Abstract:
:Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myocardium, which was reversible upon insulin treatment. Analysis of acylcarnitine molecular species in myocardium unexpectedly identified acylcarnitine molecular species containing a mass shift of 16 amu in comparison to the anticipated molecular species. Synthesis of 3-hydroxy acylcarnitine identified the natural products as the 3-hydroxylated acylcarnitines through comparisons of diagnostic fragmentation patterns of synthetic and naturally occurring constituents using tandem mass spectrometry. Diabetes induced an increase of both calcium-independent phospholipase A(2) (iPLA(2)) mRNA and iPLA(2) activity in rat myocardium. Cardiac ischemia in myocardium genetically engineered to overexpress iPLA(2) dramatically increased the amount of acylcarnitine present in myocardium. Moreover, mechanism-based inactivation of iPLA(2) in either wild-type or transgenic myocardium ablated a substantial portion of the acylcarnitine increase. Collectively, these results identify discrete insulin remediable abnormalities in mitochondrial fatty acid processing in diabetic myocardium and identify iPLA(2) as an important enzymatic contributor to the pool of fatty acids that can be used for acylcarnitine synthesis and energy production in myocardium.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Su X,Han X,Mancuso DJ,Abendschein DR,Gross RWdoi
10.1021/bi047773asubject
Has Abstractpub_date
2005-04-05 00:00:00pages
5234-45issue
13eissn
0006-2960issn
1520-4995journal_volume
44pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Deuterium-labeled dihydroorotates bearing one, two, or three deuteriums at the pair of C4 and C5 positions have been synthesized in high isotopic and chiral purity and characterized by NMR and mass spectroscopy. These substrates have been used with the FMN-containing biosynthetic dihydroorotate oxidase from Crithidia ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00307a033
更新日期:1984-06-05 00:00:00
abstract::A ribonuclease was isolated from serum-free supernatants of the human colon adenocarcinoma cell line HT-29. It was purified by cation-exchange and C18 reversed-phase high-performance liquid chromatography. The protein is basic, has a molecular weight of approximately 16,000, and has an amino acid composition that is s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a002
更新日期:1986-11-18 00:00:00
abstract::In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501160q
更新日期:2015-01-20 00:00:00
abstract::The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961920q
更新日期:1997-02-11 00:00:00
abstract::The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00093a022
更新日期:1993-10-26 00:00:00
abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300429p
更新日期:2012-06-12 00:00:00
abstract::Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00152a020
更新日期:1992-09-22 00:00:00
abstract::Plasma high-density lipoprotein and apolipoprotein AI (apoAI) levels are suppressed by tumor necrosis factor alpha. To determine the molecular mechanisms responsible for the effect of TNF alpha on the apoAI promoter activity, HepG2 cells were exposed to both genetic and pharmacological modulators of TNF alpha-mediated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0518040
更新日期:2006-02-21 00:00:00
abstract::The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00608a028
更新日期:1978-07-25 00:00:00
abstract::A mechanism by which the Klenow fragment of DNA polymerase I monitors the geometry of the base pairs may involve hydrogen bonds between the polymerase and the minor groove of the nascent base pair. The involvement of the 3-position of guanine in the template strand was examined by synthesizing oligodeoxynucleotides co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971253g
更新日期:1997-10-28 00:00:00
abstract::The binding of heparin to high molecular weight kininogen (H-kininogen) was analyzed by the effect of kininogen in decreasing the heparin-induced enhancement of the rate of inactivation of thrombin by antithrombin. The conditions were arranged so that the heparin-catalyzed antithrombin-thrombin reaction, monitored in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a039
更新日期:1989-02-07 00:00:00
abstract::N-(1-Pyrene)maleimide is nonfluorescent in aqueous solution but forms strongly fluorescent adducts with sulfhydryl groups of organic compounds or proteins. The conjugation reactions of N-(1-pyrene)maleimide are relatively fast and can be monitored by the increase in fluorescence intensity of the pyrene chromophore. In...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00658a025
更新日期:1976-06-29 00:00:00
abstract::The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201603f
更新日期:2012-01-10 00:00:00
abstract::The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01084
更新日期:2020-04-07 00:00:00
abstract::Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00714
更新日期:2016-11-29 00:00:00
abstract::Channelrhodopsins (ChRs) from green flagellate algae function as light-gated ion channels when expressed heterologously in mammalian cells. Considerable interest has focused on understanding the molecular mechanisms of ChRs to bioengineer their properties for specific optogenetic applications such as elucidating the f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501243y
更新日期:2015-01-20 00:00:00
abstract::Chemical biosensors, for which chemical detection triggers a fluorescent signal, have the potential to accelerate the screening of noncolorimetric chemicals produced by microbes, enabling the high-throughput engineering of enzymes and metabolic pathways. Here, we engineer a G-protein-coupled receptor (GPCR)-based sens...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00605
更新日期:2017-10-17 00:00:00
abstract::Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the steady-state kinetic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00709
更新日期:2015-09-01 00:00:00
abstract::Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015935m
更新日期:2002-03-26 00:00:00
abstract::We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951584y
更新日期:1996-03-12 00:00:00
abstract::The formation of a complex between p21(ras) and GAP accelerates the GTPase reaction of p21(ras) and terminates the signal for cell proliferation. The understanding of this rate acceleration is important for the elucidation of the role of Ras mutants in tumor formation. In principle there are two main options for the o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000640e
更新日期:2000-08-15 00:00:00
abstract::Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801708f
更新日期:2008-12-16 00:00:00
abstract::The L-type Ca(2+) channel Ca(v)1.2 forms macromolecular signaling complexes that comprise the β(2) adrenergic receptor, trimeric G(s) protein, adenylyl cyclase, and cAMP-dependent protein kinase (PKA) for efficient signaling in heart and brain. The protein phosphatases PP2A and PP2B are part of this complex. PP2A coun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101018c
更新日期:2010-12-07 00:00:00
abstract::Calmodulin and troponin C exhibit calcium-dependent binding of 1 mol/mol of dynorphin. The dissociation constants of the complexes, determined in 0.20 N KC1-1.0 mM CaCI2, pH 7.3, are 0.6 microM for calmodulin, 2.4 microM for rabbit fast skeletal muscle troponin C, and 9 microM for bovine heart troponin C. Experiments ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00306a016
更新日期:1984-05-22 00:00:00
abstract::31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00507a013
更新日期:1981-02-17 00:00:00
abstract::We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00083a022
更新日期:1993-08-17 00:00:00
abstract::In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00300a002
更新日期:1984-02-28 00:00:00
abstract::This paper provides further characterization of a receptor that, in cells lacking the LDL receptor (FH fibroblasts), mediates lipoprotein binding, uptake, and degradation when incubated with oleate at concentrations not exceeding albumin binding capacity. This oleate-activated receptor is genetically distinct from the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00171a017
更新日期:1994-02-08 00:00:00
abstract::Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301350s
更新日期:2013-01-15 00:00:00
abstract::The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00386a011
更新日期:1987-06-16 00:00:00