Abstract:
:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of other CPI-motif protein families. Using biochemical assays with purified proteins, we compared the ability of CPI-motif-containing peptides from different protein families (a) to bind to CP, (b) to allosterically inhibit barbed-end capping by CP, and (c) to allosterically inhibit interaction of CP with V-1, another regulator of CP. We found large differences in potency among the different CPI-motif-containing peptides, and the different functional assays showed different orders of potency. These biochemical differences among the CPI-motif peptides presumably reflect interactions between CP and CPI-motif peptides involving amino acid residues that are conserved but are not part of the strictly defined consensus, as it was originally identified in comparisons of sequences of CPI motifs across all protein families [Hernandez-Valladares, M., et al. (2010) Structural characterization of a capping protein interaction motif defines a family of actin filament regulators. Nat. Struct. Mol. Biol. 17, 497-503; Bruck, S., et al. (2006) Identification of a Novel Inhibitory Actin-capping Protein Binding Motif in CD2-associated Protein. J. Biol. Chem. 281, 19196-19203]. These biochemical differences may be important for conserved distinct functions of CPI-motif protein families in cells with respect to the regulation of CP activity and actin assembly near membranes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
McConnell P,Mekel M,Kozlov AG,Mooren OL,Lohman TM,Cooper JAdoi
10.1021/acs.biochem.0c00092subject
Has Abstractpub_date
2020-03-24 00:00:00pages
1202-1215issue
11eissn
0006-2960issn
1520-4995journal_volume
59pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micromete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200549x
更新日期:2011-05-31 00:00:00
abstract::Folylpoly-gamma-glutamate synthetase (FPGS) is the enzyme responsible for metabolic trapping of reduced folate cofactors in cells for use in nucleotide and amino acid biosynthesis. There are two isoforms of FPGS expressed in mouse tissues, one is expressed in differentiated tissue, principally liver and kidney, and th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015644d
更新日期:2002-01-08 00:00:00
abstract::Threonyl-tRNA synthetase (ThrRS) must discriminate among closely related amino acids to maintain the fidelity of protein synthesis. Here, a pre-steady state kinetic analysis of the ThRS-catalyzed adenylation reaction was carried out by monitoring changes in intrinsic tryptophan fluorescence. Stopped flow fluorimetry f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0355701
更新日期:2003-12-30 00:00:00
abstract::The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00299
更新日期:2020-07-21 00:00:00
abstract::The contribution of backbone hydrogen bonds in alpha-helices to the overall stability of a protein has been examined experimentally by replacing several backbone amide linkages in alpha-helix 39-50 of T4 lysozyme with ester linkages. T4 lysozyme variants wherein the backbone amide bonds between residues Ser38 and Leu3...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9707685
更新日期:1997-09-23 00:00:00
abstract::By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a020
更新日期:1988-05-31 00:00:00
abstract::Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00186a027
更新日期:1994-05-24 00:00:00
abstract::The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011753g
更新日期:2002-02-05 00:00:00
abstract::The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00514a035
更新日期:1981-05-26 00:00:00
abstract::Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801955h
更新日期:2009-04-28 00:00:00
abstract::The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036338y
更新日期:2004-03-30 00:00:00
abstract::The C2A domain of synaptotagmin 7 (Syt7) is a Ca(2+) and membrane binding module that docks and inserts into cellular membranes in response to elevated intracellular Ca(2+) concentrations. Like other C2 domains, Syt7 C2A binds Ca(2+) and membranes primarily through three loop regions; however, it docks at Ca(2+) conce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00422
更新日期:2015-09-22 00:00:00
abstract::Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9014049
更新日期:2009-12-08 00:00:00
abstract::Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0498151
更新日期:2004-07-13 00:00:00
abstract::Purification and potential tachykinin and enkephalin precursor cleaving enzymes from bovine chromaffin granules was undertaken using as substrates the model precursors 35S-(Met)-beta-preprotachykinin [35S-(Met)-beta-PPT] and 35S-(Met)-preproenkephalin [35S-(Met)-PPE]. Purification by concanavalin A-Sepharose, Sephacry...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00132a011
更新日期:1992-05-05 00:00:00
abstract::The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00275a018
更新日期:1983-03-15 00:00:00
abstract::To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970932o
更新日期:1997-11-18 00:00:00
abstract::The aggregation of the microtubule-associated protein, tau, into amyloid fibrils is a hallmark of neurodegenerative diseases such as the tauopathies and Alzheimer's disease. Since monomeric tau is an intrinsically disordered protein and the polymeric fibrils possess an ordered cross-β core, the aggregation process is ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500528x
更新日期:2014-10-21 00:00:00
abstract::To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051669r
更新日期:2006-01-31 00:00:00
abstract::The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00045a002
更新日期:1995-11-14 00:00:00
abstract::It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00167a038
更新日期:1994-01-11 00:00:00
abstract::Vesicular stomatitis virus buds from domains of the plasma membrane that have a unique protein and lipid composition. Fluorescence digital imaging microscopy and resonance energy transfer were used to determine how the two viral envelope-associated proteins, the G and the M proteins, could alter the lateral distributi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a008
更新日期:1995-08-08 00:00:00
abstract::The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a003
更新日期:1983-05-10 00:00:00
abstract::Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061837v
更新日期:2006-12-26 00:00:00
abstract::Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00226a012
更新日期:1991-03-26 00:00:00
abstract::The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970177e
更新日期:1997-08-19 00:00:00
abstract::The PEGylated hemoglobin (Hb) has been evaluated as a potential blood substitute. In an attempt to understand the autoxidation of the PEGylated Hb, we have studied the autoxidation of the PEGylated Hb site-specifically modified at Cys-93(beta) or at Val-1(beta). PEGylation of Hb at Cys-93(beta) perturbed the heme envi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800906z
更新日期:2008-10-14 00:00:00
abstract::Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00408a045
更新日期:1988-04-19 00:00:00
abstract::The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901184r
更新日期:2009-09-29 00:00:00
abstract::Fluorescein isothiocyanate derivatization of the human lactotransferrin on Lys-264 inhibits the binding of the protein of human PHA-activated lymphocytes [Legrand, D., Mazurier, J., Maes, P., Rochard, E., Montreuil, J., & Spik, G. (1991) Biochem. J. 276, 733-738], indicating that part of the receptor-binding site is l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00153a018
更新日期:1992-09-29 00:00:00