当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > The C-terminus is critical for the functional expression of the human serotonin transporter.

The C-terminus is critical for the functional expression of the human serotonin transporter.

Abstract：

:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficking to the plasma membrane. 5-HT uptake activity was used to measure the effects of systematic deletions or alanine substitutions in the C-terminus. We found that deletion of 16 amino acids in the distal C-terminus had no effect on uptake activity, whereas further deletion was detrimental for the function of SERT. Cell surface biotinylation was used to determine the role of the C-terminus in localization and trafficking. We showed that the C-terminus is crucial for the delivery of SERT to the plasma membrane and that the deletion of this part of the transporter results in a lack of mature glycosylation and impaired trafficking to the plasma membrane. Furthermore, the C-terminally truncated mutants were shown to have a dominant negative effect on wild-type SERT uptake activity.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Larsen MB,Fjorback AW,Wiborg O

doi

10.1021/bi0508688

subject

Has Abstract

pub_date

2006-01-31 00:00:00

pages

1331-7

issue

4

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Secondary structure and interaction of phage D108 Ner repressor with a 61-base-pair operator: evidence for altered protein and DNA structures in the complex.

    abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00201a018

    authors： Benevides JM,Kukolj G,Autexier C,Aubrey KL,DuBow MS,Thomas GJ Jr

    更新日期：1994-09-06 00:00:00

  • Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate.

    abstract：:Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00424a003

    authors： Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

    更新日期：1988-11-29 00:00:00

  • Structure of the stress response protein DR1199 from Deinococcus radiodurans: a member of the DJ-1 superfamily.

    abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800882v

    authors： Fioravanti E,Durá MA,Lascoux D,Micossi E,Franzetti B,McSweeney S

    更新日期：2008-11-04 00:00:00

  • Effects of pH on the S(3) state of the oxygen evolving complex in photosystem II probed by EPR split signal induction.

    abstract：:The electrons extracted from the CaMn(4) cluster during water oxidation in photosystem II are transferred to P(680)(+) via the redox-active tyrosine D1-Tyr161 (Y(Z)). Upon Y(Z) oxidation a proton moves in a hydrogen bond toward D1-His190 (His(Z)). The deprotonation and reprotonation mechanism of Y(Z)-OH/Y(Z)-O is of k...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101364t

    authors： Sjöholm J,Havelius KG,Mamedov F,Styring S

    更新日期：2010-11-16 00:00:00

  • Proton NMR studies of a metallothionein from Neurospora crassa: sequence-specific assignments by NOE measurements in the rotating frame.

    abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00433a038

    authors： Malikayil JA,Lerch K,Armitage IM

    更新日期：1989-04-04 00:00:00

  • The Ebola Viral Protein 35 N-Terminus Is a Parallel Tetramer.

    abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01154

    authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

    更新日期：2019-02-12 00:00:00

  • Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin.

    abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00364a007

    authors： Kristensen T,Saris CJ,Hunter T,Hicks LJ,Noonan DJ,Glenney JR Jr,Tack BF

    更新日期：1986-08-12 00:00:00

  • Structural locations and functional roles of new subsites S5, S6, and S7 in memapsin 2 (beta-secretase).

    abstract：:Memapsin 2 (beta-secretase) is the membrane-anchored aspartic protease that initiates the cleavage of beta-amyloid precursor protein (APP), leading to the production of amyloid-beta (Abeta), a major factor in the pathogenesis of Alzheimer's disease. The active site of memapsin 2 has been shown, with kinetic data and c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048106k

    authors： Turner RT 3rd,Hong L,Koelsch G,Ghosh AK,Tang J

    更新日期：2005-01-11 00:00:00

  • Stabilization of a protein nanocage through the plugging of a protein-protein interfacial water pocket.

    abstract：:The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200207w

    authors： Ardejani MS,Li NX,Orner BP

    更新日期：2011-05-17 00:00:00

  • Structure of an active soluble mutant of the membrane-associated (S)-mandelate dehydrogenase.

    abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010938k

    authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

    更新日期：2001-08-21 00:00:00

  • Direct identification of the agonist binding site in the human brain cholecystokininB receptor.

    abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990269z

    authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

    更新日期：1999-05-11 00:00:00

  • Interfering with the Dimerization of the ErbB Receptors by Transmembrane Domain-Derived Peptides Inhibits Tumorigenic Growth in Vitro and in Vivo.

    abstract：:The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00450

    authors： Bublil EM,Cohen T,Arnusch CJ,Peleg A,Pines G,Lavi S,Yarden Y,Shai Y

    更新日期：2016-10-04 00:00:00

  • Heterocycle formation in vibriobactin biosynthesis: alternative substrate utilization and identification of a condensed intermediate.

    abstract：:The iron-chelating peptide vibriobactin of the pathogenic Vibrio cholerae is assembled by a four-subunit nonribosomal peptide synthetase complex, VibE, VibB, VibH, and VibF, using 2,3-dihydroxybenzoate and L-threonine as precursors to two 2,3-dihydroxyphenyl- (DHP-) methyloxazolinyl groups in amide linkage on a norspe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010937s

    authors： Marshall CG,Burkart MD,Keating TA,Walsh CT

    更新日期：2001-09-04 00:00:00

  • Studies on the irreversible step of pepsinogen activation.

    abstract：:The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the condition...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00432a040

    authors： Glick DM,Shalitin Y,Hilt CR

    更新日期：1989-03-21 00:00:00

  • Characterization of a soluble adrenal phosphatidylinositol 4-kinase reveals wortmannin sensitivity of type III phosphatidylinositol kinases.

