Abstract：
:The primary structure of the J chain from a human Waldenströms IgM protein has been determined using a combination of automated and conventional Edman degradative procedures. Eighty-five percent of the sequence was established with peptides isolated from tryptic digests of carboxyamidomethylated and citraconylated J chain, many of which were sequenced completely. Alignment of the tryptic fragments was achieved with peptides generated by chymotrypsin and limited acid hydrolyses. The j chain consits of 129 amino acids and a single oligosaccharide structure linked to asparagine at positon 43 of the sequence. The molecular weight, including 7.5% carbohydrate by weight, is 16 422. The location and arrangement of three half-cystines could be deduced from previous studies, whereas the pairing of the remaining five disulfide bonds still needs to be clarified.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mole JE,Bhown AS,Bennett JCdoi
10.1021/bi00635a002subject
Has Abstractpub_date
1977-08-09 00:00:00pages
3507-13issue
16eissn
0006-2960issn
1520-4995journal_volume
16pub_type
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