当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Effects of exposure to low pH on the lateral mobility of influenza hemagglutinin expressed at the cell surface: correlation between mobility inhibition and inactivation.

Effects of exposure to low pH on the lateral mobility of influenza hemagglutinin expressed at the cell surface: correlation between mobility inhibition and inactivation.

Abstract：

:To investigate the possible role of viral glycoprotein mobility in membrane fusion, fluorescence photobleaching recovery was employed to study the effects of exposure to mildly acidic pH (required to convert many viral fusion proteins to the fusion-active form) on the lateral mobility of influenza hemagglutinin (HA) proteins expressed at the surface of transfected cells. HA proteins from two different strains were compared: X:31 HA, which is activated by a brief exposure to pH 4.9 but is irreversibly inactivated at longer exposure times, and HA from A/Japan/305/57, which is relatively stable to inactivation at this pH [Puri, A., Booy, F.P., Doms, R.W., White, J.M., & Blumenthal, R. (1990) J. Virol. 64, 3824-3832]. The HA proteins from both strains, expressed in CV-1 cells using VS-40 vectors, exhibited relatively unrestricted lateral diffusion at the cell surface. The high mobility persisted following a brief exposure (1 min) to pH 4.9 to mediate conversion to the fusogenic state. Longer times (up to 15 min) of preincubation at pH 4.9 inhibited the lateral mobility of X:31 HA (the lateral diffusion rate was markedly reduced, followed by immobilization) but not of A/Japan HA, whose fusion activity is resistant to such treatment. Inhibition of the lateral mobility of X:31 HA due to preincubation at low pH was not specific to the CV-1 cells and was found also in a CHO cell line stably expressing this protein. The results presented demonstrate a close correlation between loss of mobility and inactivation of fusogenic activity, in accord with the notion that lateral motion of the HA proteins is required for fusion.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gutman O,Danieli T,White JM,Henis YI

doi

10.1021/bi00052a014

subject

Has Abstract

pub_date

1993-01-12 00:00:00

pages

101-6

issue

1

eissn

0006-2960

issn

1520-4995

journal_volume

32

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Native-like folding intermediates of homologous ribonucleases.

    abstract：:The mechanism of the slow refolding reactions of four different pancreatic ribonucleases from ox, sheep, red deer, and roe deer has been investigated. Refolding kinetics of these proteins were very similar. In particular, a native-like intermediate, IN, was shown to be populated on the slow refolding pathway of all ri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00336a005

    authors： Krebs H,Schmid FX,Jaenicke R

    更新日期：1985-07-16 00:00:00

  • Role of the cochaperone Tpr2 in Hsp90 chaperoning.

    abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800770g

    authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

    更新日期：2008-08-05 00:00:00

  • Spatial proximity of the glycine-rich loop and the SH2 thiol in myosin subfragment 1.

    abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00408a045

    authors： Sutoh K,Hiratsuka T

    更新日期：1988-04-19 00:00:00

  • The Q-loop of DrrA is involved in producing the closed conformation of the nucleotide binding domains and in transduction of conformational changes between DrrA and DrrB.

    abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701699a

    authors： Rao DK,Kaur P

    更新日期：2008-03-04 00:00:00

  • Structure-Related Roles for the Conservation of the HIV-1 Fusion Peptide Sequence Revealed by Nuclear Magnetic Resonance.

    abstract：:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00745

    authors： Serrano S,Huarte N,Rujas E,Andreu D,Nieva JL,Jiménez MA

    更新日期：2017-10-17 00:00:00

  • 1H nuclear magnetic resonance double resonance study of oxytocin in aqueous solution.

    abstract：:Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00650a025

    authors： Glickson JD,Rowan R,Pitner TP,Dadok J,Bothner-By AA,Walter R

    更新日期：1976-03-09 00:00:00

  • Effects of the N-terminal acylamido group of imidazole- and pyrrole-containing polyamides on DNA sequence specificity and binding affinity.

    abstract：:The N-terminal formamido group on imidazole- and pyrrole-containing polyamides causes stacked polyamides to bind in the minor groove of DNA in the staggered motif, and it also increases the binding affinity compared to those of non-formamido compounds. To further investigate the role of the N-terminal acylamido in aff...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900242t

    authors： Westrate L,Mackay H,Brown T,Nguyen B,Kluza J,Wilson WD,Lee M,Hartley JA

    更新日期：2009-06-23 00:00:00

  • Substrate-Induced Carbon Monoxide Reactivity Suggests Multiple Enzyme Conformations at the Catalytic Copper M-Center of Peptidylglycine Monooxygenase.

