Acetylation and phosphorylation of high-mobility group A1 proteins in PC-3 human tumor cells.


:In this paper, we examined the posttranslational modifications (PTMs) of high-mobility group A1 (HMGA1) proteins in PC-3 human prostate cancer cells that are either treated or not treated with a histone deacetylase inhibitor, sodium butyrate. We found that, from a reversed-phase C4 column, the HMGA1a protein eluted in two different fractions with distinct forms of PTMs: Ser98, Ser101, and Ser102 were phosphorylated and Arg25 was methylated for both fractions; only the minor fraction, however, is hyperphosphorylated where Ser35, Thr52, and Thr77 were also phosphorylated. In addition, Lys14 was acetylated in the major but not the minor HMGA1a fraction isolated from the PC-3 cells that were not treated with butyrate. Likewise, HMGA1b, which is a splicing variant of HMGA1a, was acetylated on Lys14 and phosphorylated on the corresponding residues, i.e., Thr41, Thr66, Ser87, Ser90, and Ser91. The acetylation and phosphorylation of the HMGA1a and HMGA1b proteins may affect their interactions with other protein factors, which in turn may modulate the binding of HMGA1 proteins to DNA and regulate gene expression. In addition, the specifically posttranslationally modified HMGA1 proteins may serve as molecular biomarkers for cancer diagnosis and prognosis.






Jiang X,Wang Y




Has Abstract


2006-06-13 00:00:00












  • Chromatin structural changes in synchronized cells blocked in early S phase by sequential use of isoleucine deprivation and hydroxyurea blockade.

    abstract::We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...


    pub_type: 杂志文章


    authors: D'Anna JA,Prentice DA

    更新日期:1983-11-22 00:00:00

  • Mapping the interfacial binding surface of human secretory group IIa phospholipase A2.

    abstract::Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...


    pub_type: 杂志文章


    authors: Snitko Y,Koduri RS,Han SK,Othman R,Baker SF,Molini BJ,Wilton DC,Gelb MH,Cho W

    更新日期:1997-11-25 00:00:00

  • Disrupted zinc-binding sites in structures of pathogenic SOD1 variants D124V and H80R.

    abstract::Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...


    pub_type: 杂志文章


    authors: Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

    更新日期:2010-07-13 00:00:00

  • Structural and functional analysis of the HIV gp41 core containing an Ile573 to Thr substitution: implications for membrane fusion.

    abstract::The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...


    pub_type: 杂志文章


    authors: Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

    更新日期:2001-03-06 00:00:00

  • Microtubule dynamic instability does not result from stabilization of microtubules by tubulin-GDP-Pi subunits.

    abstract::The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...


    pub_type: 杂志文章


    authors: Caplow M,Shanks J

    更新日期:1998-09-15 00:00:00

  • Hydrolysis of phosphatidylcholine by hepatic lipase in discoidal and spheroidal recombinant high-density lipoprotein.

    abstract::Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...


    pub_type: 杂志文章


    authors: Tansey JT,Thuren TY,Jerome WG,Hantgan RR,Grant K,Waite M

    更新日期:1997-10-07 00:00:00

  • Structural investigation of the binding of nucleotide to phosphoenolpyruvate carboxykinase by NMR.

    abstract::The enzyme phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the reversible conversion of oxalacetate and GTP to phosphoenolpyruvate (PEP), GDP, and CO2. PEPCK from higher organisms is a monomer, specifically requires GTP or ITP, and uses Mn2+ as the activating cation. Currently, there is no crystal structure of GTP...


    pub_type: 杂志文章


    authors: Holyoak T,Nowak T

    更新日期:2001-09-18 00:00:00

  • Optimization of alternate-strand triple helix formation at the 5'CpG3' and 5'GpC3' junction steps.

    abstract::Oligonucleotide-directed triple helix formation normally requires a long tract of oligopyrimidine.oligopurine sequence. This limitation can be partially overcome by alternate-strand triple helix (or switch triple helix) formation which enables recognition of alternating oligopurine/oligopyrimidine sequences. The prese...


    pub_type: 杂志文章


    authors: Marchand C,Sun JS,Bailly C,Waring MJ,Garestier T,Hélène C

    更新日期:1998-09-22 00:00:00

  • Ethidium bromide complexes with self-complementary deoxytetranucleotides. Demonstration and discussion of sequence preferences in the intercalative binding of ethidium bromide.

