Abstract：
:Escherichia coli were grown on 14.3% uniformly 13C-labeled glucose as the sole carbon source and challenged anaerobically with 90% 13C-labeled formaldehyde. The major multiply labeled metabolites were identified by 13C NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-propanediol. In each case, formaldehyde is incorporated only into the C1 position. A novel form of 13C NMR isotope dilution analysis of the major products reveals that all the 1,2-diol C1 is formaldehyde derived but that about 40% of the glycerol C1 is derived from bacterial sources. Glycerokinase converted the metabolite [1-13C]glycerol to equal amounts of [3-13C]glycerol 3-phosphate and [1-13C]glycerol 3-phosphate, demonstrating that the metabolite is racemic. When [13C]formaldehyde incubation was carried out in H2O/D2O mixtures, deuterium incorporation was detected by beta- and gamma-isotope shifts. The 1,3-diol is deuterium labeled only at C2 and only once, while the 1,2-diol and glycerol are each labeled independently at both C2 and C3; C3 is multiply labeled. Deuterium incorporation levels are different for each metabolite, indicating that the biosynthetic pathways probably diverge early.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hunter BK,Nicholls KM,Sanders JKdoi
10.1021/bi00336a050subject
Has Abstractpub_date
1985-07-16 00:00:00pages
4148-55issue
15eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a033
更新日期：1993-09-28 00:00:00
abstract：:In eukaryotic cells, structures called heterochromatin play critical roles in nuclear processes ranging from gene repression to chromosome segregation. Biochemical and in vivo studies over the past several decades have implied that the diverse functions of heterochromatin rely on the ability of these structures to spr...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.8b00401
更新日期：2018-05-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the histidine-containing phosphotransfer (HPt) protein YPD1 transfers phosphoryl groups between the three different response regulator domains of SLN1, SSK1, and SKN7 (designated R1, R2, and R3, respectively). Together these proteins form a branched histidine-aspartic acid phosphorelay sys...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048433s
更新日期：2005-01-11 00:00:00
abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0525223
更新日期：2006-03-21 00:00:00
abstract：:The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011001m
更新日期：2001-09-25 00:00:00
abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00178a009
更新日期：1994-03-29 00:00:00
abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a016
更新日期：1993-08-10 00:00:00
abstract：:The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00577a017
更新日期：1979-05-15 00:00:00
abstract：:A stable green heme was extracted from ferric cyanosulfmyoglobin after it had undergone an internal conversion reaction. After iron removal and conversion to the methyl ester, the resulting green porphyrin was purified by high-pressure liquid chromatography. Visible, 1H NMR, and mass spectrometric studies provided evi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00374a021
更新日期：1986-12-30 00:00:00
abstract：:The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides funnels excitation energy to the photosynthetic reaction center. Our ultimate goal is to build up the structure of LH1 from structures of its individual subunits, much as the antenna can self-assemble from its components in membrane-mimicking de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011576j
更新日期：2002-01-08 00:00:00
abstract：:The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9606253
更新日期：1996-09-24 00:00:00
abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00516a033
更新日期：1981-06-23 00:00:00
abstract：:A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00441a052
更新日期：1989-07-25 00:00:00
abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00573a028
更新日期：1979-03-20 00:00:00
abstract：:Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. Amino acid sequence alignments of the proteins from various sources suggested that several unusual di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00205a030
更新日期：1994-10-04 00:00:00
abstract：:Nitric oxide (NO) synthases (NOS) are thiolate-ligated heme-, tetrahydrobiopterin (BH(4))-, and flavin-containing monooxygenases which catalyze the NADPH-dependent conversion of L-arginine (L-Arg) to NO AND citrulline. NOS consists of two domains: an N-terminal oxygenase (heme- and BH(4)-bound) domain and a C-terminal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991580j
更新日期：1999-11-30 00:00:00
abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a022
更新日期：1992-05-26 00:00:00
abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00275a003
更新日期：1983-03-15 00:00:00
abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0480887
更新日期：2004-12-21 00:00:00
abstract：:To study the substrate specificity and mechanism of thioltransferase (TTase) catalysis, we have used 14C- and 35S-radiolabeled mixed disulfides of cysteine and glutathione (GSH) with various cysteine-containing proteins. These protein mixed disulfide substrates were incubated with glutathione, glutathione disulfide (G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a021
更新日期：1993-04-06 00:00:00
abstract：:The number of self-cleaving ribozymes has increased sharply in recent years, giving rise to elaborations of the four known ribozyme catalytic strategies, α, β, γ, and δ. One such extension is utilized by the twister ribozyme, which is hypothesized to conduct δ, or general acid catalysis, via N3 of the syn adenine +1 n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00742
更新日期：2019-12-03 00:00:00
abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00546a032
更新日期：1980-03-04 00:00:00
abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a013
更新日期：1976-09-21 00:00:00
abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00138a006
更新日期：1992-06-16 00:00:00
abstract：:We have employed high-performance liquid chromatography (HPLC) to investigate the syntheses of histones H1 and H1o as synchronized cells traverse from mitosis to S phase. Chinese hamster (line CHO) cells were synchronized by mitotic selection, and, at appropriate times, they were pulse labeled for 1 h with [3H]lysine....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a031
更新日期：1985-04-09 00:00:00
abstract：:The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00829
更新日期：2018-12-04 00:00:00
abstract：:The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc) is a 28.5 kDa enzyme with three zinc ions in its active site. Although much is known about the roles that various PLCBc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9821216
更新日期：1999-04-06 00:00:00
abstract：:Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00063a005
更新日期：1993-03-30 00:00:00
abstract：:The phosphorylation mechanism of Dictyostelium discoideum nucleoside diphosphate (NDP) kinase was investigated by NMR. 31P chemical shifts were measured on both native and denatured enzyme. In the enzymatically phosphorylated enzyme denatured by 9 M urea or 7 M guanidine hydrochloride, the NDP kinase phosphohistidine ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00038a043
更新日期：1995-09-26 00:00:00
abstract：:Reactivation of organophosphate (OP)-inhibited acetylcholinesterase (AChE) is a key objective in the treatment of OP poisoning. This study with native, wild-type, and mutant recombinant DNA-expressed AChEs, each inhibited by representative OP compounds, establishes a relationship between edrophonium acceleration of ox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9905720
更新日期：1999-08-03 00:00:00
