Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation.

abstract：:The addition of a small peptide can significantly increase the rate at which pepsin cleaves a substrate at pH 4.5. Why? In order to find out, we have determined spectrophotometrically the relative ability of over a dozen peptides to speed the initial rate of disappearance of Phe-Trp-NH2 and Leu-Trp-Met-Arg. Here are s...

journal_title：Biochemistry

authors： Silver MS,James SL

更新日期：1981-05-26 00:00:00

abstract：:The sialic acid binding lectin, limulin, was isolated by gel filtration and ion-exchange chromatography from the hemolymph of Limulus polyphemus. When the purified protein was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol, two major protein bands wer...

journal_title：Biochemistry

authors： Kaplan R,Li SS,Kehoe JM

更新日期：1977-09-20 00:00:00

abstract：:We describe the synthesis of bordifluoropyrromethene (BODIPY), fluorescein, and related fluorescent derivatives of the beta-adrenergic ligand CGP 12177. With these probes we screened insect (Sf9) cells stably transformed with the human beta 2-adrenoceptor gene and expressing (2-3.5) x 10(5) human beta 2-adrenoceptors ...

journal_title：Biochemistry

authors： Heithier H,Hallmann D,Boege F,Reiländer H,Dees C,Jaeggi KA,Arndt-Jovin D,Jovin TM,Helmreich EJ

更新日期：1994-08-09 00:00:00

abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

journal_title：Biochemistry

authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

更新日期：2007-05-22 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:Affinity selection-mass spectrometry (AS-MS) screening of kinesin spindle protein (KSP) followed by enzyme inhibition studies and temperature-dependent circular dichroism (TdCD) characterization was utilized to identify a series of benzimidazole compounds. This series also binds in the presence of Ispinesib, a known a...

journal_title：Biochemistry

authors： Sheth PR,Shipps GW Jr,Seghezzi W,Smith CK,Chuang CC,Sanden D,Basso AD,Vilenchik L,Gray K,Annis DA,Nickbarg E,Ma Y,Lahue B,Herbst R,Le HV

更新日期：2010-09-28 00:00:00

abstract：:The synthesis is described of four new cleavable, photosensitive, heterobifunctional cross-linking reagents for use in examining membrane organization: 4'-azidoazobenzene-4oxysuccinimide ester (1), N-[4-(p-azidophenylazo)benzoyl]-3-aminopropyl-N'-oxysuccinimide ester (2), N-[4-(p-azidophenylazo)benzoyl]-6-aminohexyl-N...

journal_title：Biochemistry

authors： Jaffe CL,Lis H,Sharon N

更新日期：1980-09-16 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:The interaction of the zinc finger protein WT1 with RNA aptamers has been investigated using a quantitative binding assay, and the results have been compared to those from a previous study of the DNA binding properties of this protein. A recombinant peptide containing the four zinc fingers of WT1 (WT1-ZFP) binds to re...

journal_title：Biochemistry

authors： Zhai G,Iskandar M,Barilla K,Romaniuk PJ

更新日期：2001-02-20 00:00:00

abstract：:Depletion of Ca2+ and/or Cl- ions from PSII membranes blocks the electron-transfer reactions that precede O2 evolution on the oxidizing side of the enzyme. Illumination of these inhibited preparations at 273 K generates a paramagnetic species that is detectable by low-temperature (T < 20 K) EPR as a signal in the g = ...

journal_title：Biochemistry

authors： Lydakis-Simantiris N,Dorlet P,Ghanotakis DF,Babcock GT

更新日期：1998-05-05 00:00:00

abstract：:Galectin-3 is a member of a large family of beta-galactoside-binding animal lectins and is composed of a carboxyl-terminal lectin domain connected to an amino-terminal nonlectin part. Previous experimental results suggest that, when bound to multivalent glycoconjugates, galectin-3 self-associates through intermolecula...

journal_title：Biochemistry

authors： Yang RY,Hill PN,Hsu DK,Liu FT

更新日期：1998-03-24 00:00:00

abstract：:The interaction of glutamyl-tRNA synthetase with tRNAGlu2 has been studied. The enzyme was purified to apparent homogeneity, and consists of a single chain with a molecular weight of 59 000. The sedimentation coefficient (sdegrees20,w) was found to be 3.7 S and suggests this enzyme is quite asymmetric. The enzyme bind...

journal_title：Biochemistry

authors： Willick GE,Kay CM

更新日期：1976-09-21 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

journal_title：Biochemistry

authors： Navarro J,Toivio-Kinnucan M,Racker E

更新日期：1984-01-03 00:00:00

abstract：:We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...

journal_title：Biochemistry

authors： Kuras R,Guergova-Kuras M,Crofts AR

更新日期：1998-11-17 00:00:00

abstract：:Mouse epidermal growth factor (mEGF), a protein hormone effector molecule that regulates cellular development and division, has been investigated by using proton nuclear magnetic resonance techniques at 500 MHz. Well-resolved downfield aromatic and alpha-CH proton resonances (5.0-8.0 ppm) and an upfield ring current s...