    abstract：:The present study uses CO as a surrogate for oxygen to probe how substrate binding triggers oxygen activation in peptidylglycine monooygenase (PHM). Infrared stretching frequencies (ν(C ≡ O)) of the carbonyl (CO) adducts of copper proteins are sensitive markers of Cu(I) coordination and are useful in probing oxygen re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00845

    authors： Kline CD,Blackburn NJ

    更新日期：2016-12-06 00:00:00

  • Refolding of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase in the presence of ligand: a stopped-flow NMR spectroscopy study.

    abstract：:Escherichia coli dihydrofolate reductase contains five tryptophan residues that are spatially distributed throughout the protein and located in different secondary structural elements. When these tryptophans are replaced with [6-19F]tryptophan, distinct native and unfolded resonances can be resolved in the 1-D 19F NMR...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971962u

    authors： Hoeltzli SD,Frieden C

    更新日期：1998-01-06 00:00:00

  • Allosteric Modulation of JNK Docking Site Interactions with ATP-Competitive Inhibitors.

    abstract：:The c-Jun N-terminal kinases (JNKs) play a wide variety of roles in cellular signaling processes, dictating important, and even divergent, cellular fates. These essential kinases possess docking surfaces distal to their active sites that interact with diverse binding partners, including upstream activators, downstream...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00776

    authors： Lombard CK,Davis AL,Inukai T,Maly DJ

    更新日期：2018-10-09 00:00:00

  • Differential susceptibility of transferrin glycoforms to chymotrypsin: a proteomics approach to the detection of carbohydrate-deficient transferrin.

    abstract：:Transferrin exhibits heterogeneity in glycosylation characteristic of pathological changes in alcohol abuse and congenital disorders in glycosylation. This study investigated an alternative approach in the detection of carbohydrate-deficient transferrin based on the premise that glycosylation may afford some degree of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051749v

    authors： Valmu L,Kalkkinen N,Husa A,Rye PD

    更新日期：2005-12-13 00:00:00

  • The diheme cytochrome c(4) from Vibrio cholerae is a natural electron donor to the respiratory cbb(3) oxygen reductase.

    abstract：:The respiratory chain of Vibrio cholerae contains three bd-type quinol oxygen reductases as well as one cbb(3) oxygen reductase. The cbb(3) oxygen reductase has been previously isolated and characterized; however, the natural mobile electron donor(s) that shuttles electrons between the bc(1) complex and the cbb(3) oxy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1004574

    authors： Chang HY,Ahn Y,Pace LA,Lin MT,Lin YH,Gennis RB

    更新日期：2010-09-07 00:00:00

  • Identification of residues in CD6 which are critical for ligand binding.

    abstract：:CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962560+

    authors： Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

    更新日期：1997-03-04 00:00:00

  • Prion-like p53 Amyloids in Cancer.

    abstract：:The global transcription factor, p53, is a master regulator of gene expression in cells. Mutations in the TP53 gene promote unregulated cell growth through the inactivation of downstream effectors of the p53 pathway. In fact, mutant p53 is highly prone to misfolding and frequently resides inside the cell as large aggr...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/acs.biochem.9b00796

    authors： Navalkar A,Ghosh S,Pandey S,Paul A,Datta D,Maji SK

    更新日期：2020-01-21 00:00:00

  • Folding and domain-domain interactions of the chaperone PapD measured by 19F NMR.

    abstract：:The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048614u

    authors： Bann JG,Frieden C

    更新日期：2004-11-02 00:00:00

  • Analysis of membrane-binding properties of dermaseptin analogues: relationships between binding and cytotoxicity.

    abstract：:To understand relationships between membrane-binding properties of cytolytic peptides and resulting cytotoxicity, we investigated interactions of dermaseptin analogues with model bilayers by means of surface plasmon resonance. First, we tested the system by comparing two native dermaseptins, S1 and S4, whose binding p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034514x

    authors： Gaidukov L,Fish A,Mor A

    更新日期：2003-11-11 00:00:00

  • Pinpoint chemical modification of Asp160 in the 49 kDa subunit of bovine mitochondrial complex I via a combination of ligand-directed tosyl chemistry and click chemistry.

    abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501342w

    authors： Masuya T,Murai M,Morisaka H,Miyoshi H

    更新日期：2014-12-16 00:00:00

  • A Ca2+-activated protease possibly involved in myofibrillar protein turnover. Purification from porcine muscle.

    abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00655a019

    authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

    更新日期：1976-05-18 00:00:00

  • cAMP binds with high affinity and specificity to the androgen receptor from murine skeletal muscle.

    abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00423a013

    authors： Ofenloch-Hähnle B,Eisele K

    更新日期：1988-11-15 00:00:00

  • Sterol and pH interdependence in the binding, oligomerization, and pore formation of Listeriolysin O.

    abstract：:Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602497g

    authors： Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

    更新日期：2007-04-10 00:00:00

  • Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate.

    abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000855o

    authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

    更新日期：2000-09-05 00:00:00

  • An all-D amino acid peptide model of alpha1(IV)531-543 from type IV collagen binds the alpha3beta1 integrin and mediates tumor cell adhesion, spreading, and motility.

    abstract：:Type IV collagen promotes integrin-mediated cell adhesion, spreading, and motility. Several regions within the triple-helical domain of type IV collagen have been identified as tumor cellular recognition sites. Among these regions, the alpha1(IV)531-543 sequence, designated L-Hep-III, promotes integrin-mediated tumor ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971817g

    authors： Li C,McCarthy JB,Furcht LT,Fields GB

    更新日期：1997-12-09 00:00:00

  • Albumin-bound quercetin repairs vitamin E oxidized by apolipoprotein radicals in native HDL3 and LDL.

    abstract：:In the minor fraction of HDL3 containing alpha-tocopherol (alphaTocOH), selective one-electron oxidation of Trp and Tyr residues of apolipoproteins A-I and A-II by *Br2- radical-anions produces the corresponding semioxidized species, TyrO* and *Trp. Repair of TyrO* by endogenous alphaTocOH generates the alpha-tocopher...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701419d

    authors： Filipe P,Patterson LK,Bartels DM,Hug GL,Freitas JP,Mazière JC,Santus R,Morlière P

    更新日期：2007-12-11 00:00:00

  • Human CD4 Metastability Is a Function of the Allosteric Disulfide Bond in Domain 2.

    abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00154

    authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

    更新日期：2016-04-19 00:00:00

  • The crystal structure of the Escherichia coli maltodextrin phosphorylase-acarbose complex.

    abstract：:Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9828573

    authors： O'Reilly M,Watson KA,Johnson LN

    更新日期：1999-04-27 00:00:00

  • Activation of overexpressed receptors for insulin and epidermal growth factor interferes in mitogenic signaling without affecting the activation of p21ras.

    abstract：:Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00189a053

    authors： Osterop AP,Medema RH,Ouwens DM,Van der Zon GC,Möller W,Maassen JA

    更新日期：1994-06-14 00:00:00

  • The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.

    abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00047a003

    authors： Barak LS,Ménard L,Ferguson SS,Colapietro AM,Caron MG

    更新日期：1995-11-28 00:00:00

  • High-efficiency triple-helix-mediated photo-cross-linking at a targeted site within a selectable mammalian gene.

    abstract：:Targeting damage to specific sites in the genome represents an attractive approach to manipulating gene function in mammalian cells. To test the applicability of triple-helix formation as a means for achieving precisely timed site-specific damage within a mammalian gene, a triplex-forming oligodeoxyribonucleotide (TFO...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960881f

    authors： Vasquez KM,Wensel TG,Hogan ME,Wilson JH

    更新日期：1996-08-20 00:00:00

  • Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering.

    abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00719

    authors： Tong X,Li T,Li C,Wang LX

    更新日期：2018-09-04 00:00:00

  • Calmodulin-specific monoclonal antibodies inhibit DNA replication in mammalian cells.

    abstract：:The involvement of calmodulin in the proliferation of Chinese hamster embryo fibroblast cells has been studied with a specific monoclonal antibody to calmodulin. We observed that calmodulin levels increase 2-fold in the late G1 period in these cells, and this coincides with the increase in DNA polymerase alpha activit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00158a002

    authors： Reddy GP,Reed WC,Sheehan E,Sacks DB

    更新日期：1992-11-03 00:00:00