymes regulate the stabilities of distinct subsets of cellular transcripts. However, the complete set of RNAs regulated by any individual decapping enzyme remains incompletely mapped, and no consensus...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00069
更新日期:2020-11-03 00:00:00
abstract::The removal of damaged bases by DNA glycosylases is thought to be effectively irreversible, because of an overall equilibrium that favors hydrolysis over synthesis of the N-glycosyl bond. Surprisingly, human alkyladenine DNA glycosylase (AAG) can make damaged DNA by catalyzing formation of an N-glycosyl bond between 1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101380d
更新日期:2010-10-26 00:00:00
abstract::Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800492c
更新日期:2008-09-09 00:00:00
abstract::Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011885+
更新日期:2002-03-26 00:00:00
abstract::Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a021
更新日期:1995-04-11 00:00:00
abstract::A wide variety of biological processes including differentiation, regeneration, and cancer progression are regulated by shedding of membrane-anchored proteins. One of the major sheddases is A Disintegrin And Metalloprotease-17 (ADAM17) whose extracellular region consists of a pro-, a catalytic, a disintegrin-, and a m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00497
更新日期:2015-09-29 00:00:00
abstract::We have characterized the kinetics and substrate requirements of prenyl-flavin synthase from yeast. This enzyme catalyzes the addition of an isopentenyl unit to reduced flavin mononucleotide (FMN) to form an additional six-membered ring that bridges N5 and C6 of the flavin nucleus, thereby converting the flavin from a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01131
更新日期:2018-02-06 00:00:00
abstract::During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00824
更新日期:2018-04-03 00:00:00
abstract::Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300039f
更新日期:2012-04-10 00:00:00
abstract::Extracellular cAMP induces the rapid activation of guanylate cyclase, which adapts within 10 s to constant cAMP concentrations. A new response can be induced either by a higher cAMP concentration or by the same cAMP concentration at some time (t1/2 = 90 s) after removal of the previous stimulus. Stimulation of guanyla...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a047
更新日期:1987-11-17 00:00:00
abstract::Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300542g
更新日期:2012-09-04 00:00:00
abstract::S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00111a006
更新日期:1991-11-26 00:00:00
abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005528
更新日期:2013-08-06 00:00:00
abstract::For screening a pool of potential substrates that load carrier domains found in nonribosomal peptide synthetases, large molecule mass spectrometry is shown to be a new, unbiased assay. Combining the high resolving power of Fourier transform mass spectrometry with the ability of adenylation domains to select their own ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052333k
更新日期:2006-02-14 00:00:00
abstract::The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici has been solved using X-ray diffraction data extending to atomic resolution, 0.94 A, recorded at 100 K. The model was refined with anisotropic representation of atomic displacement parameters for all non-hydrogen atoms and with hydrogens ridi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972155y
更新日期:1997-12-23 00:00:00
abstract::Using lattice statistical mechanics, we develop theory to account for the folding of a heteropolymer molecule such as a protein to the globular and soluble state. Folding is assumed to be driven by the association of solvophobic monomers to avoid solvent and opposed by the chain configurational entropy. Theory predict...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00327a032
更新日期:1985-03-12 00:00:00
abstract::The catalytic (C) subunit of cAMP-dependent protein kinase interacts with two classes of inhibitors. The regulatory (R) subunits, types I and II, associate to form an inactive holoenzyme complex that is activated in response to cAMP. The C-subunit is also inhibited by small heat-stable protein kinase inhibitors (PKI's...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00213a035
更新日期:1993-12-21 00:00:00
abstract::Discoidal high-density lipoprotein (HDL) particles are known to be fractionalized into several discrete populations in plasma and to differ in behavior according to size; however, their structural differences and the factors regulating their size are less understood. In this study, we prepared several reconstituted HD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900785x
更新日期:2009-08-18 00:00:00
abstract::Many proteins utilize segmental motions to catalyze a specific reaction. The Omega loop of triosephosphate isomerase (TIM) is important for preventing the loss of the reactive enediol(ate) intermediate. The loop opens and closes even in the absence of the ligand, and the loop itself does not change conformation during...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049208d
更新日期:2004-09-14 00:00:00
abstract::Barbiturates inhibit GLUT-1 mediated hexose transport both in vivo [Gjedde & Rasmussen (1980) J. Neurochem. 35, 1382-1387; Otsuka et al. (1991) Am. J. Physiol. 261, R265-R275] and in vitro [Honkanen et al. (1995) Biochemistry 34, 535-544]. In the present study, the mechanism by which barbiturates inhibit GLUT-1 mediat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962050f
更新日期:1996-12-03 00:00:00
abstract::The nucleotide binding site of the uncoupling protein (UCP) from brown adipose tissue was mapped by photoaffinity labeling with 2-azidoadenosine 5'-triphosphate (2-azido-ATP) and by affinity labeling with 3'-O-(5-fluoro-2,4-dinitrophenyl)adenosine 5'-triphosphate (FDNP-ATP). Both analogs bind with high affinity and sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00158a017
更新日期:1992-11-03 00:00:00
abstract::The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00559a003
更新日期:1980-09-02 00:00:00
abstract::Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962355u
更新日期:1997-02-18 00:00:00
abstract::Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a031
更新日期:1995-04-11 00:00:00
abstract::The membrane topology of the human apical sodium-dependent bile acid transporter (hASBT) remains unresolved. Whereas N-glycosylation analysis favors a 7 transmembrane (TM) model, membrane insertion scanning supports a 9TM topology. In order to resolve this controversy, we used dual label epitope insertion to systemati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052202j
更新日期:2006-01-24 00:00:00
abstract::Calmodulin-dependent protein phosphatase, one of the major calmodulin-binding proteins in bovine brain, dephosphorylates casein with a specific activity of 15 nmol mg-1 min-1 at 30 degrees C. The stimulation of phosphatase activity by calmodulin is reversed by ethylene glycol bis (beta-aminoethyl ether)-N,N,N',N'-tetr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00284a014
更新日期:1983-07-19 00:00:00
abstract::A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a011
更新日期:1976-10-19 00:00:00