Nucleotide cofactor-dependent structural change of Xenopus laevis Rad51 protein filament detected by small-angle neutron scattering measurements in solution.

abstract：:A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...

journal_title：Biochemistry

authors： Periasamy N,Armijo M,Verkman AS

更新日期：1991-12-24 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...

journal_title：Biochemistry

authors： Smitherman C,Rungsrisuriyachai K,Germann MW,Gadda G

更新日期：2015-01-20 00:00:00

abstract：:Ionizing radiation induces clustered DNA damage sites which have been shown to challenge the repair mechanism(s) of the cell. Evidence demonstrating that base excision repair is compromised during the repair of an abasic (AP) site present within a clustered damage site is presented. Simple bistranded clustered damage ...

journal_title：Biochemistry

authors： Lomax ME,Cunniffe S,O'Neill P

更新日期：2004-08-31 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:Alpha-sarcin is an exquisitely specific ribonuclease that binds and cleaves a single phosphodiester bond in the large rRNA of the eukaryotic ribosome, inactivating it. To better understand this remarkable activity, the contributions of the active site residues (His 50, Glu 96, and His 137) to the conformational stabil...

journal_title：Biochemistry

authors： García-Mayoral MF,del Pozo AM,Campos-Olivas R,Gavilanes JG,Santoro J,Rico M,Laurents DV,Bruix M

更新日期：2006-11-21 00:00:00

abstract：:Site-directed mutagenesis and random mutagenesis were used to produce variants of subtilisin BPN' (Bacillus amyloliquefaciens) protease with variable surface adsorption properties. Protease adsorption and peptide hydrolysis rate were measured for these variants using a model substrate consisting of a peptide covalentl...

journal_title：Biochemistry

authors： Brode PF 3rd,Erwin CR,Rauch DS,Barnett BL,Armpriester JM,Wang ES,Rubingh DN

更新日期：1996-03-12 00:00:00

abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...

journal_title：Biochemistry

authors： RoseFigura JM,Puehringer S,Schwarzenbacher R,Toyama H,Klinman JP

更新日期：2011-03-08 00:00:00

abstract：:The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...

journal_title：Biochemistry

authors： Delorme E,Lorenzini T,Giffin J,Martin F,Jacobsen F,Boone T,Elliott S

更新日期：1992-10-20 00:00:00

abstract：:Phase partition techniques have been used to measure the binding of the antitumor drugs adriamycin (NSC-123127) and daunorubicin (NSC-82151) to various DNAs. These methods provide reliable equilibrium binding data at the low levels of drug binding that may be expected in vivo. Both adriamycin and daunorubicin exhibit ...

journal_title：Biochemistry

authors： Graves DE,Krugh TR

更新日期：1983-08-02 00:00:00

abstract：:Translesion DNA synthesis represents the ability of a DNA polymerase to misinsert a nucleotide opposite a DNA lesion. Previous kinetic studies of the bacteriophage T4 DNA polymerase using a series of non-natural nucleotides suggest that pi-electron density of the incoming nucleotide substantially contributes to the ef...

journal_title：Biochemistry

authors： Zhang X,Donnelly A,Lee I,Berdis AJ

更新日期：2006-11-07 00:00:00

abstract：:A major regulatory step in the heat-induced transcription of heat shock protein (hsp) genes in eukaryotes is the activation of heat shock factor (HSF). In metazoans and Schizosaccharomyces pombe, HSF is present in unstressed cells but is unable to bind to its target DNA sequence element, the heat shock element (HSE). ...

journal_title：Biochemistry

authors： Larson JS,Schuetz TJ,Kingston RE

更新日期：1995-02-14 00:00:00

abstract：:L-alpha-Hydroxy acid oxidase (listed as EC 1.4.3.2, L-amino acid: O2 oxidoreductase) has been purified 100-fold from rat kidney to apparent homogeneity by gel electrophoresis. A subunit molecular weight of 47,500 was found by sodium dodecyl sulfate gel electrophoresis, but in contrast to previous reports, the enzyme h...