    abstract::The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...


    pub_type: 杂志文章


    authors: Kastrup RV,Young MA,Krugh TR

    更新日期:1978-11-14 00:00:00

  • Formation of a porphyrin pi-cation radical in the fluoride complex of horseradish peroxidase.

    abstract::Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...


    pub_type: 杂志文章


    authors: Farhangrazi ZS,Sinclair R,Powers L,Yamazaki I

    更新日期:1995-11-21 00:00:00

  • Interaction between Cellobiose Dehydrogenase and Lytic Polysaccharide Monooxygenase.

    abstract::Lytic polysaccharide monooxygenases (LPMOs) are ubiquitous oxidoreductases, facilitating the degradation of polymeric carbohydrates in biomass. Cellobiose dehydrogenase (CDH) is a biologically relevant electron donor in this process, with the electrons resulting from cellobiose oxidation being shuttled from the CDH de...


    pub_type: 杂志文章


    authors: Laurent CVFP,Breslmayr E,Tunega D,Ludwig R,Oostenbrink C

    更新日期:2019-03-05 00:00:00

  • Chemical communication across the zinc tetrathiolate cluster in Escherichia coli Ada, a metalloactivated DNA repair protein.

    abstract::The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...


    pub_type: 杂志文章


    authors: Sun LJ,Yim CK,Verdine GL

    更新日期:2001-09-25 00:00:00

  • A vibrational analysis of the catalytically important C4-H bonds of NADH bound to lactate or malate dehydrogenase: ground-state effects.

    abstract::We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the f...


    pub_type: 杂志文章


    authors: Deng H,Zheng J,Sloan D,Burgner J,Callender R

    更新日期:1992-06-02 00:00:00

  • Cofactor Complexes of DesD, a Model Enzyme in the Virulence-related NIS Synthetase Family.

    abstract::The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...


    pub_type: 杂志文章


    authors: Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

    更新日期:2020-09-22 00:00:00

  • Histone-dependent reconstitution and nucleosomal localization of a nonhistone chromosomal protein: the H2A-specific protease.

    abstract::We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...


    pub_type: 杂志文章


    authors: Watson DK,Moudrianakis EN

    更新日期:1982-01-19 00:00:00

  • Site-specific incorporation of (aminooxy)acetic acid into proteins.

    abstract::By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein bio...


    pub_type: 杂志文章


    authors: Eisenhauer BM,Hecht SM

    更新日期:2002-09-24 00:00:00

  • Calorimetric examination of high-affinity Src SH2 domain-tyrosyl phosphopeptide binding: dissection of the phosphopeptide sequence specificity and coupling energetics.

    abstract::SH2 domains are protein modules which interact with specific tyrosine phosphorylated sequences in target proteins. The SH2 domain of the Src kinase binds with high affinity to a tyrosine phosphorylated peptide containing the amino acids Glu, Glu, and Ile (EEI) at the positions +1, +2, and +3 C-terminal to the phosphot...


    pub_type: 杂志文章


    authors: Bradshaw JM,Waksman G

    更新日期:1999-04-20 00:00:00

  • Mapping the Plasticity of the Escherichia coli Genetic Code with Orthogonal Pair-Directed Sense Codon Reassignment.

    abstract::The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...


    pub_type: 杂志文章


    authors: Schmitt MA,Biddle W,Fisk JD

    更新日期:2018-05-15 00:00:00

  • Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1.

    abstract::Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...


    pub_type: 杂志文章


    authors: Kim PW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

    更新日期:2014-05-06 00:00:00

  • Inhibition of CMP-sialic acid transport into Golgi vesicles by nucleoside monophosphates.

    abstract::We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...


    pub_type: 杂志文章


    authors: Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

    更新日期:2001-11-27 00:00:00

  • Conformational states of the bacteriophage P22 capsid subunit in relation to self-assembly.

    abstract::The formation of closed icosahedral capsids from a single species of coat protein subunit requires that the subunits assume different conformations at different lattice positions. In the double-stranded DNA bacteriophage P22, formation of correctly dimensioned capsids is mediated by interaction between coat protein su...


    pub_type: 杂志文章


    authors: Prevelige PE Jr,Thomas D,King J,Towse SA,Thomas GJ Jr

    更新日期:1990-06-12 00:00:00

  • Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin A receptor.