journal_title：Biochemistry

authors： Mayo KH

更新日期：1984-08-14 00:00:00

abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...

journal_title：Biochemistry

authors： Chi EY,Frey SL,Lee KY

更新日期：2007-02-20 00:00:00

abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...

journal_title：Biochemistry

authors： Piersma SR,Visser AJ,de Vries S,Duine JA

更新日期：1998-03-03 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:Human lecithin:cholesterol acyltransferase (LCAT, E.C.2.3.1.43) is a serine-type esterase that contains six cysteines, two of which, Cys31 and Cys184, are free. The remaining cysteines form disulfide links. One of these is between Cys50 and Cys74 and the other is between Cys313 and Cys356. The cDNA of LCAT and mutants...

journal_title：Biochemistry

authors： Qu SJ,Fan HZ,Blanco-Vaca F,Pownall HJ

更新日期：1993-03-30 00:00:00

abstract：:Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...

journal_title：Biochemistry

authors： Hayase T,Rice KG,Dziegielewska KM,Kuhlenschmidt M,Reilly T,Lee YC

更新日期：1992-05-26 00:00:00

abstract：:Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...

journal_title：Biochemistry

authors： Nishitani Y,Maruyama Y,Itoh T,Mikami B,Hashimoto W,Murata K

更新日期：2012-05-01 00:00:00

abstract：:The phosphorelay signal transduction pathway controls sporulation initiation in Bacillus subtilis. Transfer of a phosphoryl group from multiple kinases (KinA and KinB) through a single domain response regulator homologue (Spo0F), a phosphotransferase (Spo0B), and ultimately to a transcriptional regulator, (Spo0A) acti...

journal_title：Biochemistry

authors： Tzeng YL,Feher VA,Cavanagh J,Perego M,Hoch JA

更新日期：1998-11-24 00:00:00

abstract：:Over 155 mutations within the V2 vasopressin receptor (AVPR2) gene are responsible for nephrogenic diabetes insipidus (NDI). The expression and subcellular distribution of four of these was investigated in transfected cells. These include a point mutation in the seventh transmembrane domain (S315R), a frameshift mutat...

journal_title：Biochemistry

authors： Morello JP,Salahpour A,Petäjä-Repo UE,Laperrière A,Lonergan M,Arthus MF,Nabi IR,Bichet DG,Bouvier M

更新日期：2001-06-12 00:00:00

abstract：:Structure and dynamism in a DNA segment d-GATCTTCCCCCCGGAA have been investigated by nuclear magnetic resonance (NMR) spectroscopy. At neutral pH, the molecule exists largely as a dumbbell, formed by the association of two hairpins with sticky ends. A small percentage of hairpin is also detectable. The stem of the dum...

journal_title：Biochemistry

authors： Singh S,Patel PK,Hosur RV

更新日期：1997-10-28 00:00:00

abstract：:Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare t...

journal_title：Biochemistry

authors： McKenna CE,Simeonov AM,Eran H,Bravo-Leerabhandh M

更新日期：1996-04-09 00:00:00

abstract：:The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...

journal_title：Biochemistry

authors： Petithory JR,Jencks WP

更新日期：1988-11-15 00:00:00

abstract：:Electron transfer in reaction center core (RCC) complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum was studied by measuring flash-induced absorbance changes. The first preparation contained approximately three iron-sulfur centers, indicating that the three putative electron acce...

journal_title：Biochemistry

authors： Schmidt KA,Neerken S,Permentier HP,Hager-Braun C,Amesz J

更新日期：2000-06-20 00:00:00

abstract：:Pyridoxal 5'-triphospho-5'-adenosine (AP3-PL), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [Tagaya, M., & Fukui, T. (1986) Biochemistry 25, 2958-2964; Yagami, T., Tagaya, M., & Fukui, T. (1988) FEBS Lett. 229, 261-264], was used to identify the ATP-bindi...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Fukui T,Tagaya M,Blanquet S

更新日期：1990-12-25 00:00:00

abstract：:Tau is a neuronal microtubule-associated protein that plays a central role in many cellular processes, both physiological and pathological, such as axons stabilization and Alzheimer's disease. Despite extensive studies, very little is known about the detailed molecular basis of tau binding to microtubules. We used the...

journal_title：Biochemistry

authors： Devred F,Barbier P,Douillard S,Monasterio O,Andreu JM,Peyrot V

更新日期：2004-08-17 00:00:00

abstract：:A nine-base oligodeoxyribonucleotide complementary to bases 2497-2505 of 23S rRNA was hybridized to both 50S subunits and 70S ribosomes. The binding of the probe to the ribosome or ribosomal subunits was assayed by nitrocellulose filtration and by sucrose gradient centrifugation techniques. The location of the hybridi...

journal_title：Biochemistry

authors： Marconi RT,Hill WE

更新日期：1989-01-24 00:00:00