Kinetic characterization of recombinant human acidic mammalian chitinase.

abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

journal_title：Biochemistry

authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

更新日期：2001-12-18 00:00:00

abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

journal_title：Biochemistry

authors： Lennon BW,Williams CH Jr

更新日期：1996-04-16 00:00:00

abstract：:Mg2+ is important for the RNase activity of the hammerhead ribozyme. To investigate the binding properties of Mg2+ to the hammerhead ribozyme, cleavage rates and CD spectra for substrates containing inosine or guanosine at the cleavage site were measured. The 2-amino group of this guanosine interfered with the rate of...

journal_title：Biochemistry

authors： Koizumi M,Ohtsuka E

更新日期：1991-05-28 00:00:00

abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

journal_title：Biochemistry

authors： Nath A,Atkins WM

更新日期：2008-01-08 00:00:00

abstract：:Complete resonance assignments of nonexchangeable base protons and sugar protons in d-GGTACGCGTACC at 500 MHz have been obtained by two-dimensional correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The characteristic phase-sensitive multiplet patterns of the ntrasugar cross peaks ...

journal_title：Biochemistry

authors： Chary KV,Hosur RV,Govil G,Chen CQ,Miles HT

更新日期：1988-05-17 00:00:00

abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

journal_title：Biochemistry

authors： Jin L,Yang J,Carey J

更新日期：1993-07-20 00:00:00

abstract：:Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...

journal_title：Biochemistry

authors： Mei R,Herschlag D

更新日期：1996-05-07 00:00:00

abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

journal_title：Biochemistry

authors： Dewey TG,Turner DH

更新日期：1980-04-15 00:00:00

abstract：:Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...

journal_title：Biochemistry

authors： Vandemeulebroucke A,Minici C,Bruno I,Muzzolini L,Tornaghi P,Parkin DW,Versées W,Steyaert J,Degano M

更新日期：2010-10-19 00:00:00

abstract：:The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...

journal_title：Biochemistry

authors： Rao BK,Tyryshkin AM,Roberts AG,Bowman MK,Kramer DM

更新日期：2000-03-28 00:00:00

abstract：:Exchangeable imidazole NH proton nuclear magnetic resonance (NMR) signals of the proximal histidine F8, which is directly coordinated to the paramagnetic porphyrin cobalt ion, have been measured for cobalt-substituted deoxyhemoglobins (deoxy-CoHb) and iron-cobalt hybrid hemoglobins, alpha (Co)2 beta (Fe)2 and alpha (F...

journal_title：Biochemistry

authors： Inubushi T,Ikeda-Saito M,Yonetani T

更新日期：1983-06-07 00:00:00

abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...

journal_title：Biochemistry

authors： Gestl EE,Eckert KA

更新日期：2005-05-10 00:00:00

abstract：:DNA (cytosine-5-)-methyltransferase is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methylation patterns. Steady-state and substrate trapping studies were performed to better understand how the enzyme functions. The catalytic efficiency was de...

journal_title：Biochemistry

authors： Flynn J,Reich N

更新日期：1998-10-27 00:00:00

abstract：:Peptidylglycine alpha-hydroxylating monooxygenase (PHM) is a copper, ascorbate, and molecular oxygen dependent enzyme that catalyzes the first step leading to the C-terminal amidation of glycine-extended peptides. The catalytic core of PHM (PHMcc), refined to residues 42-356 of the PHM protein, was expressed at high l...

journal_title：Biochemistry

authors： Kolhekar AS,Keutmann HT,Mains RE,Quon AS,Eipper BA

更新日期：1997-09-09 00:00:00

abstract：:DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...

journal_title：Biochemistry

authors： Jacobs KA,Schlessinger D

更新日期：1977-03-08 00:00:00

abstract：:The beta subunits of the 143-kDa alpha2beta2 tetrameric enzyme tryptophan synthase have been labeled by L-[ring-4-19F]phenylalanine and L-[phenol-4-13C]tyrosine in an effort to monitor the positions of these residues on ligand binding. Of the 13 phenylalanine and 11 tyrosine residues in the beta subunit, only three pa...

journal_title：Biochemistry

authors： McDowell LM,Lee M,McKay RA,Anderson KS,Schaefer J

更新日期：1996-03-12 00:00:00

abstract：:Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...

