Abstract：
:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric forms by sedimentation velocity analysis and chemical cross-linking. G protein was modified with either fluorescein isothiocyanate or rhodamine isothiocyanate. Resonance energy transfer between fluorescein and rhodamine labels was observed upon mixing the two labeled G proteins in octyl glucoside. This result provided further evidence that G protein in octyl glucoside is oligomeric and indicated that the subunits are capable of exchange to form mixed oligomers. Resonance energy transfer was independent of G protein concentration in the range examined (10-80 nM) and was not observed when labeled G proteins were mixed with fluorescein or rhodamine that was not conjugated to protein. Resonance energy transfer decreased upon incorporation of G protein into Triton X-100, consistent with sedimentation velocity data that G protein in Triton X-100 is primarily monomeric. Kinetic analysis showed that the subunit exchange reaction had a half-time of about 3 min at 27 degrees C that was independent of G protein concentration. These data indicate that the exchange occurs through dissociation of G protein trimers into monomers and dimers followed by reassociation into timers. Thus, in octyl glucoside, G protein must exist as an equilibrium between monomers and oligomers. This implies that monomers are capable of self-assembly into trimers.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lyles DS,Varela VA,Parce JWdoi
10.1021/bi00462a002subject
Has Abstractpub_date
1990-03-13 00:00:00pages
2442-9issue
10eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962884y
更新日期：1997-04-29 00:00:00
abstract：:Thermal transitions of type I collagen fibrils were investigated by differential scanning calorimetry and spectrophotometry of turbidity within a wide range of external conditions. The advanced microcalorimeter allowed us to carry out the measurements at low concentrations of collagen (0.15-0.3 mg/mL). At these concen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980360n
更新日期：1998-06-02 00:00:00
abstract：:The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00538a007
更新日期：1982-04-27 00:00:00
abstract：:The NikR transcription factor from Escherichia coli is a Ni(II)-dependent repressor that regulates production of the nickel ion transporter encoded by the nik operon. In the previous paper in this issue (Wang, S. C., Dias, A., Bloom, S. L., and Zamble, D. B. (2004) Selectivity of Metal Binding and Metal-Induced Stabil...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049404k
更新日期：2004-08-10 00:00:00
abstract：:The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025872n
更新日期：2002-06-25 00:00:00
abstract：:Apolipoprotein E (apoE)-knockout mice develop severe atherosclerosis associated with high levels of very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) in plasma. To investigate the atherogenic role of VLDL and IDL, the lipoprotein fraction containing both VLDL and IDL (apoEko-VLDL/IDL) was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980762v
更新日期：1998-09-29 00:00:00
abstract：:Ultraviolet (uv)-light-mediated formation of protein-DNA adducts in Chinese hamster cell chromatin was investigated in an attempt to compare chromatin alterations induced in vitro with those observed in vivo. Three independent methods of analysis indicated stable protein-DNA associations: (1) a membrane filter assay w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00653a019
更新日期：1976-04-20 00:00:00
abstract：:Myo1c is an unconventional myosin involved in cell signaling and membrane dynamics. Calcium binding to the regulatory-domain-associated calmodulin affects myo1c motor properties, but the kinetic details of this regulation are not fully understood. We performed actin gliding assays, ATPase measurements, fluorescence sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700894h
更新日期：2007-10-23 00:00:00
abstract：:A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050079q
更新日期：2005-07-12 00:00:00
abstract：:Reduced intracellular drug accumulation due to the activity of the drug efflux pump ABC (B1) is a major mechanism in the resistance of cancer cells to chemotherapy. ABC (B1) is a poly specific transporter, and the molecular mechanism of its complex translocation process remains to be elucidated. To understand the proc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7023089
更新日期：2008-03-25 00:00:00
abstract：:Monte Carlo protein simulations with continuum solvation were used to explore the conformational mobility of NO within the active site of metmyoglobin. To the best of our knowledge this is the first application of a continuum solvation model for exploring protein binding sites. The usefulness of the Monte Carlo confor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981611v
更新日期：1999-05-18 00:00:00
abstract：:The solution conformation of the ribonuclease barnase has been determined by using 1H nuclear magnetic resonance (NMR) spectroscopy. The 20 structures were calculated by using 853 interproton distance restraints obtained from analyses of two-dimensional nuclear Overhauser spectra, 72 phi and 53 chi 1 torsion angle res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00099a030
更新日期：1991-09-03 00:00:00
abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9923611
更新日期：2000-04-11 00:00:00
abstract：:The 24-kDa subunit of mitochondrial NADH-ubiquinone reductase (complex I) is an iron-sulfur protein that is present in the flavoprotein or NADH dehydrogenase II subcomplex. It is a nuclear gene product and is imported into the organelle. A group of human patients with mitochondrial myopathy have been shown to have red...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00434a021
更新日期：1989-04-18 00:00:00
abstract：:X-ray crystal structures of enzyme-ligand complexes are widely believed to mimic states in the catalytic cycle, but this presumption has seldom been carefully scrutinized. In the case of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase (IMPDH), 10 structures of various enzyme-substrate-inhibitor complexes ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101590c
更新日期：2010-12-21 00:00:00
abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi953025x
更新日期：1996-05-21 00:00:00
abstract：:Geobacillus stearothermophilus T-6 encodes for a beta-xylosidase (XynB2) from family 52 of glycoside hydrolases that was previously shown to hydrolyze its substrate with net retention of the anomeric configuration. XynB2 significantly prefers substrates with xylose as the glycone moiety and exhibits a typical bell-sha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034505o
更新日期：2003-09-09 00:00:00
abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9904151
更新日期：1999-06-29 00:00:00
abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00642a600
更新日期：1977-11-15 00:00:00
abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00583a005
更新日期：1979-08-07 00:00:00
abstract：:Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantita...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00120a011
更新日期：1992-02-11 00:00:00
abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991888a
更新日期：1999-12-21 00:00:00
abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026778+
更新日期：2003-02-04 00:00:00
abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00020a014
更新日期：1995-05-23 00:00:00
abstract：:Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972562+
更新日期：1998-05-19 00:00:00
abstract：:Expression of the Tn21 mercury-resistance (mer) locus is controlled by the merR gene product, which represses mer structural gene (merTPCAD) transcription in the absence of mercuric ion [Hg(II)] and activates it in the presence of Hg(II). In vivo DNA methylation of the mer regulatory region (merOP) shows that, with or...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00493a011
更新日期：1990-10-16 00:00:00
abstract：:The aromatic residues of fd coat protein in sodium dodecyl sulfate micelles are characterized by 1H and 13C NMR. Resonances from both types of nuclei show structure-induced chemical shift dispersion and line widths indicative of a folded native structure for the protein. The two tyrosines were found to have pKas of 12...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00505a010
更新日期：1981-01-20 00:00:00
abstract：:Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801708f
更新日期：2008-12-16 00:00:00
abstract：:The successive high-resolution differential scanning calorimetric (DSC) thermograms for aqueous dispersions of a homologous series of mixed-chain phosphatidylcholines, C(X):C(X + 6)PC, have been recorded and analyzed. In this series of saturated mixed-chain phosphatidylcholines, the total number of carbon atoms in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00482a018
更新日期：1990-07-31 00:00:00
abstract：:During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00446
更新日期：2017-07-18 00:00:00