    abstract::The interaction of the C-terminal octapeptide of cholecystokinin, CCK-8, with the third extracellular loop of human cholecystokinin-A receptor, CCK(A)-R(329-357), has been probed by high-resolution NMR and extensive computer simulations. The structure of CCK(A)-R(329-357) in the presence of dodecylphosphocholine micel...


    pub_type: 杂志文章


    authors: Giragossian C,Mierke DF

    更新日期:2001-04-03 00:00:00

  • Novel spin-labels for the study of lipid-protein interactions. Application to (Na+, K+)-ATPase membranes.

    abstract::The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...


    pub_type: 杂志文章


    authors: Esmann M,Hideg K,Marsh D

    更新日期:1988-05-31 00:00:00

  • Resonance energy transfer as a direct monitor of GTP-binding protein-effector interactions: activated alpha-transducin binding to the cGMP phosphodiesterase in the bovine phototransduction cascade.

    abstract::Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...


    pub_type: 杂志文章


    authors: Erickson JW,Cerione RA

    更新日期:1991-07-23 00:00:00

  • Crystal and molecular structure of a new Z-DNA crystal form: d[CGT(2-NH2-A)CG] and its platinated derivative.

    abstract::The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essenti...


    pub_type: 杂志文章


    authors: Parkinson GN,Arvanitis GM,Lessinger L,Ginell SL,Jones R,Gaffney B,Berman HM

    更新日期:1995-11-28 00:00:00

  • Photolysis of "purple" lipoxygenase: implications for the structure of the chromophore.

    abstract::Treatment of soybean lipoxygenase isozyme 1 with its substrates, linoleic acid and oxygen, or product, 13(S)-hydroperoxy-9,11(Z,E)-octadecadienoic acid (13-HPOD), leads to the appearance of a purple color. Although the structure of the chromophore has not been determined, we present strong evidence that it is an Fe(3+...


    pub_type: 杂志文章


    authors: Nelson MJ,Chase DB,Seitz SP

    更新日期:1995-05-09 00:00:00

  • Conformational characteristics of deoxyribonucleic acid-butylamine complexes with C-type circular dichroism spectra. 2. A Raman spectroscopic study.

    abstract::The derivatives of calf thymus DNA in which n-butylamine is covalently attached as described in the preceding paper in this series [Chen, C. Y., Pheiffer, B. H., Zimmerman, S. B., & Hanlon, S. (1983) Biochemistry (preceding paper in this issue)] were examined by Raman spectroscopy. As previously mentioned, these compl...


    pub_type: 杂志文章


    authors: Fish SR,Chen CY,Thomas GJ Jr,Hanlon S

    更新日期:1983-09-27 00:00:00

  • A flavin-dependent sulfhydryl oxidase in bovine milk.

    abstract::Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disulfide bonds with the reduction of oxygen to hydrogen peroxide. The first mammalian sulfhydryl oxidase to be described was an iron-dependent enzyme isolated from bovine milk whey (Janolino, V.G., and Swaisgood, H.E. (1975) J. Biol. C...


    pub_type: 杂志文章


    authors: Jaje J,Wolcott HN,Fadugba O,Cripps D,Yang AJ,Mather IH,Thorpe C

    更新日期:2007-11-13 00:00:00

  • Spin-label electron paramagnetic resonance and differential scanning calorimetry studies of the interaction between mitochondrial cytochrome c oxidase and adenosine triphosphate synthase complex.

    abstract::The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...


    pub_type: 杂志文章


    authors: Qiu ZH,Yu L,Yu CA

    更新日期:1992-03-31 00:00:00

  • Characterization of the ubiquinone binding site in the alternative NADH-quinone oxidoreductase of Saccharomyces cerevisiae by photoaffinity labeling.

    abstract::The Ndi1 enzyme found in the mitochondrial membrane of Saccharomyces cerevisiae is an NDH-2-type alternative NADH-quinone oxidoreductase. As Ndi1 is expected to be a possible remedy for complex I defects of mammalian mitochondria, a detailed biochemical characterization of the enzyme is needed. To identify the ubiquin...


    pub_type: 杂志文章


    authors: Murai M,Yamashita T,Senoh M,Mashimo Y,Kataoka M,Kosaka H,Matsuno-Yagi A,Yagi T,Miyoshi H

    更新日期:2010-04-06 00:00:00