Heme Trafficking and Modifications during System I Cytochrome c Biogenesis: Insights from Heme Redox Potentials of Ccm Proteins.

abstract：:The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...

journal_title：Biochemistry

authors： Juszczak LJ,Desamero RZ

更新日期：2009-03-31 00:00:00

abstract：:Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...

journal_title：Biochemistry

authors： Wu WI,Carman GM

更新日期：1996-03-26 00:00:00

abstract：:A two-step pathway consisting of an acyl-acyl carrier protein (ACP) reductase (AAR) and an aldehyde-deformylating oxygenase (ADO) allows various cyanobacteria to convert long-chain fatty acids into hydrocarbons. AAR catalyzes the two-electron, NADPH-dependent reduction of a fatty acid attached to ACP via a thioester l...

journal_title：Biochemistry

authors： Warui DM,Pandelia ME,Rajakovich LJ,Krebs C,Bollinger JM Jr,Booker SJ

更新日期：2015-02-03 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...

journal_title：Biochemistry

authors： Ahmed SA,McPhie P,Smith LA

更新日期：2003-11-04 00:00:00

abstract：:The rigor complexes that formed between rabbit skeletal muscle F-actin and chicken gizzard heavy meromyosin (HMM), in which the heavy chains had been cleaved with trypsin into 24K, 50K, and 68K fragments, were examined by using the zero-length chemical cross-linker 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC)...

journal_title：Biochemistry

authors： Onishi H,Maita T,Matsuda G,Fujiwara K

更新日期：1989-02-21 00:00:00

abstract：:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting...

journal_title：Biochemistry

authors： Anderson ET,Fletcher L,Severin A,Murphy E,Baker SM,Matsuka YV

更新日期：2004-09-21 00:00:00

abstract：:The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

journal_title：Biochemistry

authors： Falcon CM,Matthews KS

更新日期：2001-12-25 00:00:00

abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...

journal_title：Biochemistry

authors： Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

更新日期：2000-11-28 00:00:00

abstract：:The fusion of different protein domains via peptide linkers is a powerful, modular approach to obtain proteins with new functions. A detailed understanding of the conformational behavior of peptide linkers is important for applications such as fluorescence resonance energy transfer (FRET)-based sensor proteins and mul...

journal_title：Biochemistry

authors： Evers TH,van Dongen EM,Faesen AC,Meijer EW,Merkx M

更新日期：2006-11-07 00:00:00

abstract：:In this paper, we examined the posttranslational modifications (PTMs) of high-mobility group A1 (HMGA1) proteins in PC-3 human prostate cancer cells that are either treated or not treated with a histone deacetylase inhibitor, sodium butyrate. We found that, from a reversed-phase C4 column, the HMGA1a protein eluted in...

journal_title：Biochemistry

authors： Jiang X,Wang Y

更新日期：2006-06-13 00:00:00

abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...

journal_title：Biochemistry

authors： Ishihara K,Suzuki J,Nagata S

更新日期：2016-06-14 00:00:00

abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

journal_title：Biochemistry

authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

更新日期：2008-08-05 00:00:00

abstract：:Increasing efforts are being invested in the construction of nanostructures with desired shapes and physical and chemical properties. Our strategy involves nanostructure design using naturally occurring protein building blocks. Inspection of the protein structural database (PDB) reveals the richness of the conformatio...

journal_title：Biochemistry

authors： Zheng J,Zanuy D,Haspel N,Tsai CJ,Alemán C,Nussinov R

更新日期：2007-02-06 00:00:00

abstract：:One-carbon units are essential to a variety of anabolic processes which yield necessary cellular components including purines, pyrimidines, amino acids, and lipids. Serine hydroxymethyltransferase (SHMT) is the major provider of one-carbon units in the cell. The other product of this reaction is glycine. Both of these...

journal_title：Biochemistry

authors： Kastanos EK,Woldman YY,Appling DR

更新日期：1997-12-02 00:00:00

abstract：:Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...

journal_title：Biochemistry

authors： Moreno E,Teneberg S,Adar R,Sharon N,Karlsson KA,Angström J

更新日期：1997-04-15 00:00:00

abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

journal_title：Biochemistry

authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

更新日期：1998-02-24 00:00:00

abstract：:The molecular organization of sterols in liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at 37 degrees C is examined by utilizing the fluorescent analogue of cholesterol cholesta-5,7,9-trien-3 beta-ol (cholestatrienol). (1) Cholestatrienol is shown to be indistinguishable from native cholesterol i...

journal_title：Biochemistry

authors： Hyslop PA,Morel B,Sauerheber RD

更新日期：1990-01-30 00:00:00

abstract：:Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) o...

journal_title：Biochemistry

authors： Avitabile F,Alfano C,Spadaccini R,Crescenzi O,D'Ursi AM,D'Alessio G,Tancredi T,Picone D

更新日期：2003-07-29 00:00:00

abstract：:We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...

journal_title：Biochemistry

authors： Dibrov P,Young PG,Fliegel L

更新日期：1998-06-09 00:00:00

abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...

journal_title：Biochemistry

authors： Kohno T,Kobayashi K,Maeda T,Sato K,Takashima A

更新日期：1996-12-17 00:00:00

abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

journal_title：Biochemistry

authors： Kretzschmar KM,Mendelson RA,Morales MF

更新日期：1978-06-13 00:00:00

abstract：:Phosphoprotein particles were isolated in their native state from the physiological fluid of the estuarine clam Rangia cuneata , and the characteristics of the mineral ion-protein complex which constitutes the native particle were investigated by using mineral ion binding and mineral ion exchange techniques. The parti...

journal_title：Biochemistry

authors： Marsh ME,Sass RL

更新日期：1984-03-27 00:00:00

abstract：:Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...

journal_title：Biochemistry

authors： van der Kamp MW,Sirirak J,Żurek J,Allemann RK,Mulholland AJ

更新日期：2013-11-12 00:00:00

abstract：:Serum paraoxonase hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. High serum paraoxonase levels appear to protect against the neurotoxic effects of organophosphorus substrates of this enzyme [Costa et al. (1990) Toxicol. Appl. Pharmacol. 103, 66-76]. The amino acid sequence accounting f...

journal_title：Biochemistry

authors： Hassett C,Richter RJ,Humbert R,Chapline C,Crabb JW,Omiecinski CJ,Furlong CE

更新日期：1991-10-22 00:00:00

abstract：:Carbonic anhydrase II deficiency syndrome or Marble brain disease (MBD) is caused by autosomal recessive mutations in the human carbonic anhydrase II (HCA II) gene. Here we report a small-molecule stabilization study of the exceptionally destabilized HCA II mutant H107Y employing inhibitors based on p-aminobenzoylsulf...

journal_title：Biochemistry

authors： Almstedt K,Rafstedt T,Supuran CT,Carlsson U,Hammarström P

更新日期：2009-06-16 00:00:00

abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

journal_title：Biochemistry

authors： Pancoska P,Keiderling TA

更新日期：1991-07-16 00:00:00

abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...

journal_title：Biochemistry

authors： Mercurio FA,Marasco D,Pirone L,Pedone EM,Pellecchia M,Leone M

更新日期：2012-03-13 00:00:00

abstract：:Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...

journal_title：Biochemistry

authors： Hübner G,König S,Koch MH,Hengstenberg W

更新日期：1995-12-05 00:00:00

abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...

journal_title：Biochemistry

authors： Darling TL,Fahnestock M

更新日期：1988-09-06 00:00:00