Metal ion catalysis of RNA cleavage by the influenza virus endonuclease.

abstract：:Thyroxine and analogues inhibit rat liver aminoacyl-tRNA synthetase activity for phenylalanine and tyrosine. A high yield purification of the major cytoplasmic form of phenylalanyl-tRNA synthetase (C1) and its characterization is reported. Polyribosome-bound and other sedimentable forms are found to be indistinguishab...

journal_title：Biochemistry

authors： Nielsen JB,Haschemeyer AE

更新日期：1976-01-27 00:00:00

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:Preferential interaction of trans-membrane alpha-helices whose hydrophobic length matches the hydrophobic thickness of the lipid bilayer could be a mechanism of retention in the Golgi apparatus. We have used fluorescence methods to study the interaction of peptides Ac-K2-G-Lm-W-Ln-K2-A-amide (Pm+n) with bilayers of ph...

journal_title：Biochemistry

authors： Webb RJ,East JM,Sharma RP,Lee AG

更新日期：1998-01-13 00:00:00

abstract：:Ultraviolet absorption (UV) and circular dichroism (CD) spectra of wheat germ 5S RNA, when compared to tRNAPhe, indicate a largely base-paired and base-stacked helical structure, containing up to 36 base pairs. Fourier-transform infrared (FT-IR) spectra of tRNAPhe and wheat germ ribosomal 5S RNA have been acquired at ...

journal_title：Biochemistry

authors： Li SJ,Burkey KO,Luoma GA,Alben JO,Marshall AG

更新日期：1984-07-31 00:00:00

abstract：:The transcriptional activity of the beta-globin genes is regulated by a complex genetic element, the locus control region (LCR), at the 5'-end of the beta-globin locus. Tandem binding sites for the erythroid-specific transcription factor NF-E2 are important for the transcriptional activation function of the LCR. We di...

journal_title：Biochemistry

authors： Lam LT,Bresnick EH

更新日期：1995-12-19 00:00:00

abstract：:Stopped-flow kinetic measurements of the association of filipin with sterols in intact cells and isolated membranes of Mycoplasma capricolum were used to study the effects of varying the phospholipid in the membrane. The phospholipid composition and content of the membrane were varied by growing cells in an albumin-co...

journal_title：Biochemistry

authors： Clejan S,Bittman R,Rottem S

更新日期：1981-04-14 00:00:00

abstract：:A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...

journal_title：Biochemistry

authors： Nihira T,Mizuno M,Tonozuka T,Sakano Y,Mori T,Okahata Y

更新日期：2005-07-12 00:00:00

abstract：:The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：1988-10-04 00:00:00

abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...

journal_title：Biochemistry

authors： Johnson RM,Heslop CL,Deber CM

更新日期：2004-11-16 00:00:00

abstract：:A visual pigment consists of an opsin protein and a chromophore, 11-cis-retinal, which binds to a specific lysine residue of opsin via a Schiff base linkage. The Schiff base chromophore is protonated in pigments that absorb visible light, whereas it is unprotonated in ultraviolet-absorbing visual pigments (UV pigments...

journal_title：Biochemistry

authors： Tsutsui K,Imai H,Shichida Y

更新日期：2007-05-29 00:00:00

abstract：:The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...

journal_title：Biochemistry

authors： Winston SE,Mehan R,Falke JJ

更新日期：2005-09-27 00:00:00

abstract：:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...

journal_title：Biochemistry

authors： Seela F,Tran TQ,Mentzel H,Erdmann VA

更新日期：1981-04-28 00:00:00

abstract：:ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...

journal_title：Biochemistry

authors： Cupp-Vickery JR,Igarashi RY,Perez M,Poland M,Meyer CR

更新日期：2008-04-15 00:00:00

abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...

