Hemes a and a3 environments of plant cytochrome c oxidase.


:The structures of hemes a and a3 of maize and wheat germ cytochrome c oxidase were investigated by resonance Raman spectroscopy. Comparison between the plant and mammalian cytochrome oxidases revealed that (i) the vinyl groups associated with hemes a and a3 vibrate at higher frequencies in the plant enzyme than in the mammalian enzyme, suggesting different degrees of interaction between the heme cores and their periphery; (ii) aside from the geometry of the vinyl group, the structure of heme a3 in plant cytochrome oxidase is essentially unchanged from that of its mammalian counterpart; (iii) the vibrational band associated with the formyl group of reduced heme a shows relatively weak enhancement in the Soret-excited resonance Raman spectra of maize and wheat germ cytochrome oxidase, suggesting that the formyl group of ferrous heme a in the plant enzymes is conjugated only slightly to the porphyrin ring; and (iv) for oxidized heme a, the formyl vibration is strongly enhanced, but its frequency indicates a weaker interaction with the protein milieu relative to the mammalian enzyme. These observations suggest that the local environment around the formyl position of the heme a chromophore differs in the plant and mammalian cytochrome oxidases. The implication of the latter feature in the mechanism of proton pumping by cytochrome oxidase is discussed.






de Paula JC,Peiffer WE,Ingle RT,Centeno JA,Ferguson-Miller S,Babcock GT




Has Abstract


1990-09-18 00:00:00












  • Novel insights into the INK4-CDK4/6-Rb pathway: counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb.

    abstract::The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...


    pub_type: 杂志文章


    authors: Li J,Tsai MD

    更新日期:2002-03-26 00:00:00

  • Reconstitution of bovine procarboxypeptidase A-S6 from the free subunits.

    abstract::The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...


    pub_type: 杂志文章


    authors: Puigserver A,Desnuelle P

    更新日期:1977-05-31 00:00:00

  • The C2B domain of synaptotagmin I is a Ca2+-binding module.

    abstract::Synaptotagmin I is a synaptic vesicle protein that contains two C(2) domains and acts as a Ca(2+) sensor in neurotransmitter release. The Ca(2+)-binding properties of the synaptotagmin I C(2)A domain have been well characterized, but those of the C(2)B domain are unclear. The C(2)B domain was previously found to pull ...


    pub_type: 杂志文章


    authors: Ubach J,Lao Y,Fernandez I,Arac D,Südhof TC,Rizo J

    更新日期:2001-05-22 00:00:00

  • Mechanism of Agrobacterium beta-glucosidase: kinetic studies.

    abstract::The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...


    pub_type: 杂志文章


    authors: Kempton JB,Withers SG

    更新日期:1992-10-20 00:00:00

  • Thermodynamic and structural properties of the acid molten globule state of horse cytochrome C.

    abstract::To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...


    pub_type: 杂志文章


    authors: Nakamura S,Seki Y,Katoh E,Kidokoro S

    更新日期:2011-04-19 00:00:00

  • NSC 622124 inhibits human Eg5 and other kinesins via interaction with the conserved microtubule-binding site.

    abstract::Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...


    pub_type: 杂志文章


    authors: Learman SS,Kim CD,Stevens NS,Kim S,Wojcik EJ,Walker RA

    更新日期:2009-03-03 00:00:00

  • Role of alpha-synuclein carboxy-terminus on fibril formation in vitro.

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    pub_type: 杂志文章


    authors: Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

    更新日期:2003-07-22 00:00:00

  • Escherichia coli DNA-directed beta-galactosidase synthesis in presence and absence of Ca2+.

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    pub_type: 杂志文章


    authors: Jacobs KA,Schlessinger D

    更新日期:1977-03-08 00:00:00

  • Kinetic and mechanistic studies of the NO*-mediated oxidation of oxymyoglobin and oxyhemoglobin.

    abstract::The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...


    pub_type: 杂志文章


    authors: Herold S,Exner M,Nauser T

    更新日期:2001-03-20 00:00:00

  • Two distinct mechanisms for regulation of nonmuscle myosin assembly via the heavy chain: phosphorylation for MIIB and mts 1 binding for MIIA.

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    pub_type: 杂志文章


    authors: Murakami N,Kotula L,Hwang YW

    更新日期:2000-09-19 00:00:00

  • A Novel Soluble Peptide with pH-Responsive Membrane Insertion.

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    pub_type: 杂志文章


    authors: Nguyen VP,Alves DS,Scott HL,Davis FL,Barrera FN

    更新日期:2015-11-03 00:00:00

  • Crystal structure of human fibrinogen.