journal_title：Biochemistry

authors： Cromartie TH,Walsh CT

更新日期：1975-06-17 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:The ratio of the second-order rate constants for reduction of dihydroxyacetone phosphate (DHAP) and of the neutral truncated substrate glycolaldehyde (GLY) by glycerol 3-phosphate dehydrogenase (NAD (+), GPDH) saturated with NADH is (1.0 x 10 (6) M (-1) s (-1))/(8.7 x 10 (-3) M (-1) s (-1)) = 1.1 x 10 (8), which was u...

journal_title：Biochemistry

authors： Tsang WY,Amyes TL,Richard JP

更新日期：2008-04-22 00:00:00

abstract：:The functional interaction of the recombinant alpha 2 adrenergic receptor subtypes, alpha 2-C10 (the human platelet alpha 2 receptor, equivalent to the alpha 2 A subtype) and alpha 2-C4 (an alpha 2 receptor subtype cloned from a human kidney cDNA library), with G proteins was characterized in an in vitro reconstitutio...

journal_title：Biochemistry

authors： Kurose H,Regan JW,Caron MG,Lefkowitz RJ

更新日期：1991-04-02 00:00:00

abstract：:In this study, we have investigated the influence of regions outside the DNA-binding domain of the human glucocorticoid receptor on high-affinity DNA binding. We find that the DNA-binding domain shows a 10-fold lower affinity for a palindromic DNA-binding site than the intact receptor. The N-terminal part of the recep...

journal_title：Biochemistry

authors： Dahlman-Wright K,Wright AP,Gustafsson JA

更新日期：1992-09-22 00:00:00

abstract：:Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...

journal_title：Biochemistry

authors： Santoro MM,Liu Y,Khan SM,Hou LX,Bolen DW

更新日期：1992-06-16 00:00:00

abstract：:Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...

journal_title：Biochemistry

authors： Bull GD,Thompson KC

更新日期：2018-07-31 00:00:00

abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...

journal_title：Biochemistry

authors： AbdulSalam SF,Thowfeik FS,Merino EJ

更新日期：2016-09-27 00:00:00

abstract：:Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...

journal_title：Biochemistry

authors： O'Reilly M,Watson KA,Johnson LN

更新日期：1999-04-27 00:00:00

abstract：:The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...

journal_title：Biochemistry

authors： Bhat S,Sorci-Thomas MG,Tuladhar R,Samuel MP,Thomas MJ

更新日期：2007-07-03 00:00:00

abstract：:Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...

journal_title：Biochemistry

authors： Platzer G,Schedlbauer A,Chemelli A,Ozdowy P,Coudevylle N,Auer R,Kontaxis G,Hartl M,Miles AJ,Wallace BA,Glatter O,Bister K,Konrat R

更新日期：2011-07-12 00:00:00

abstract：:The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...

journal_title：Biochemistry

authors： Hofmann K,Zhang WJ,Romovacek H,Finn FM,Bothner-By AA,Mishra PK

更新日期：1984-06-05 00:00:00

abstract：:Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...

journal_title：Biochemistry

authors： Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

更新日期：2006-02-28 00:00:00

abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

journal_title：Biochemistry

authors： Nath A,Atkins WM

更新日期：2008-01-08 00:00:00

abstract：:The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar reflections on the meridian, and other reflections lie on a ser...

journal_title：Biochemistry

authors： Blaurock AE,McIntosh TJ

更新日期：1986-01-28 00:00:00

abstract：:Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...

journal_title：Biochemistry

authors： Kisiel W,Davie EW

更新日期：1975-11-04 00:00:00

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00

abstract：:The three-dimensional solution structure of the repeating -PO4-6Gal beta 1-4Man alpha 1- disaccharide fragment of the lipophosphoglycan (LPG) derived from Leishmania donovani has been determined by use of a combination of homo- and heteronuclear NMR spin coupling constant measurements together with restrained molecula...

journal_title：Biochemistry

authors： Homans SW,Mehlert A,Turco SJ

更新日期：1992-01-28 00:00:00