当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Interparticle interactions and structural changes of nucleosome core particles in low-salt solution.

Interparticle interactions and structural changes of nucleosome core particles in low-salt solution.

Abstract：

:The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt concentration, the appearance of a scattering peak that is assignable to interparticle interactions, an intraparticle structural transition, a decrease in the sedimentation velocity of the particle, and a release of bound Na+ ions from the particle are all observed concurrently when the ratio of solvent Na+ ions per particle is below approximately 1000. These observations are interpreted to indicate that a release of bound Na+ ions from the particle brings about structural rearrangements and weakens the electrostatic shielding of the particle, and this introduces long-range repulsive ordering of the particle in low-salt solution. Analyses of the scattering data indicate that the rearrangement within the core particle in low-salt solution is slight, changing the particle's shape slightly from cylindrical to a more spherical form by moving the center of the mass of the DNA somewhat inward with accompanying small decreases in the radii of gyration of both the DNA and the histones.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Hirai M,Niimura N,Zama M,Mita K,Ichimura S,Tokunaga F,Ishikawa Y

doi

10.1021/bi00420a051

subject

Has Abstract

pub_date

1988-10-04 00:00:00

pages

7924-31

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

27

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate.

    abstract：:Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00424a003

    authors： Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

    更新日期：1988-11-29 00:00:00

  • The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily.

    abstract：:Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901046x

    authors： Nguyen TT,Fedorov AA,Williams L,Fedorov EV,Li Y,Xu C,Almo SC,Raushel FM

    更新日期：2009-09-22 00:00:00

  • Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus.

    abstract：:Complex I of respiratory chains transfers electrons from NADH to ubiquinone, coupled to the translocation of protons across the membrane. Two alternative coupling mechanisms are being discussed, redox-driven or conformation-driven. Using "zero-length" cross-linking reagent and isolated hydrophilic domains of complex I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801160u

    authors： Berrisford JM,Thompson CJ,Sazanov LA

    更新日期：2008-09-30 00:00:00

  • Zeitlupe senses blue-light fluence to mediate circadian timing in Arabidopsis thaliana.

    abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401027n

    authors： Pudasaini A,Zoltowski BD

    更新日期：2013-10-08 00:00:00

  • Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions.

    abstract：:The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026378m

    authors： Sengupta K,Rao BJ

    更新日期：2002-12-24 00:00:00

  • Binding studies on anti-fructofuranan mouse myeloma immunoglobulins A47N, A4, U61, and E109.

    abstract：:Four murine myeloma immunoglobulins, A4, A47N, U61, and E109, have been studied for their binding affinities with inulin and a series of oligosaccharides derived from inulin. The results indicate that the combining site of these immunoglobulins shows highest complementarity for a trifructofuranosyl sequence (A4 and A4...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00636a005

    authors： Streefkerk DG,Glaudemans CP

    更新日期：1977-08-23 00:00:00

  • Synthesis and biological activity of a lambda pseudo operator.

    abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00638a013

    authors： Kawashima E,Gadek T,Caruthers MH

    更新日期：1977-09-20 00:00:00

  • Generation of autonomous activity of Ca(2+)/calmodulin-dependent protein kinase kinase β by autophosphorylation.

    abstract：:Ca(2+)/calmodulin-dependent protein kinase kinases (CaMKKs) phosphorylate and activate specific downstream protein kinases, including CaMKI, CaMKIV, and 5'-AMP-activated protein kinase, which mediates a variety of Ca(2+) signaling cascades. CaMKKs have been shown to undergo autophosphorylation, although their role in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201005g

    authors： Tokumitsu H,Hatano N,Fujimoto T,Yurimoto S,Kobayashi R

    更新日期：2011-09-27 00:00:00

  • The solution structure of Bacillus anthracis dihydrofolate reductase yields insight into the analysis of structure-activity relationships for novel inhibitors.

    abstract：:There is a significant need for new therapeutics to treat infections caused by the biodefense agent Bacillus anthracis. In pursuit of drug discovery against this organism, we have developed novel propargyl-linked inhibitors that target the essential enzyme dihydrofolate reductase (DHFR) from B. anthracis. Previously, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802319w

    authors： Beierlein JM,Deshmukh L,Frey KM,Vinogradova O,Anderson AC

    更新日期：2009-05-19 00:00:00

  • Atrazine chlorohydrolase from Pseudomonas sp. strain ADP is a metalloenzyme.

    abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020415s

    authors： Seffernick JL,McTavish H,Osborne JP,de Souza ML,Sadowsky MJ,Wackett LP

    更新日期：2002-12-03 00:00:00

  • Generation and characterization of two transgenic mouse lines expressing human ApoE2 in neurons and glial cells.

    abstract：:Apolipoprotein E (apoE) isoforms are key determinants of susceptibility to late-onset Alzheimer's disease (AD). The epsilon 4 and epsilon 2 alleles have been associated with increased and decreased risk for AD, respectively. We have generated and characterized transgenic mice in which the human apoE2 gene is expressed...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015971l

    authors： Georgopoulos S,McKee A,Kan HY,Zannis VI

    更新日期：2002-07-30 00:00:00

  • Crystal structure of arginase from Plasmodium falciparum and implications for L-arginine depletion in malarial infection .

    abstract：:The 2.15 A resolution crystal structure of arginase from Plasmodium falciparum, the parasite that causes cerebral malaria, is reported in complex with the boronic acid inhibitor 2(S)-amino-6-boronohexanoic acid (ABH) (K(d) = 11 microM). This is the first crystal structure of a parasitic arginase. Various protein const...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100390z

    authors： Dowling DP,Ilies M,Olszewski KL,Portugal S,Mota MM,Llinás M,Christianson DW

    更新日期：2010-07-06 00:00:00

  • Orientation-conserved transfer of symmetric Krebs cycle intermediates in mammalian tissue.

    abstract：:Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00186a029

    authors： Sherry AD,Sumegi B,Miller B,Cottam GL,Gavva S,Jones JG,Malloy CR

    更新日期：1994-05-24 00:00:00

  • Low-frequency dynamics of Caldariomyces fumago chloroperoxidase probed by femtosecond coherence spectroscopy.

    abstract：:Ultrafast laser spectroscopy techniques are used to measure the low-frequency vibrational coherence spectra and nitric oxide rebinding kinetics of Caldariomyces fumago chloroperoxidase (CPO). Comparisons of the CPO coherence spectra with those of other heme species are made to gauge the protein-specific nature of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7025485

    authors： Gruia F,Ionascu D,Kubo M,Ye X,Dawson J,Osborne RL,Sligar SG,Denisov I,Das A,Poulos TL,Terner J,Champion PM

    更新日期：2008-05-06 00:00:00

  • Local destabilization of the metal-binding region in human copper-zinc superoxide dismutase by remote mutations is a possible determinant for progression of ALS.

    abstract：:More than 100 distinct mutations in the gene CuZnSOD encoding human copper-zinc superoxide dismutase (CuZnSOD) have been associated with familial amyotrophic lateral sclerosis (fALS), a fatal neuronal disease. Many studies of different mutant proteins have found effects on protein stability, catalytic activity, and me...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500606j

    authors： Hennig J,Andrésen C,Museth AK,Lundström P,Tibell LA,Jonsson BH

    更新日期：2015-01-20 00:00:00

  • Statistical clustering techniques for the analysis of long molecular dynamics trajectories: analysis of 2.2-ns trajectories of YPGDV.

    abstract：:The microscopic interactions and mechanisms leading to nascent protein folding events are generally unknown. While such short time-scale events are difficult to study experimentally, molecular dynamics simulations of peptides can provide a useful model for studying events related to protein folding initiation. Recentl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00053a005

    authors： Karpen ME,Tobias DJ,Brooks CL 3rd

    更新日期：1993-01-19 00:00:00

  • A cluster of aromatic residues in the sixth membrane-spanning segment of the dopamine D2 receptor is accessible in the binding-site crevice.

    abstract：:The binding site of the dopamine D2 receptor, like that of other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using the substituted-cysteine accessibility method, we previously mapped the residues in the third, fifth, and seve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972241y

    authors： Javitch JA,Ballesteros JA,Weinstein H,Chen J

    更新日期：1998-01-27 00:00:00

  • Effect of surface curvature on stability, thermodynamic behavior, and osmotic activity of dipalmitoylphosphatidylcholine single lamellar vesicles.

    abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00515a024

    authors： Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

    更新日期：1981-06-09 00:00:00

  • Effect of mixed solvents on the formation of horseradish peroxidase compound I. The importance of diffusion-controlled reactions.

    abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00632a023

    authors： Dunford HB,Hewson WD

    更新日期：1977-06-28 00:00:00

  • Effects of S-adenosyl-1,8-diamino-3-thiooctane on polyamine metabolism.

