Binding of ATP by pertussis toxin and isolated toxin subunits.

abstract：:Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...

journal_title：Biochemistry

authors： Merkle D,Block WD,Yu Y,Lees-Miller SP,Cramb DT

更新日期：2006-04-04 00:00:00

abstract：:The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation. In this reaction, the product, fXa, is transiently and reversibly bound to the TF-VIIa enzyme complex. This in effect leads to ...

journal_title：Biochemistry

authors： Hathcock J,Rusinova E,Vaananen H,Nemerson Y

更新日期：2007-05-22 00:00:00

abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...

journal_title：Biochemistry

authors： Engel J

更新日期：1992-11-10 00:00:00

abstract：:The flavocytochrome p-cresol methylhydroxylase from Pseudomonas putida has been reported to have a Mr of 114,000 and to consist of two subunits, a flavoprotein and a cytochrome c, each with a Mr of 58,000. Recent X-ray crystallographic data from our laboratories [Shamala, N., Lim, L. W., Mathews, F. S., McIntire, W., ...

journal_title：Biochemistry

authors： McIntire W,Singer TP,Smith AJ,Mathews FS

更新日期：1986-10-07 00:00:00

abstract：:Two short amino acid motifs, WXGDXNXR and PXWCDRXL, define a large family of inositol polyphosphate 5-phosphatases. We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphata...

journal_title：Biochemistry

authors： Jefferson AB,Majerus PW

更新日期：1996-06-18 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

journal_title：Biochemistry

authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

更新日期：1995-07-04 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:The enzyme phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the reversible conversion of oxalacetate and GTP to phosphoenolpyruvate (PEP), GDP, and CO2. PEPCK from higher organisms is a monomer, specifically requires GTP or ITP, and uses Mn2+ as the activating cation. Currently, there is no crystal structure of GTP...

journal_title：Biochemistry

authors： Holyoak T,Nowak T

更新日期：2001-09-18 00:00:00

abstract：:Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols and halides. The reaction mechanism involves the formation of a covalent alkyl-enzyme complex which is hydrolyzed by water. The active site is a hydrophobic cavity buried between the main domain and the cap domain of the enzyme. The enzyme ha...

journal_title：Biochemistry

authors： Schanstra JP,Janssen DB

更新日期：1996-05-07 00:00:00

abstract：:Cryospectrokinetic studies of zinc and cobalt carboxypeptidase A disclosed two intermediates in the hydrolysis of both peptides and depsipeptides and furnished all the rate and equilibrium constants for the reaction scheme E + S in equilibrium ES1 in equilibrium ES2---E + P [Auld, D. S., Galdes, A., Geoghegan, K. F., ...

journal_title：Biochemistry

authors： Galdes A,Auld DS,Vallee BL

更新日期：1986-02-11 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...

journal_title：Biochemistry

authors： Kuras R,Guergova-Kuras M,Crofts AR

更新日期：1998-11-17 00:00:00

abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...

journal_title：Biochemistry

authors： Mathews II,Vanderhoff-Hanaver P,Castellino FJ,Tulinsky A

更新日期：1996-02-27 00:00:00

abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...

journal_title：Biochemistry

authors： Gartner CA,Wen B,Wan J,Becker RS,Jones G 2nd,Gygi SP,Nelson SD

更新日期：2005-02-15 00:00:00

abstract：:We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

journal_title：Biochemistry

authors： Sineva EV,Davydov DR

更新日期：2010-12-21 00:00:00

abstract：:Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...

journal_title：Biochemistry

authors： Wang S,Guo ZY,Shen L,Zhang YJ,Feng YM

更新日期：2003-08-19 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-03-22 00:00:00

abstract：:The PEGylated hemoglobin (Hb) has been evaluated as a potential blood substitute. In an attempt to understand the autoxidation of the PEGylated Hb, we have studied the autoxidation of the PEGylated Hb site-specifically modified at Cys-93(beta) or at Val-1(beta). PEGylation of Hb at Cys-93(beta) perturbed the heme envi...

journal_title：Biochemistry

authors： Hu T,Li D,Manjula BN,Acharya SA

更新日期：2008-10-14 00:00:00

abstract：:Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...

journal_title：Biochemistry

authors： Ray TK,Das PK,Nandi J,Banerjee A,Bandopadhyay S

更新日期：1988-12-13 00:00:00

abstract：:The high-frequency resonance Raman spectra of FeIII yeast native cytochrome c peroxidase (CCP) and five of its mutants [CCP(MI), Phe-51, Leu-48, Lys-48, Asn-235, and Phe-191] were recorded in phosphate buffer, pH 7.0, and in glycerol/phosphate mixtures at 295 and 10 K. Glycerol induces heme coordination changes in som...

journal_title：Biochemistry

authors： Smulevich G,Mantini AR,English AM,Mauro JM

更新日期：1989-06-13 00:00:00

abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

journal_title：Biochemistry

authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

更新日期：1993-12-14 00:00:00

abstract：:The RelE and RelB proteins constitute the RNA interferase (toxin) and its cognate inhibitor (antitoxin) components of the Escherichia coli relBE toxin-antitoxin system. Despite the well-described functionality and physiological activity of this system in E. coli, no structural study was performed on the folding and st...

journal_title：Biochemistry

authors： Cherny I,Overgaard M,Borch J,Bram Y,Gerdes K,Gazit E

更新日期：2007-10-30 00:00:00

abstract：:In Campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-alpha-d-glucopyranose, termed N,N'-diacetylbacillosamine (Bac2,4diNAc), is the first carbohydrate in the glycoprotein N-linked heptasaccharide. With uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) as a starting point, two enzymes of the general protein glycos...

journal_title：Biochemistry

authors： Olivier NB,Chen MM,Behr JR,Imperiali B

更新日期：2006-11-14 00:00:00

abstract：:The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

journal_title：Biochemistry

authors： Dix TA,Bollag GE,Domanico PL,Benkovic SJ

更新日期：1985-06-04 00:00:00

abstract：:Inexpensive, straightforward, and rapid medical diagnostics are becoming increasingly important for disease identification in time- and resource-limited settings. Previous attempts to link oligonucleotide-based aptamers and hammerhead ribozymes to form ligand-induced ribozymes have been successful in identifying a var...

journal_title：Biochemistry

authors： Liao AM,Pan W,Benson JC,Wong AD,Rose BJ,Caltagirone GT

更新日期：2018-08-28 00:00:00

abstract：:Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...

journal_title：Biochemistry

authors： Nishimura S,Sasaki J,Kandori H,Matsuda T,Fukada Y,Maeda A

更新日期：1996-10-15 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...

journal_title：Biochemistry

authors： Sailer M,Helms GL,Henkel T,Niemczura WP,Stiles ME,Vederas JC

更新日期：1993-01-12 00:00:00