    abstract：:Phosphorylation of phosphatidylinositol (PtdIns) by PtdIns 4-kinases is the first step in the synthesis of polyphosphoinositides, the lipid precursors of intracellular signaling molecules. We have recently identified a cytosolic PtdIns 4-kinase (cPI4K) in the bovine adrenal cortex that is distinguished from previously...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9517493

    authors： Downing GJ,Kim S,Nakanishi S,Catt KJ,Balla T

    更新日期：1996-03-19 00:00:00

  • 4-Azido-2-nitrophenyl phosphate, a new photoaffinity derivative of inorganic phosphate. Study of its interaction with the inorganic phosphate binding site of beef heart mitochondrial adenosine triphosphatase.

    abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00561a013

    authors： Lauquin G,Pougeois R,Vignais PV

    更新日期：1980-09-30 00:00:00

  • Asymmetric Roles of Two Histidine Residues in Streptococcus pyogenes Cas9 Catalytic Domains upon Chemical Rescue.

    abstract：:CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00766

    authors： Furuhata Y,Kato Y

    更新日期：2021-01-26 00:00:00

  • Metal coordination sites that contribute to structure and catalysis in the group I intron from Tetrahymena.

    abstract：:We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00068a001

    authors： Christian EL,Yarus M

    更新日期：1993-05-04 00:00:00

  • Human transforming growth factor type beta 2: production by a prostatic adenocarcinoma cell line, purification, and initial characterization.

    abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00383a002

    authors： Ikeda T,Lioubin MN,Marquardt H

    更新日期：1987-05-05 00:00:00

  • Catalytic cycle of the phosphatidylcholine-preferring phospholipase C from Bacillus cereus. Solvent viscosity, deuterium isotope effects, and proton inventory studies.

    abstract：:The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc) is a 28.5 kDa enzyme with three zinc ions in its active site. Although much is known about the roles that various PLCBc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9821216

    authors： Martin SF,Hergenrother PJ

    更新日期：1999-04-06 00:00:00

  • Conformational flexibility of luteinizing hormone-releasing hormone in aqueous solution. A carbon-13 spin-lattice relaxation time study.

    abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00690a030

    authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

    更新日期：1975-09-23 00:00:00

  • Equilibrium analysis of ethidium binding to DNA containing base mismatches and branches.

    abstract：:In the processes of DNA replication, recombination, and repair, duplex DNA can transiently form branched structures, such as Holliday junctions, as well as base pair mismatches and bulges. These stages have altered ligand and protein binding properties from normal double helical DNA. A variety of ligands have been rep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00248a025

    authors： Hernández LI,Zhong M,Courtney SH,Marky LA,Kallenbach NR

    更新日期：1994-11-08 00:00:00

  • Metalation and Maturation of Zinc Ectoenzymes: A Perspective.

    abstract：:Numerous zinc ectoenzymes are folded and activated in the compartments of the early secretory pathway, such as the ER and the Golgi apparatus, before reaching their final destination. During this process, zinc must be incorporated into the active site; therefore, metalation of the nascent protein is indispensable for ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00924

    authors： Kambe T

    更新日期：2020-01-14 00:00:00

  • Iron regulatory element and internal loop/bulge structure for ferritin mRNA studied by cobalt(III) hexammine binding, molecular modeling, and NMR spectroscopy.

    abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9719814

    authors： Gdaniec Z,Sierzputowska-Gracz H,Theil EC

    更新日期：1998-02-10 00:00:00

  • Structural characteristics of heparan sulfates with varying sulfate contents.

    abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00629a014

    authors： Cifonelli JA,King JA

    更新日期：1977-05-17 00:00:00

  • Conformational properties of nine purified cystathionine β-synthase mutants.

    abstract：:Protein misfolding due to missense mutations is a common pathogenic mechanism in cystathionine β-synthase (CBS) deficiency. In our previous studies, we successfully expressed, purified, and characterized nine CBS mutant enzymes containing the following patient mutations: P49L, P78R, A114V, R125Q, E176K, R266K, P422L, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300435e

    authors： Hnízda A,Majtan T,Liu L,Pey AL,Carpenter JF,Kodíček M,Kožich V,Kraus JP

    更新日期：2012-06-12 00:00:00

  • Mechanistic insight into the photosensory versatility of DXCF cyanobacteriochromes.

    abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300171s

    authors： Rockwell NC,Martin SS,Lagarias JC

    更新日期：2012-05-01 00:00:00

  • The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex.

    abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00418a007

    authors： Darling TL,Fahnestock M

    更新日期：1988-09-06 00:00:00

  • Use of recombinant cell-permeable small peptides to modulate glucocorticoid sensitivity of acute lymphoblastic leukemia cells.

    abstract：:Glucocorticoid (GC) hormones induce apoptosis in T-cell and pre-B-cell acute lymphoblastic leukemia (ALL) cells. Steroid-mediated apoptosis requires a threshold level of the glucocorticoid receptor (GR) protein, and increasing the intracellular GR levels in ALL cells would augment their hormone sensitivity. A protein ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1007723

    authors： Geng CD,Vedeckis WV

    更新日期：2010-10-19 00:00:00

  • Heme binding properties of glyceraldehyde-3-phosphate dehydrogenase.

    abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300863a

    authors： Hannibal L,Collins D,Brassard J,Chakravarti R,Vempati R,Dorlet P,Santolini J,Dawson JH,Stuehr DJ

    更新日期：2012-10-30 00:00:00