Abstract：
:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200 kDa), which together form the characteristic cross-shaped laminin structure with three short arms and one long arm. Many different domains have been identified in laminin by sequence analysis, structural investigations, and functional studies. Each short arm is formed by homologous N-terminal portions of one of the three chains. Structurally, each short arm contains two or three globular domains which are connected by rows of manyfold-repeated Cys-rich "EGF-like" domains. In all three chains this region is followed by a long heptad repeat region similar to those found in many alpha-helical coiled-coil proteins. These parts of the three laminin chains constitute a triple-stranded coiled-coil domain, which forms the extended rodlike structure of the long arm. This is the only domain in the protein which is made up of more than one chain and consequently serves the function of chain assembly. The two B chains are terminated by the coiled-coil domain, but the A chain contains an additional C-terminal segment which accounts for five globular domains located at the tip of the long arm. Several important functions of laminin have been assigned to individual domains in either the short arms or terminal regions of the long arm.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Engel Jdoi
10.1021/bi00159a001subject
Has Abstractpub_date
1992-11-10 00:00:00pages
10643-51issue
44eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract：:FixL is a heme-based oxygen-sensing histidine kinase that induces the expression of nitrogen fixation genes under hypoxic conditions. Oxygen dissociation from heme iron in the sensor domain of FixL initiates protein conformational changes that are transmitted to the histidine kinase domain, activating autophosphorylat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00405
更新日期：2016-07-26 00:00:00
abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971788+
更新日期：1997-12-09 00:00:00
abstract：:The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00140
更新日期：2015-04-21 00:00:00
abstract：:The homogeneous, single-walled phosphatidylcholine-cholesterol mixed vesicles were prepared by ultrasonic irradiation of egg phosphatidylcholine in the presence of various amounts of cholesterol in solution at 4 degrees under a nitrogen atmosphere followed by molecular sieve chromatography on a Sepharose 4B column. Ph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00686a012
更新日期：1975-07-29 00:00:00
abstract：:A ribonuclease that hydrolyzes either linear duplex or single-stranded RNA in an exonucleolytic manner has been partially purified from Ehrlich ascites tumor cell nucleoli and is free from other ribonucleases. The enzyme will also degrade the RNA complement of an RNA X DNA duplex; however, no nuclease activity is obse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00324a022
更新日期：1985-01-29 00:00:00
abstract：:Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00200a036
更新日期：1994-08-30 00:00:00
abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00644a004
更新日期：1977-12-13 00:00:00
abstract：:Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00450a028
更新日期：1989-11-28 00:00:00
abstract：:The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a028
更新日期：1986-05-20 00:00:00
abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201003a
更新日期：2011-11-01 00:00:00
abstract：:We have characterized the kinetics and substrate requirements of prenyl-flavin synthase from yeast. This enzyme catalyzes the addition of an isopentenyl unit to reduced flavin mononucleotide (FMN) to form an additional six-membered ring that bridges N5 and C6 of the flavin nucleus, thereby converting the flavin from a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01131
更新日期：2018-02-06 00:00:00
abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a016
更新日期：1993-08-10 00:00:00
abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00406a049
更新日期：1988-03-22 00:00:00
abstract：:The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034258o
更新日期：2004-01-20 00:00:00
abstract：:To determine whether ligand-receptor interactions could engender the selective uptake by deficient cells of enzyme-laden liposomes, aggregated human IgG was used to coat liposomes which had previously trapped purified hexosaminidase A (Hex A). By a new, high-yield procedure, Hex A was purified 7000-fold from human pla...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00647a034
更新日期：1976-01-27 00:00:00
abstract：:Several different monoclonal antibodies (mAbs) have been actively developed in the field of Alzheimer's disease (AD) for basic science and clinical applications; however, the binding kinetics of many of the mAbs with the beta-amyloid peptides (Abeta) are poorly understood. A panel of mAbs with different Abeta recognit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900523q
更新日期：2009-11-03 00:00:00
abstract：:Minibrain kinase/dual-specificity tyrosine phosphorylation regulated kinase 1A (MNB/DYRK1A) is a proline/arginine-directed serine/threonine kinase implicated in the learning deficits of Down syndrome. Epigallocatechin-3-gallate (EGCG), the major tea polyphenolic compound, is a potent MNB/DYRK1A inhibitor. In this stud...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060632j
更新日期：2006-10-03 00:00:00
abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00141a004
更新日期：1992-07-07 00:00:00
abstract：:Peptide inhibitors, specifically labeled at the alpha-amino terminus by dansylation, have been prepared by utilizing solid-phase peptide synthesis. Changes in fluorescence have been observed upon mixing these peptides with porcine pepsin that can be attributed to the formation of at least two complexes. Energy transfe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00528a023
更新日期：1981-12-08 00:00:00
abstract：:The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980795a
更新日期：1998-11-03 00:00:00
abstract：:Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900757q
更新日期：2009-09-29 00:00:00
abstract：:Deuterium-labeled dihydroorotates bearing one, two, or three deuteriums at the pair of C4 and C5 positions have been synthesized in high isotopic and chiral purity and characterized by NMR and mass spectroscopy. These substrates have been used with the FMN-containing biosynthetic dihydroorotate oxidase from Crithidia ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a033
更新日期：1984-06-05 00:00:00
abstract：:The respiratory chain of Vibrio cholerae contains three bd-type quinol oxygen reductases as well as one cbb(3) oxygen reductase. The cbb(3) oxygen reductase has been previously isolated and characterized; however, the natural mobile electron donor(s) that shuttles electrons between the bc(1) complex and the cbb(3) oxy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1004574
更新日期：2010-09-07 00:00:00
abstract：:Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and regulation of the tissue distribution of signaling molecules...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6025424
更新日期：2007-04-03 00:00:00
abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961099i
更新日期：1996-12-10 00:00:00
abstract：:Calpain-catalyzed proteolysis of II-spectrin is a regulated event associated with neuronal long-term potentiation, platelet and leukocyte activation, and other processes. Calpain proteolysis is also linked to apoptotic and nonapoptotic cell death following excessive glutamate exposure, hypoxia, HIV-gp120/160 exposure,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061504y
更新日期：2007-01-16 00:00:00
abstract：:Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9525177
更新日期：1996-06-25 00:00:00
abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701533j
更新日期：2008-01-22 00:00:00
abstract：:The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a003
更新日期：1982-05-11 00:00:00
abstract：:Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B. (1982) J. Biol. Chem. 257, 7608-7614; El Yandouzi, E. H., & Le Grimellec, C. (1992) Biochem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00174a046
更新日期：1994-03-01 00:00:00
