Chemical modification of mitochondrial transhydrogenase: evidence for two classes of sulfhydryl groups.

abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...

journal_title：Biochemistry

authors： Lee R,Kaushik N,Modak MJ,Vinayak R,Pandey VN

更新日期：1998-01-20 00:00:00

abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

journal_title：Biochemistry

authors： Swaminathan N,Braunstein GD

更新日期：1978-12-26 00:00:00

abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

journal_title：Biochemistry

authors： Cech CL,McClure WR

更新日期：1980-05-27 00:00:00

abstract：:The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...

journal_title：Biochemistry

authors： Joo SH,Chung HS,Raetz CR,Garrett TA

更新日期：2012-05-29 00:00:00

abstract：:In order to understand translocation in transcription, it is important to develop a continuous functional assay for RNA polymerase (RNAP) activity in vitro. Fluorescent derivatives of ATP, UTP, UpA, and CpA with aminonaphthalene-5-sulfonic acid (AmNS) attached to the nucleotide triphosphates via a gamma-phosphoramidat...

journal_title：Biochemistry

authors： Tyagi SC,Wu FY

更新日期：1992-07-21 00:00:00

abstract：:The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...

journal_title：Biochemistry

authors： Ishimori K,Tsuneshige A,Imai K,Morishima I

更新日期：1989-10-17 00:00:00

abstract：:Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...

journal_title：Biochemistry

authors： Li Z,Pal B,Takenaka S,Tsuyama S,Kitagawa T

更新日期：2005-01-25 00:00:00

abstract：:Unfolded apomyoglobin in 8 M urea at pH 2.3 displays distinct regions with different backbone mobility, as monitored by NMR relaxation. These variations in backbone mobility can be correlated with intrinsic properties of the amino acids in the sequence. Clusters of small amino acids such as glycine and alanine show in...

journal_title：Biochemistry

authors： Schwarzinger S,Wright PE,Dyson HJ

更新日期：2002-10-22 00:00:00

abstract：:In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...

journal_title：Biochemistry

authors： McCormick MS,Lippard SJ

更新日期：2011-12-27 00:00:00

abstract：:Iron K-edge X-ray spectroscopy (XANES and EXAFS) was used to study iron coordination in frozen solutions of soybean lipoxygenase-1 (SLO). The intensity of the 1s-->3d pre-edge transition of native iron(II) lipoxygenase is greater than what was found for six-coordinate high-spin iron(II) model complexes, but comparable...

journal_title：Biochemistry

authors： Scarrow RC,Trimitsis MG,Buck CP,Grove GN,Cowling RA,Nelson MJ

更新日期：1994-12-20 00:00:00

abstract：:The phosphorylation mechanism of Dictyostelium discoideum nucleoside diphosphate (NDP) kinase was investigated by NMR. 31P chemical shifts were measured on both native and denatured enzyme. In the enzymatically phosphorylated enzyme denatured by 9 M urea or 7 M guanidine hydrochloride, the NDP kinase phosphohistidine ...

journal_title：Biochemistry

authors： Lecroisey A,Lascu I,Bominaar A,Véron M,Delepierre M

更新日期：1995-09-26 00:00:00

abstract：:We have designed a novel estrogen-responsive unit, overERE, which consists of two overlapping ERE separated by 5 bp (center-to-center). In gel retardation assays, this sequence forms a low-mobility complex that migrates like an estrogen receptor tetramer. The receptor-overERE complex was specific and was supershifted ...

journal_title：Biochemistry

authors： Massaad C,Coumoul X,Sabbah M,Garlatti M,Redeuilh G,Barouki R

更新日期：1998-04-28 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...

journal_title：Biochemistry

authors： Clausen H,McKibbin JM,Hakomori S

更新日期：1985-10-22 00:00:00

abstract：:The motion of the ethanolamine head group in unsonicated lipid bilayers above and below the phase transition is studied by means of deuterium and phosphorus magnetic resonance. For this purpose, dipalmitoyl-3-sn-phosphatidylethanolamine is selectively deuterated at the two ethanolamine carbon atoms. The deuterium quad...