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    pub_type: 杂志文章


    authors: Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

    更新日期:2009-05-12 00:00:00

  • Serine92 (F7) contributes to the control of heme reactivity and stability in myoglobin.

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    pub_type: 杂志文章


    authors: Smerdon SJ,Krzywda S,Wilkinson AJ,Brantley RE Jr,Carver TE,Hargrove MS,Olson JS

    更新日期:1993-05-18 00:00:00

  • Communications between the high-affinity cyclic nucleotide binding sites in E. coli cyclic AMP receptor protein: effect of single site mutations.

    abstract::The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...


    pub_type: 杂志文章


    authors: Lin SH,Lee JC

    更新日期:2002-10-01 00:00:00

  • Sites of covalent attachment of CYP4 enzymes to heme: evidence for microheterogeneity of P450 heme orientation.

    abstract::Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...


    pub_type: 杂志文章


    authors: Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

    更新日期:2005-10-25 00:00:00

  • Mechanistic insight into the photosensory versatility of DXCF cyanobacteriochromes.

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    pub_type: 杂志文章


    authors: Rockwell NC,Martin SS,Lagarias JC

    更新日期:2012-05-01 00:00:00

  • Ligand-dependent aggregation and cooperativity: a critique.

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    pub_type: 杂志文章,评审


    authors: Saroff HA

    更新日期:1991-10-22 00:00:00

  • Rate-limiting step: a quantitative definition. Application to steady-state enzymic reactions.

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    pub_type: 杂志文章


    authors: Ray WJ Jr

    更新日期:1983-09-27 00:00:00

  • Molecular dynamics simulations of beta-ketoacyl-, beta-hydroxyacyl-, and trans-2-enoyl-acyl carrier proteins of Escherichia coli.

    abstract::Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...


    pub_type: 杂志文章


    authors: Chan DI,Tieleman DP,Vogel HJ

    更新日期:2010-04-06 00:00:00

  • Tandem GA residues on opposite sides of the loop in molecular beacon-like DNA hairpins compact the loop and increase hairpin stability.

    abstract::The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...


    pub_type: 杂志文章


    authors: Chang CY,Stellwagen NC

    更新日期:2011-10-25 00:00:00

  • Controlled reduction of the humidity induces a shortcut recovery reaction in the photocycle of photoactive yellow protein.

    abstract::The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We ...


    pub_type: 杂志文章


    authors: van der Horst MA,van Stokkum IH,Dencher NA,Hellingwerf KJ

    更新日期:2005-06-28 00:00:00

  • Multiple binding with identical linkage: a mechanism that explains the effect of lipoprotein(a) on fibrinolysis.

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    pub_type: 杂志文章


    authors: Hervio L,Durlach V,Girard-Globa A,Anglés-Cano E

    更新日期:1995-10-17 00:00:00

  • Phosphorylation modulates keratin structure.

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    pub_type: 杂志文章


    authors: Yeagle PL,Frye J,Eckert BS

    更新日期:1990-02-13 00:00:00

  • Unfolding and refolding of a type kappa immunoglobulin light chain and its variable and constant fragments.

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    pub_type: 杂志文章


    authors: Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

    更新日期:1987-09-22 00:00:00

  • Cdx1 interacts physically with a subset of Hox proteins.

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    pub_type: 杂志文章


    authors: Lafontaine CA,Grainger S,Hess BL,Béland M,Lohnes D

    更新日期:2012-12-04 00:00:00

  • Human secreted carbonic anhydrase: cDNA cloning, nucleotide sequence, and hybridization histochemistry.

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    pub_type: 杂志文章


    authors: Aldred P,Fu P,Barrett G,Penschow JD,Wright RD,Coghlan JP,Fernley RT

    更新日期:1991-01-15 00:00:00

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    pub_type: 杂志文章


    authors: Herrmann A,Clague MJ,Puri A,Morris SJ,Blumenthal R,Grimaldi S

    更新日期:1990-05-01 00:00:00

  • Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae.

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    pub_type: 杂志文章


    authors: Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

    更新日期:2006-10-31 00:00:00

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    pub_type: 杂志文章


    authors: Yamada M,Kuliopulos A,Nelson NP,Roth DA,Furie B,Furie BC,Walsh CT

    更新日期:1995-01-17 00:00:00

  • Sequential degradation of alphaII and betaII spectrin by calpain in glutamate or maitotoxin-stimulated cells.

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    pub_type: 杂志文章


    authors: Glantz SB,Cianci CD,Iyer R,Pradhan D,Wang KK,Morrow JS

    更新日期:2007-01-16 00:00:00