journal_title：Biochemistry

authors： Turchi JJ,Patrick SM,Henkels KM

更新日期：1997-06-17 00:00:00

abstract：:The structure of the His-94-->Cys variant of human carbonic anhydrase II (CAII) has been determined by X-ray crystallographic methods to a resolution of 2.3 A with a final crystallographic R factor of 0.155. This variant of CAII crystallizes in orthorhombic space group P2(1)2(1)2(1) which is the first example of a new...

journal_title：Biochemistry

authors： Alexander RS,Kiefer LL,Fierke CA,Christianson DW

更新日期：1993-02-16 00:00:00

abstract：:We have used resonance energy transfer to study the spatial relationship between Cys-98 of rabbit skeletal troponin C and Cys-133 of rabbit skeletal troponin I in the reconstituted ternary troponin complex. The donor was introduced by labeling either troponin C or troponin I with N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)...

journal_title：Biochemistry

authors： Tao T,Gowell E,Strasburg GM,Gergely J,Leavis PC

更新日期：1989-07-11 00:00:00

abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

journal_title：Biochemistry

authors： Koga K,Berliner LJ

更新日期：1985-12-03 00:00:00

abstract：:To study the substrate specificity and mechanism of thioltransferase (TTase) catalysis, we have used 14C- and 35S-radiolabeled mixed disulfides of cysteine and glutathione (GSH) with various cysteine-containing proteins. These protein mixed disulfide substrates were incubated with glutathione, glutathione disulfide (G...

journal_title：Biochemistry

authors： Gravina SA,Mieyal JJ

更新日期：1993-04-06 00:00:00

abstract：:DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

journal_title：Biochemistry

authors： Jorns MS,Wang B,Jordan SP

更新日期：1987-10-20 00:00:00

abstract：:Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...

journal_title：Biochemistry

authors： Hoffmann R,Lee VM,Leight S,Varga I,Otvos L Jr

更新日期：1997-07-01 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...

journal_title：Biochemistry

authors： Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

更新日期：2013-11-12 00:00:00

abstract：:The interaction of a divalent metal ion with a leaving 3' oxygen is a central component of several proposed mechanisms of phosphoryl transfer. In support of this are recent kinetic studies showing that thiophilic metal ions (e.g., Mn2+) stimulate the hydrolysis of compounds in which sulfur takes the place of the leavi...

journal_title：Biochemistry

authors： Brautigam CA,Sun S,Piccirilli JA,Steitz TA

更新日期：1999-01-12 00:00:00

abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

journal_title：Biochemistry

authors： Myers AG,Kort ME,Cohen SB,Tom NJ

更新日期：1997-04-01 00:00:00

abstract：:The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...

journal_title：Biochemistry

authors： Qin J,Clore GM,Gronenborn AM

更新日期：1996-01-09 00:00:00

abstract：:Biochemical properties of a detergent-solubilized tetrodotoxin binding component from Electrophorus electricus have been examined and compared with those found for the membrane-bound protein. The toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency ...

journal_title：Biochemistry

authors： Moore AC,Agnew WS,Raftery MA

更新日期：1982-11-23 00:00:00

abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...

journal_title：Biochemistry

authors： Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini A

更新日期：1994-05-31 00:00:00

abstract：:Stopped-flow fluorescence experiments have been carried out to study the steady-state kinetics of hydrolysis of Leu-Gly-NHNH-Dns [Dns = 5-(dimethylamino)naphthalene-1-sulfonyl] by porcine kidney cytosol leucine aminopeptidase (LAP) in 50% v/v methanol/buffer solution at ambient temperature and the pre-steady-state kin...

journal_title：Biochemistry

authors： Lin WY,Lin SH,Morris RJ,Van Wart HE

更新日期：1988-07-12 00:00:00

abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...

journal_title：Biochemistry

authors： Taylor EL,O'Brien PJ

更新日期：2015-01-27 00:00:00

abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...

journal_title：Biochemistry

authors： Powers R,Garrett DS,March CJ,Frieden EA,Gronenborn AM,Clore GM

更新日期：1992-05-05 00:00:00