journal_title：Biochemistry

authors： Seelig J,Gally H

更新日期：1976-11-30 00:00:00

abstract：:N-(1-Pyrenyl)iodoacetamide has been used to introduce fluorescent probes into Escherichia coli RNA polymerase. After an incubation time of 15 min, approximately 2 pyrene equiv was introduced per enzyme molecule. There was no further increase in modification after more extended periods of incubation. Neither calf thymu...

journal_title：Biochemistry

authors： Johnson RS,Bowers M,Eaton Q

更新日期：1991-01-08 00:00:00

abstract：:The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...

journal_title：Biochemistry

authors： Igloi GL,von der Haar F,Cramer F

更新日期：1978-08-22 00:00:00

abstract：:The near-ultraviolet circular dichroism (CD) of three homogeneous anti-type III pneumococcal antibodies in the absence and the presence of the specific hexasaccharide ligand was studied. In addition recombinations and hybridizations of H and L chains derived from two of these antibodies were carried out and the CD spe...

journal_title：Biochemistry

authors： Jaton JC,Huser H,Blatt Y,Pecht I

更新日期：1975-12-02 00:00:00

abstract：:Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appea...

journal_title：Biochemistry

authors： Silow M,Tan YJ,Fersht AR,Oliveberg M

更新日期：1999-10-05 00:00:00

abstract：:The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer ...

journal_title：Biochemistry

authors： Wei TF,Bujalowski W,Lohman TM

更新日期：1992-07-07 00:00:00

abstract：:Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...

journal_title：Biochemistry

authors： Mursinna RS,Lincecum TL Jr,Martinis SA

更新日期：2001-05-08 00:00:00

abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

journal_title：Biochemistry

authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

更新日期：2000-02-22 00:00:00

abstract：:The substrate profiles for three uncharacterized enzymes (YcjM, YcjT, and YcjU) that are expressed from a cluster of 12 genes ( ycjM-W and ompG) of unknown function in Escherichia coli K-12 were determined. Through a comprehensive bioinformatic and steady-state kinetic analysis, the catalytic function of YcjT was dete...

journal_title：Biochemistry

authors： Mukherjee K,Narindoshvili T,Raushel FM

更新日期：2018-05-15 00:00:00

abstract：:Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...

journal_title：Biochemistry

authors： Fujikawa K,Walsh KA,Davie EW

更新日期：1977-05-17 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probe...

journal_title：Biochemistry

authors： Shao J,Cieslak J,Gross A

更新日期：2009-01-27 00:00:00

abstract：:As part of our studies on the molecular mechanism of mutation [Chambers, R. W. (1982) in Molecular and Cellular Mechanisms of Mutagenesis (Lemontt, J. F., & Generoso, W. M., Eds.) pp 121-145, Plenum, New York and London], we wanted to prepare specific oligonucleotides carrying O2- or O4-alkylthymidine residues. Since ...

journal_title：Biochemistry

authors： Borowy-Borowski H,Chambers RW

更新日期：1989-02-21 00:00:00

abstract：:Allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. Several observations suggested that the usual allosteric mechanism might not apply to the activation of ...

journal_title：Biochemistry

authors： Riera TV,Zheng L,Josephine HR,Min D,Yang W,Hedstrom L

更新日期：2011-10-04 00:00:00

abstract：:FeNO vibrational frequencies were investigated for a series of myoglobin mutants using isotope-edited resonance Raman spectra of (15/14)NO adducts, which reveal the FeNO and NO stretching modes. The latter give rise to doublet bands, as a result of Fermi resonances with coincident porphyrin vibrations; these doublets ...

journal_title：Biochemistry

authors： Coyle CM,Vogel KM,Rush TS 3rd,Kozlowski PM,Williams R,Spiro TG,Dou Y,Ikeda-Saito M,Olson JS,Zgierski MZ

更新日期：2003-05-06 00:00:00

abstract：:We have covalently attached an electron paramagnetic resonance (EPR) spin probe to Cys-670 of the motor domain of ncd (nonclaret disjunctional protein) in order to investigate conformational changes associated with the chemomechanical cycle. Spin-labeling is highly specific and does not affect ncd function as monitore...

journal_title：Biochemistry

authors： Naber N,Cooke R,Pate E

更新日期：1997-08-12 00:00:00