A Cu(I)-sensing ArsR family metal sensor protein with a relaxed metal selectivity profile.

abstract：:The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...

journal_title：Biochemistry

authors： Barbar E,Barany G,Woodward C

更新日期：1995-09-12 00:00:00

abstract：:Adriamycin plays a prominent role in the treatment of leukemia and solid tumors in man. The mode of interaction of adriamycin with its nuclear target, responsible for its therapeutic effect, is known [Berman, H. M., & Young, P.R. (1981) Annu. Rev. Biophys. Bioeng. 10, 87-114]. The planar anthracycline moiety of adriam...

journal_title：Biochemistry

authors： Goormaghtigh E,Brasseur R,Huart P,Ruysschaert JM

更新日期：1987-03-24 00:00:00

abstract：:All CG dinucleotides along exons 5-8 of the p53 tumor suppressor gene contain endogenous 5-methylcytosine (MeC). These same sites (e.g., codons 157, 158, 245, 248, and 273) are mutational hot spots in smoking-induced lung cancer. Several groups used the UvrABC endonuclease incision assay to demonstrate that methylated...

journal_title：Biochemistry

authors： Ziegel R,Shallop A,Upadhyaya P,Jones R,Tretyakova N

更新日期：2004-01-20 00:00:00

abstract：:We characterized endocytosis of iron-saturated (holo) and iron-depleted (apo) 125I-labeled bovine lactoferrin (Lf) by isolated rat hepatocytes. Hepatocytes ingested both Lf forms--determined by EGTA/dextran sulfate removal of surface-bound Lf--at maximal endocytic rates of 1.85 and 1.52 fmol cell-1 min-1 for 125I-apo-...

journal_title：Biochemistry

authors： McAbee DD,Nowatzke W,Oehler C,Sitaram M,Sbaschnig E,Opferman JT,Carr J,Esbensen K

更新日期：1993-12-14 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...

journal_title：Biochemistry

authors： Danyal K,Rasmussen AJ,Keable SM,Inglet BS,Shaw S,Zadvornyy OA,Duval S,Dean DR,Raugei S,Peters JW,Seefeldt LC

更新日期：2015-04-21 00:00:00

abstract：:The one-for-one exchange of Cl(-) and HCO(3)(-) ions is catalyzed by human erythrocyte anion exchanger 1 (hAE1) through a ping-pong mechanism whereby the protein exists in two main conformations, with the single anion-binding site exposed at either the cytoplasmic (inner) side (E(i)) or the extracellular side (E(o)), ...

journal_title：Biochemistry

authors： Pal P,Lebedev D,Salim S,Knauf PA

更新日期：2006-05-23 00:00:00

abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...

journal_title：Biochemistry

authors： Oyeyemi OA,Sours KM,Lee T,Kohen A,Resing KA,Ahn NG,Klinman JP

更新日期：2011-09-27 00:00:00

abstract：:Bicyclomycin is a novel, commercially important antibiotic. Information concerning the site of bicyclomycin inhibition in Escherichia coli has been obtained by the production of bicyclomycin resistant mutants by UV irradiation. Selection by growth in the presence of bicyclomycin of a plasmid clone library generated fr...

journal_title：Biochemistry

authors： Zwiefka A,Kohn H,Widger WR

更新日期：1993-04-13 00:00:00

abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...

journal_title：Biochemistry

authors： Hsieh PC,Kowalczyk TH,Phillips NF

更新日期：1996-07-30 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...

journal_title：Biochemistry

authors： Suh D,Povirk LF

更新日期：1997-04-08 00:00:00

abstract：:The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from bioche...

journal_title：Biochemistry

authors： Borer PN,Lin Y,Wang S,Roggenbuck MW,Gott JM,Uhlenbeck OC,Pelczer I

更新日期：1995-05-16 00:00:00

abstract：:Neutral solutes were used to investigate the effects of osmotic stress both on the ability of rhodopsin to undergo its activating conformation change and on acyl chain packing in the rod outer segment (ROS) disk membrane. The equilibrium concentration of metarhodopsin II (MII), the conformation of photoactivated rhodo...

journal_title：Biochemistry

authors： Mitchell DC,Litman BJ

更新日期：1999-06-15 00:00:00

abstract：:Hypoxia inducible factor-1 (HIF1) is a stress-responsive nuclear transcription factor that is activated with a decrease in oxygen availability. HIF1 regulates the expression of genes involved in a cell's adaptation to hypoxic stress, including those with mitochondrial specific function. To gain a more comprehensive un...

journal_title：Biochemistry

authors： Hwang HJ,Lynn SG,Vengellur A,Saini Y,Grier EA,Ferguson-Miller SM,LaPres JJ

更新日期：2015-06-23 00:00:00

abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

journal_title：Biochemistry

authors： Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

更新日期：1995-03-07 00:00:00

abstract：:The kinetics of formation of the ADP-sensitive (EP) and ADP-insensitive (E*P) phosphoenzyme intermediates of the CaATPase in sarcoplasmic reticulum (SR) were investigated by means of the quenched-flow technique. At 21 degrees C, addition of saturating ADP to SR vesicles phosphorylated for 116 ms with 10 microM ATP gav...

journal_title：Biochemistry

authors： Froehlich JP,Heller PF

更新日期：1985-01-01 00:00:00

abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

journal_title：Biochemistry

authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

更新日期：1987-03-24 00:00:00

abstract：:The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...

journal_title：Biochemistry

authors： Casadevall A,Day LA

更新日期：1988-05-17 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:(1) The rate of ATP synthesis during NADH-driven aerobic respiration has been measured in plasma membrane vesicles from Paracoccus denitrificans as a function of the concentration of the substrates, ADP and inorganic phosphate (Pi). In both cases, the response of the reaction to changes in the degree of saturation of ...

journal_title：Biochemistry

authors： Pérez JA,Ferguson SJ

更新日期：1990-11-20 00:00:00

abstract：:The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...

journal_title：Biochemistry

authors： Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

更新日期：1988-01-12 00:00:00

abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

journal_title：Biochemistry

authors： Kingsbury NL,Renegar RH,Chalovich JM

更新日期：2013-10-29 00:00:00

abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

journal_title：Biochemistry

authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

更新日期：2019-02-12 00:00:00

abstract：:TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...

journal_title：Biochemistry

authors： Fu H,Alvarez MA,Khosla C,Bailey JE

更新日期：1996-05-28 00:00:00

abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...

journal_title：Biochemistry

authors： Stoddard BL,Dean A,Koshland DE Jr

更新日期：1993-09-14 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

journal_title：Biochemistry

authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

更新日期：1995-09-12 00:00:00

abstract：:We have studied the effect of melittin, a basic membrane-binding peptide, on Ca-ATPase activity and on protein and lipid dynamics in skeletal sarcoplasmic reticulum (SR), using time-resolved phosphorescence and fluorescence spectroscopy. Melittin completely inhibits Ca-ATPase activity, with half-maximal inhibition at ...

journal_title：Biochemistry

authors： Voss J,Birmachu W,Hussey DM,Thomas DD

更新日期：1991-07-30 00:00:00

abstract：:Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...

journal_title：Biochemistry

authors： Fu I,Cai Y,Geacintov NE,Zhang Y,Broyde S

更新日期：2017-04-11 00:00:00

abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...

journal_title：Biochemistry

authors： Beechem JM,Sherman MA,Mas MT

更新日期：1995-10-24 00:00:00