    abstract：:Exposure of mammalian cells (transformed mouse fibroblasts or rat hepatoma cells) to S-adenosyl-1,8-diamino-3-thiooctane produced profound changes in the intracellular polyamine content. Putrescine was increased and spermidine was decreased, consistent with the inhibition of spermidine synthase by this compound, which...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00263a036

    authors： Pegg AE,Tang KC,Coward JK

    更新日期：1982-09-28 00:00:00

  • The N-terminal 1000 residues of apolipoprotein B associate with microsomal triglyceride transfer protein to create a lipid transfer pocket required for lipoprotein assembly.

    abstract：:Apolipoprotein (apo) B, the major protein component of the atherogenic low-density lipoprotein (LDL), has a pentapartite structure, NH2-betaalpha1-beta1-alpha2-beta2-alpha3-COOH, the beta domains containing multiple amphipathic beta strands and the alpha domains containing multiple amphipathic alpha helixes. We recent...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011757l

    authors： Dashti N,Gandhi M,Liu X,Lin X,Segrest JP

    更新日期：2002-06-04 00:00:00

  • Zinc site redesign in T4 gene 32 protein: structure and stability of cobalt(II) complexes formed by wild-type and metal ligand substitution mutants.

    abstract：:Phage T4 gene 32 protein (gp32) is a zinc metalloprotein which binds cooperatively and preferentially to single-stranded nucleic acids and functions as a replication and recombination accessory protein. Zn(II) coordination by gp32 employs a His-Cys3 metal ligand donor set derived from the His64-X12-Cys77-X9-Cys87-X2-C...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9617769

    authors： Guo J,Giedroc DP

    更新日期：1997-01-28 00:00:00

  • Hypertrophic cardiomyopathy-linked mutation D145E drastically alters calcium binding by the C-domain of cardiac troponin C.

    abstract：:The role of the C-domain sites of cardiac troponin C in the modulation of the calcium signal remains unclear. In this study, we investigated the effects of hypertrophic cardiomyopathy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sites. The A8V mutation had essentially ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100400h

    authors： Swindle N,Tikunova SB

    更新日期：2010-06-15 00:00:00

  • Calorimetric studies on the interaction of horse ferricytochrome c and yeast cytochrome c peroxidase.

    abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00026a022

    authors： Kresheck GC,Vitello LB,Erman JE

    更新日期：1995-07-04 00:00:00

  • In the sense of transcription regulation by G-quadruplexes: asymmetric effects in sense and antisense strands.

    abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401451q

    authors： Agarwal T,Roy S,Kumar S,Chakraborty TK,Maiti S

    更新日期：2014-06-17 00:00:00

  • Properties of membrane-inserted protein kinase C.

    abstract：:Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00420a003

    authors： Bazzi MD,Nelsestuen GL

    更新日期：1988-10-04 00:00:00

  • His86 from the N-terminus of frataxin coordinates iron and is required for Fe-S cluster synthesis.

    abstract：:Human frataxin has a vital role in the biosynthesis of iron-sulfur (Fe-S) clusters in mitochondria, and its deficiency causes the neurodegenerative disease Friedreich's ataxia. Proposed functions for frataxin in the Fe-S pathway include iron donation to the Fe-S cluster machinery and regulation of cysteine desulfurase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400443n

    authors： Gentry LE,Thacker MA,Doughty R,Timkovich R,Busenlehner LS

    更新日期：2013-09-03 00:00:00

  • Efficient repair of 8-oxo-7,8-dihydrodeoxyguanosine in human and hamster xeroderma pigmentosum D cells.

    abstract：:The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992610l

    authors： Cappelli E,Degan P,Thompson LH,Frosina G

    更新日期：2000-08-29 00:00:00

  • Flavin conformational changes in the catalytic cycle of p-hydroxybenzoate hydroxylase substituted with 6-azido- and 6-aminoflavin adenine dinucleotide.

    abstract：:Crystallographic studies have demonstrated two flavin conformations for p-hydroxybenzoate hydroxylase (PHBH) [Gatti, D. L., Palfey, B. A. , Lah, M. S., Entsch, B., Massey, V., Ballou, D. P., & Ludwig, M. L. (1994) Science 266, 110-114. Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971427u

    authors： Palfey BA,Ballou DP,Massey V

    更新日期：1997-12-16 00:00:00

  • The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.

    abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049127h

    authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

    更新日期：2004-08-17 00:00:00