Abstract:
:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate carboxylase. Both pyruvate kinase and phosphoenolpyruvate carboxylase convert the compound to thiopyruvate, which is a substrate for lactate dehydrogenase. Phosphoenolpyruvate carboxylase is slowly inactivated by phosphoenolthiopyruvate.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sikkema KD,O'Leary MHdoi
10.1021/bi00404a038subject
Has Abstractpub_date
1988-02-23 00:00:00pages
1342-7issue
4eissn
0006-2960issn
1520-4995journal_volume
27pub_type
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