Unesterified long chain fatty acids inhibit the binding of single chain urokinase to the urokinase receptor.

abstract：:NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active asse...

journal_title：Biochemistry

authors： Barbier P,Peyrot V,Leynadier D,Andreu JM

更新日期：1998-01-13 00:00:00

abstract：:We reported previously that cdc2 kinase decreased the microtubule-stabilizing ability of a major HeLa cell microtubule-associated protein, MAP4, by phosphorylation in vitro [Ookata, K., et al. (1995) J. Cell Biol. 128, 849-862]. An important question raised by this study is whether MAP4 is indeed phosphorylated by cdc...

journal_title：Biochemistry

authors： Ookata K,Hisanaga S,Sugita M,Okuyama A,Murofushi H,Kitazawa H,Chari S,Bulinski JC,Kishimoto T

更新日期：1997-12-16 00:00:00

abstract：:The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alky...

journal_title：Biochemistry

authors： Jo H,Culik RM,Korendovych IV,Degrado WF,Gai F

更新日期：2010-12-14 00:00:00

abstract：:Stimulation of intracellular cholesterol esterification, which is catalyzed by the enzyme acyl-CoA:cholesterol O-acyltransferase (ACAT), by atherogenic lipoproteins in macrophages is a key step in the development of atheroma foam cells. Since other aspects of intracellular cholesterol metabolism involve proteolytic re...

journal_title：Biochemistry

authors： Schissel SL,Beatini N,Zha X,Maxfield FR,Tabas I

更新日期：1995-08-22 00:00:00

abstract：:Caveolae are approximately 50-100 nm invaginations of the plasma membrane thought to form as a result of a local accumulation of cholesterol, sphingolipids, and a unique family of three proteins known as the caveolins: Cav-1, -2, and -3. Here, we report the identification, sequence, and developmental expression of the...

journal_title：Biochemistry

authors： Razani B,Park DS,Miyanaga Y,Ghatpande A,Cohen J,Wang XB,Scherer PE,Evans T,Lisanti MP

更新日期：2002-06-25 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at differe...

journal_title：Biochemistry

authors： Thompson JF,Bachellerie JP,Hall K,Hearst JE

更新日期：1982-03-16 00:00:00

abstract：:Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify ...

journal_title：Biochemistry

authors： Wu EY,Walsh AR,Materne EC,Hiltner EP,Zielinski B,Miller BR 3rd,Mawby L,Modeste E,Parish CA,Barnes WM,Kermekchiev MB

更新日期：2015-01-27 00:00:00

abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...

journal_title：Biochemistry

authors： Xing H,Mattick S,Lew D,Shapiro DJ

更新日期：1995-03-28 00:00:00

abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...

journal_title：Biochemistry

authors： Wijffels G,Dalrymple BP,Prosselkov P,Kongsuwan K,Epa VC,Lilley PE,Jergic S,Buchardt J,Brown SE,Alewood PF,Jennings PA,Dixon NE

更新日期：2004-05-18 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:The oxycomplexes (compound III, oxyperoxidase) of two lignin peroxidase isozymes, H1 (pI = 4.7) and H8 (pI = 3.5), were characterized in the present study. After generation of the ferroperoxidase by photochemical reduction with deazoflavin in the presence of EDTA, the oxycomplex is formed by mixing ferroperoxidase wit...

journal_title：Biochemistry

authors： Cai DY,Tien M

更新日期：1990-02-27 00:00:00

abstract：:Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...

journal_title：Biochemistry

authors： Kizer M,Huntress ID,Walcott BD,Fraser K,Bystroff C,Wang X

更新日期：2019-03-12 00:00:00

abstract：:The downfield (9-15 ppm) proton spectrum of Escherichia coli 5S RNA has been examined at 500 MHz by using nuclear Overhauser methods. The data confirm the existence of the terminal and procaryotic loop helices within the molecule [Fox, G. E., & Woese, C. R. (1975) Nature (London) 256, 505-506]. Very little stable, dou...

journal_title：Biochemistry

authors： Kime MJ,Moore PB

更新日期：1983-05-24 00:00:00

abstract：:Purified human von Willebrand factor (vWF) was digested with Staphylococcus aureus V-8 protease, and specific domains interacting with platelets were isolated and characterized. Amino acid sequence analysis and sodium dodecyl sulfate gel electrophoresis demonstrated that the digestion proceeded primarily by a single c...

journal_title：Biochemistry

authors： Girma JP,Chopek MW,Titani K,Davie EW

更新日期：1986-06-03 00:00:00

abstract：:Resonance Raman vibrational spectra of the Pr, lumi-R, and Pfr forms of phytochrome have been obtained using low-temperature trapping and room temperature flow techniques in conjunction with shifted-excitation Raman difference spectroscopy (SERDS). The Pr to lumi-R photoconversion exhibits a thermal barrier and is com...