journal_title：Biochemistry

authors： Andreola ML,Tarrago-Litvak L,Levina AS,Kolocheva TI,el Dirani-Diab R,Jamkovoy VI,Khalimskaya NL,Barr PJ,Litvak S,Nevinsky GA

更新日期：1993-04-13 00:00:00

abstract：:Endogenous lectins induce effects on cell growth by binding to antennae of natural glycoconjugates. These complex carbohydrates often present more than one potential lectin-binding site in a single chain. Using the growth-regulatory interaction of the pentasaccharide of ganglioside GM(1) with homodimeric galectin-1 on...

journal_title：Biochemistry

authors： Siebert HC,André S,Lu SY,Frank M,Kaltner H,van Kuik JA,Korchagina EY,Bovin N,Tajkhorshid E,Kaptein R,Vliegenthart JF,von der Lieth CW,Jiménez-Barbero J,Kopitz J,Gabius HJ

更新日期：2003-12-23 00:00:00

abstract：:Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...

journal_title：Biochemistry

authors： Omoto CK,Johnson KA

更新日期：1986-01-28 00:00:00

abstract：:A photoaffinity probe for corticotropin (ACTH) receptors was prepared by selective modification of the single tryptophan residue in ACTH. A new photoreactive agent, 2,4-dinitro-5-azidophenylsulfenyl chloride, was synthesized and used for introducing the photoreactive group into ACTH. 2,4-Dinitro-5-azidophenylsulfenyl-...

journal_title：Biochemistry

authors： Canova-Davis E,Ramachandran J

更新日期：1980-07-08 00:00:00

abstract：:Incorporation of nonproteinogenic amino acids in small polypeptides synthesized by nonribosomal peptide synthetases (NRPS) significantly contributes to their biological activity. In these peptides, conversion of L-amino acids to the corresponding D-isomer is catalyzed by specialized NRPS modules that utilize an epimer...

journal_title：Biochemistry

authors： Linne U,Doekel S,Marahiel MA

更新日期：2001-12-25 00:00:00

abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

journal_title：Biochemistry

authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

更新日期：2008-01-22 00:00:00

abstract：:The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt c...

journal_title：Biochemistry

authors： Hirai M,Niimura N,Zama M,Mita K,Ichimura S,Tokunaga F,Ishikawa Y

更新日期：1988-10-04 00:00:00

abstract：:Kinetic analysis and modeling studies of HIV-1 and HIV-2 proteinases were carried out using the oligopeptide substrate [formula: see text] and its analogs containing single amino acid substitutions in P3-P3' positions. The two proteinases acted similarly on the substrates except those having certain hydrophobic amino ...

journal_title：Biochemistry

authors： Tözsér J,Weber IT,Gustchina A,Bláha I,Copeland TD,Louis JM,Oroszlan S

更新日期：1992-05-26 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semialdehyde by the addition of H(2)O to the C-2, C-3 double bond, followed by the loss of HCl from the C-3 position. Sequence similarity between CaaD, an (alphabeta)(3) heterohexamer (molecular weight 47,547), and 4-oxalocr...

journal_title：Biochemistry

authors： Azurmendi HF,Wang SC,Massiah MA,Poelarends GJ,Whitman CP,Mildvan AS

更新日期：2004-04-13 00:00:00

abstract：:DNA transaction reactions require formation of nucleoprotein complexes that involve multifaceted DNA-protein and protein-protein interactions. Genetic and biochemical studies suggested that the higher order Gal repressosome structure, which governs the transcription of two tandem galpromoters in Escherichia coli, invo...

journal_title：Biochemistry

authors： Roy S,Dimitriadis EK,Kar S,Geanacopoulos M,Lewis MS,Adhya S

更新日期：2005-04-12 00:00:00

abstract：:We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....

journal_title：Biochemistry

authors： Roche CJ,Thomson JA,Crothers DM

更新日期：1994-02-01 00:00:00

abstract：:The positions, with respect to the plasma membrane, of lysine 905, contained in the peptide QRKIVE, and of lysine 1012, contained in the carboxy-terminal peptide, RPGGWVEKETYY, of ovine Na+/K(+)-transporting ATPase have been reported to be cytoplasmic and extracytoplasmic, respectively [Bayer, R. (1990) Biochemistry 2...

journal_title：Biochemistry

authors： Thibault D

更新日期：1993-03-23 00:00:00