Abstract：
:In this study, we have investigated the influence of regions outside the DNA-binding domain of the human glucocorticoid receptor on high-affinity DNA binding. We find that the DNA-binding domain shows a 10-fold lower affinity for a palindromic DNA-binding site than the intact receptor. The N-terminal part of the receptor protein does not influence its DNA-binding affinity, while the C-terminal steroid-binding domain increases the DNA-binding affinity of the receptor molecule. It has previously been shown that both the intact glucocorticoid receptor and the glucocorticoid receptor DNA-binding domain bind to a palindromic glucocorticoid response element on DNA as dimers. It is likely that differences in DNA-binding affinity observed result from protein-protein interactions outside the DNA-binding domain between receptor monomers, as has been shown for the estrogen receptor. We have previously identified a segment involved in protein-protein interactions between DNA-binding domains of glucocorticoid receptors. This, in combination with results presented in this study, suggests that there are at least two sites of contact between receptor monomers bound to DNA. We suggest that the interaction between the DNA-binding domains may act primarily to restrict DNA binding to binding sites with appropriate half-site spacing and that additional stability of the receptor dimer is provided by the interactions between the steroid-binding domains.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dahlman-Wright K,Wright AP,Gustafsson JAdoi
10.1021/bi00152a047subject
Has Abstractpub_date
1992-09-22 00:00:00pages
9040-4issue
37eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800882v
更新日期：2008-11-04 00:00:00
abstract：:The fluorescence decay of apoazurin derived from Pseudomonas aeruginosa is monoexponential. By this criterion the population of molecules of apoazurin is homogeneous. The emission anisotropy factor and the absorption anisotropy factor at the red edge of the absorption band assume similar values, showing that the trypt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00680a018
更新日期：1975-05-06 00:00:00
abstract：:A GTP:RNA guanylyltransferase or capping enzyme has been purified approximately 2000-fold from wheat germ. The enzyme catalyzes the transfer of the GMP residue from GTP to the 5' end of RNA or synthetic polyribonucleotides. Diphosphate-ended polymers were capped more efficiently than molecules with triphosphate ends, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a020
更新日期：1982-01-19 00:00:00
abstract：:The vitamin D receptor (VDR) binds 1,25-dihydroxyvitamin D(3) and mediates its actions on gene transcription by heterodimerizing with retinoid X receptors (RXRs) on direct repeat (DR+3) vitamin D responsive elements (VDREs) located in target genes. The VDRE binding function of VDR has been primarily ascribed to the zi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9916574
更新日期：1999-12-07 00:00:00
abstract：:A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a004
更新日期：1995-01-24 00:00:00
abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0601003
更新日期：2006-05-23 00:00:00
abstract：:Photoaffinity labeling of the nicotinic acetylcholine receptor from Torpedo marmorata electric tissue was performed in the presence of cholinergic effectors in the millisecond to second time range by a combination of a stopped-flow apparatus and a high-energy pulse laser. The label applied was [3H]triphenylmethylphosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00307a029
更新日期：1984-06-05 00:00:00
abstract：:Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0607352
更新日期：2006-08-08 00:00:00
abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00156a015
更新日期：1992-10-20 00:00:00
abstract：:The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a003
更新日期：1982-05-11 00:00:00
abstract：:Alanine scanning mutagenesis, double mutant cycles, and X-ray crystallography were used to characterize the interface between the anti-hen egg white lysozyme (HEL) antibody D1.3 and HEL. Twelve out of the 13 nonglycine contact residues on HEL, as determined by the high-resolution crystal structure of the D1.3-HEL comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980148j
更新日期：1998-06-02 00:00:00
abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9528006
更新日期：1996-06-11 00:00:00
abstract：:Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a018
更新日期：1988-02-23 00:00:00
abstract：:The P-glycoprotein multidrug transporter functions as an ATP-driven efflux pump for a large number of structurally unrelated hydrophobic compounds. Substrates are believed to gain access to the transporter after partitioning into the membrane, rather than from the extracellular aqueous phase. The binding of drug subst...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990064q
更新日期：1999-05-25 00:00:00
abstract：:Activation of a growth factor receptor tyrosine kinase (RTK) is accompanied by a rapid autophosphorylation of the receptor on tyrosine residues. Receptor activation has been shown to promote the association of signal-transducing proteins containing SH2 domains (second domain of src homology). These receptor-associated...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a028
更新日期：1993-09-28 00:00:00
abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047422w
更新日期：2005-05-24 00:00:00
abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00390a019
更新日期：1987-08-11 00:00:00
abstract：:A novel phosphotriesterase was recently discovered and purified from Sphingobium sp. TCM1 (Sb-PTE) and shown to catalyze the hydrolysis of a broad spectrum of organophosphate esters with a catalytic efficiency that exceeds 10(6) M(-1) s(-1) for the hydrolysis of triphenyl phosphate. The enzyme was crystallized and the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00364
更新日期：2016-07-19 00:00:00
abstract：:In this paper, we examined the posttranslational modifications (PTMs) of high-mobility group A1 (HMGA1) proteins in PC-3 human prostate cancer cells that are either treated or not treated with a histone deacetylase inhibitor, sodium butyrate. We found that, from a reversed-phase C4 column, the HMGA1a protein eluted in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060504v
更新日期：2006-06-13 00:00:00
abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972247n
更新日期：1998-05-05 00:00:00
abstract：:Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00193
更新日期：2016-06-21 00:00:00
abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8021558
更新日期：2009-06-02 00:00:00
abstract：:The full-length cDNA encoding a novel human intracellular serine proteinase inhibitor has been sequenced and found to encode a 376 amino acid protein (M(r) approximately 42.5K) that we designate as cytoplasmic antiproteinase. Analysis of the primary structure revealed that the cytoplasmic antiproteinase has the majori...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00177a037
更新日期：1994-03-22 00:00:00
abstract：:The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular replacement, based on the structure of the B2 domain (Brookhaven accession code 1PGX). Final R values are 0.174 and 0.198 fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：
更新日期：1994-04-19 00:00:00
abstract：:The multicatalytic proteinase complex (MPC, proteasome) is composed of 28 subunits organized into four rings surrounding a water-filled canal. The catalytic centers face the inner canal confining protein substrates to an enclosed space. Experimental findings obtained with MPC from archaebacteria suggest that degradati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990826h
更新日期：1999-11-02 00:00:00
abstract：:In an effort to extend automated Edman degradation to nanomole quantities of protein, the method of sequenator analysis described by Edman and Begg (Edman, P., and Begg, G. (1967), Eur. J. Biochem. 1, 80) was modified to permit long degradations in the absence of carrier proteins. By using an aqueous 0.1 M Quadrol pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00684a036
更新日期：1975-07-01 00:00:00
abstract：:Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00691a008
更新日期：1975-10-07 00:00:00
abstract：:MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981066y
更新日期：1998-09-08 00:00:00
abstract：:Calmodulin and troponin C exhibit calcium-dependent binding of 1 mol/mol of dynorphin. The dissociation constants of the complexes, determined in 0.20 N KC1-1.0 mM CaCI2, pH 7.3, are 0.6 microM for calmodulin, 2.4 microM for rabbit fast skeletal muscle troponin C, and 9 microM for bovine heart troponin C. Experiments ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00306a016
更新日期：1984-05-22 00:00:00
abstract：:ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00243a005
更新日期：1991-07-23 00:00:00