journal_title：Biochemistry

authors： Andel F 3rd,Lagarias JC,Mathies RA

更新日期：1996-12-17 00:00:00

abstract：:Traditionally, assigning the heme protein resonances has relied heavily on the comparison of spectra arising from protein reconstituted with specifically deuterated hemes and the native form. Such an approach can identify tentatively the broad, overlapping signals in the Fe(II) high-spin heme protein spectra. Although...

journal_title：Biochemistry

authors： Busse SC,Jue T

更新日期：1994-09-13 00:00:00

abstract：:High signal-to-noise Fourier transform infrared (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT(3)R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanog...

journal_title：Biochemistry

authors： Rigler P,Ulrich WP,Hovius R,Ilegems E,Pick H,Vogel H

更新日期：2003-12-02 00:00:00

abstract：:We investigated the effects of MA-5, a human-specific monoclonal antibody to the insulin receptor alpha-subunit, on transmembrane signaling in cell lines transfected with and expressing both normal human insulin receptors and receptors mutated in their beta-subunit tyrosine kinase domains. In cell lines expressing nor...

journal_title：Biochemistry

authors： Sung CK,Han XL,Brunetti A,Maddux B,Yamamoto-Honda R,Goldfine ID

更新日期：1992-01-14 00:00:00

abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

journal_title：Biochemistry

authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

更新日期：1995-10-17 00:00:00

abstract：:In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...

journal_title：Biochemistry

authors： Jefferies D,Hsu PC,Khalid S

更新日期：2017-03-21 00:00:00

abstract：:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...

journal_title：Biochemistry

authors： Mori E,Takasaki S,Hedrick JL,Wardrip NJ,Mori T,Kobata A

更新日期：1991-02-26 00:00:00

abstract：:Prion proteins (PrP) are the infectious agent in transmissible spongiform encephalopathies (i.e., mad cow disease). To be infectious, prion proteins must undergo a conformational change involving a decrease in α-helical content along with an increase in β-strand content. This conformational change was evaluated by mea...

journal_title：Biochemistry

authors： Samson AO,Levitt M

更新日期：2011-03-29 00:00:00

abstract：:The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonat...

journal_title：Biochemistry

authors： O'Brien JR,Schuller DJ,Yang VS,Dillard BD,Lanzilotta WN

更新日期：2003-05-20 00:00:00

abstract：:Membrane fusion induced by the hemagglutinin glycoprotein of influenza virus has been extensively characterized, but the mechanism whereby the protein achieves the merger of the viral and target membrane lipids remains enigmatic. Various lipid intermediate structures have been proposed, and the energies required for t...

journal_title：Biochemistry

authors： Nebel S,Bartoldus I,Stegmann T

更新日期：1995-05-02 00:00:00

abstract：:Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...

journal_title：Biochemistry

authors： Wang H,Shu Q,Frieden C,Gross ML

更新日期：2017-06-13 00:00:00

abstract：:Helicases are enzymes that use energy from nucleoside triphosphate hydrolysis to unwind double-stranded (ds) DNA, a process vital to virtually every phase of DNA metabolism. Helicases have been classified as either 5'-to-3' or 3'-to-5' on the basis of their ability to unwind duplex DNA adjacent to either a 5' or 3' si...

journal_title：Biochemistry

authors： Morris PD,Byrd AK,Tackett AJ,Cameron CE,Tanega P,Ott R,Fanning E,Raney KD

更新日期：2002-02-19 00:00:00

abstract：:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA)...

journal_title：Biochemistry

authors： Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NT

更新日期：1986-11-04 00:00:00

abstract：:SHP-1, a protein tyrosine phosphatase containing two tandem SH2 domains, is autoinhibited at rest by its N-terminal SH2 (N-SH2) domain. Relief from autoinhibition and a subsequent 10-60-fold increase in V(max) have been observed upon N-SH2 domain engagement by a specific phosphotyrosyl ligand or upon deletion of the S...

journal_title：Biochemistry

authors： Wang J,Walsh CT

更新日期：1997-03-11 00:00:00

abstract：:Adenylosuccinate lyase, an enzyme catalyzing two reactions in purine biosynthesis (the cleavage of either adenylosuccinate or succinylaminoimidazole carboxamide ribotide), has been implicated in a human disease arising from point mutations in the gene encoding the enzyme. Asn(276) of Bacillus subtilis adenylosuccinate...

journal_title：Biochemistry

authors： Palenchar JB,Colman RF

更新日期：2003-02-25 00:00:00