Abstract:
:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting in the formation of heteropolymers (Matsuka, Y. V., Anderson, E. T., Milner-Fish, T., Ooi, P., and Baker, S. (2003) Staphylococcus aureus fibronectin-binding protein serves as a substrate for coagulation factor XIIIa: Evidence for factor XIIIa-catalyzed covalent cross-linking to fibronectin and fibrin, Biochemistry 42, 14643-14652). Factor XIIIa also catalyzes the incorporation in FnbA of fluorescent probes dansylcadaverine and glutamine-containing synthetic peptide patterned on the NH(2)-terminal segment of fibronectin. In this study, the above probes were utilized for site-specific labeling and identification of reactive Gln and Lys residues targeted by factor XIIIa in rFnbA. Probe-decorated rFnbA samples were subjected to trypsin or Glu-C digestion, followed by separation of labeled peptides using reversed phase HPLC. Sequencing and mass spectral analyses of isolated probe-modified peptides have been employed for the identification of factor XIIIa-reactive Gln and Lys residues. Analysis of dansylcadaverine-labeled peptides resulted in the identification of one major, Gln103, and three minor, Gln105, Gln783, and Gln830, amine acceptor sites. The labeling procedure with dansyl-PGGQQIV probe revealed that Lys157, Lys503, Lys620, and Lys762 serve as amine donor sites. The identified reactive glutamine acceptor and lysine donor sites of FnbA may participate in transglutaminase-mediated cross-linking reactions resulting in the covalent attachment of pathogenic Staphylococcus aureus to human host proteins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Anderson ET,Fletcher L,Severin A,Murphy E,Baker SM,Matsuka YVdoi
10.1021/bi049278ksubject
Has Abstractpub_date
2004-09-21 00:00:00pages
11842-52issue
37eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Shiga toxin is a bacterial protein composed of one A and five B subunits. Its A chain possesses a protease sensitive loop (Cys-242-Cys-261) that is cleaved to produce an enzymatically active A1 domain and an A2 fragment associated with its B subunit pentamer. The proposed mode of action of the toxin is linked to its r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960177z
更新日期:1996-07-23 00:00:00
abstract::We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9918994
更新日期:1999-12-14 00:00:00
abstract::The complete amino acid sequence was determined for bovine ubiquitin, and adenylate cyclase stimulating polypeptide, which is probably represented universally in living cells. Ubiquitin has a molecular weight of 8451 and consists of a single polypeptide chain containing 74 amino acid residues. It contains four argini...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00681a026
更新日期:1975-05-20 00:00:00
abstract::The sialic acid binding lectin, limulin, was isolated by gel filtration and ion-exchange chromatography from the hemolymph of Limulus polyphemus. When the purified protein was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol, two major protein bands wer...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00638a026
更新日期:1977-09-20 00:00:00
abstract::In the present study we establish that specific members of the Sp1 family of transcription factors (Sp1 and Sp3) bind to all six GC-rich motifs (elements 1-6) present in the proximal promoter of the human cell cycle inhibitor p21(WAF-1/Cip1) gene. Competition analysis showed that Sp1 and Sp3 bound with high affinity t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026141q
更新日期:2002-10-22 00:00:00
abstract::The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a019
更新日期:1993-03-02 00:00:00
abstract::The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000855o
更新日期:2000-09-05 00:00:00
abstract::A new strategy to form stable and sequence-specific triple helical DNA structures at mixed purine/pyrimidine sequences using a combination of four C-glycosides (TRIPsides) has been described [Li et al. (2003) J. Am. Chem. Soc. 125, 2084]. The partial realization of the approach is demonstrated by incorporating two of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035648d
更新日期:2004-02-17 00:00:00
abstract::Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox).sarcosine) that exhibits an intense ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0600852
更新日期:2006-05-16 00:00:00
abstract::In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9528412
更新日期:1996-04-09 00:00:00
abstract::By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a020
更新日期:1988-05-31 00:00:00
abstract::Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971045o
更新日期:1997-11-04 00:00:00
abstract::Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200892a
更新日期:2011-08-23 00:00:00
abstract::The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00939
更新日期:2019-04-30 00:00:00
abstract::In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00127a030
更新日期:1992-03-31 00:00:00
abstract::The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00050
更新日期:2016-05-10 00:00:00
abstract::The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi6024947
更新日期:2007-05-29 00:00:00
abstract::Translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. Considerable evidence indicates that it plays regulatory roles in steroidogenesis and apoptosis and is involved in various human disea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00142
更新日期:2016-05-24 00:00:00
abstract::Multiple intracellular receptors of the FK506 binding protein (FKBP) family of peptidylprolyl cis/trans-isomerases are potential targets for the immunosuppressive drug FK506. Inhibition of the protein phosphatase calcineurin (CaN), which has been implicated in the FK506-mediated blockade of T cell proliferation, was s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061616p
更新日期:2006-12-26 00:00:00
abstract::Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00063a005
更新日期:1993-03-30 00:00:00
abstract::Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00230a031
更新日期:1991-04-23 00:00:00
abstract::Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00266a042
更新日期:1982-11-09 00:00:00
abstract::Directed hydroxyl radical probing of 16S ribosomal RNA from Fe(II) tethered to specific sites within the RNA was used to determine RNA-RNA proximities in 70S ribosomes. We have transcribed 16S ribosomal RNA in vitro as two separate fragments, covalently attached an Fe(II) probe to a 5'-guanosine-alpha-phosphorothioate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981644a
更新日期:1999-01-19 00:00:00
abstract::The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00394a030
更新日期:1987-10-06 00:00:00
abstract::B₁₂-dependent enzymes employ radical species with exceptional prowess to catalyze some of the most chemically challenging, thermodynamically unfavorable reactions. However, dealing with highly reactive intermediates is an extremely demanding task, requiring sophisticated control strategies to prevent unwanted side rea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500050k
更新日期:2014-06-17 00:00:00
abstract::The HpxO enzyme from Klebsiella pneumoniae was recently proposed, on the basis of genetic studies, to catalyze the hydroxylation of uric acid to 5-hydroxyisourate as part of the purine catabolic pathway. Its primary sequence suggests that the HpxO catalytic activity depends on a flavin cofactor (FAD), contrasting with...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900160b
更新日期:2009-04-14 00:00:00
abstract::Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971707+
更新日期:1998-04-14 00:00:00
abstract::Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962274y
更新日期:1997-04-08 00:00:00
abstract::A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a043
更新日期:1989-08-22 00:00:00
abstract::The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00309a013
更新日期:1984-07-03 00:00:00