Production and characterization of monoclonal antibodies against the two subunits proteins B1 and B2 of Escherichia coli ribonucleotide reductase.

abstract：:The promoter fragment -1550/+129 of the human hepatic triglyceride lipase (HTGL) gene drives the expression of the CAT gene in HepG2 cells, albeit at very low levels. Transient transfections in HepG2 and HeLa cells of 5' deletion constructs indicated that the regulatory elements that control this expression are locate...

journal_title：Biochemistry

authors： Hadzopoulou-Cladaras M,Cardot P

更新日期：1993-09-21 00:00:00

abstract：:The experiments described here explore the role of local sequence in the folding of cellular retinoic acid binding protein I (CRABP I). This is a 136-residue, 10-stranded, antiparallel beta-barrel protein with seven beta-hairpins and is a member of the intracellular lipid binding protein (iLBP) family. The relative ro...

journal_title：Biochemistry

authors： Rotondi KS,Gierasch LM

更新日期：2003-07-08 00:00:00

abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...

journal_title：Biochemistry

authors： Glatz P,Chance B,Vanderkooi JM

更新日期：1979-08-07 00:00:00

abstract：:The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...

journal_title：Biochemistry

authors： Li XM,Momsen MM,Smaby JM,Brockman HL,Brown RE

更新日期：2001-05-22 00:00:00

abstract：:3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...

journal_title：Biochemistry

authors： Wu G,Raines DE,Miller KW

更新日期：1994-12-27 00:00:00

abstract：:Copper thiosemicarbazones cause considerable oxidative stress. This effect may be related to their cytotoxicity. In the present work, the chemical and cellular properties of a new ligand, pyridoxal thiosemicarbazone (H2T), and its copper(II) chelate (CuT) are assessed. CuT is toxic to cultured Ehrlich ascites tumor ce...

journal_title：Biochemistry

authors： Byrnes RW,Mohan M,Antholine WE,Xu RX,Petering DH

更新日期：1990-07-31 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or Arg; the effects on melibiose transport and binding o...

journal_title：Biochemistry

authors： Guan L,Jakkula SV,Hodkoff AA,Su Y

更新日期：2012-04-03 00:00:00

abstract：:During fertilization, free-swimming mouse sperm bind to mZP3 (approximately 83 000 Mr), one of three zona pellucida glycoproteins, and once bound undergo the acrosome reaction, a type of cellular exocytosis [Wassarman, P. M., & Litscher, E. S. (1995) Curr. Top. Dev. Biol. 30, 1-19]. Sperm recognize and bind to specifi...

journal_title：Biochemistry

authors： Litscher ES,Wassarman PM

更新日期：1996-04-02 00:00:00

abstract：:The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydisper...

journal_title：Biochemistry

authors： Linhardt RJ,Merchant ZM,Rice KG,Kim YS,Fitzgerald GL,Grant AC,Langer R

更新日期：1985-12-17 00:00:00

abstract：:The [2Fe-2S] cluster of the Rieske iron-sulfur protein is held between two loops of the protein that are connected by a disulfide bridge. We have replaced the two cysteines that form the disulfide bridge in the Rieske protein of Saccharomyces cerevisiae with tyrosine and leucine, and tyrosine and valine, to evaluate t...

journal_title：Biochemistry

authors： Merbitz-Zahradnik T,Zwicker K,Nett JH,Link TA,Trumpower BL

更新日期：2003-11-25 00:00:00

abstract：:Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appea...

journal_title：Biochemistry

authors： Silow M,Tan YJ,Fersht AR,Oliveberg M

更新日期：1999-10-05 00:00:00

abstract：:hOgg1 and FPG are the primary DNA repair enzymes responsible for removing the major guanine (G) oxidative product, namely, 7,8-dihydro-8-oxoguanine (OG), in humans and bacteria, respectively. While natural G adopts the anti conformation and forms a Watson-Crick pair with cytosine (C), OG can also adopt the syn conform...

journal_title：Biochemistry

authors： Sowlati-Hashjin S,Wetmore SD

更新日期：2018-02-20 00:00:00

abstract：:Apolipoprotein E is immunochemically localized to amyloid plaque in Alzheimer's brains, and the allelic distribution of ApoE in individuals is associated with a disposition toward Alzheimer's disease. We show here that all three ApoE isotypes exhibit a strong and specific ability to inhibit both nucleation and seeding...

journal_title：Biochemistry

authors： Wood SJ,Chan W,Wetzel R

更新日期：1996-09-24 00:00:00

abstract：:Beta-lactams exert their antibiotic action through their inhibition of bacterial DD-peptidases (penicillin-binding proteins). Bacteria, in general, carry several such enzymes localized on the outside of their cell membrane to catalyze the final step in cell wall (peptidoglycan) synthesis. They have been classified int...

journal_title：Biochemistry

authors： Josephine HR,Charlier P,Davies C,Nicholas RA,Pratt RF

更新日期：2006-12-26 00:00:00

abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...

journal_title：Biochemistry

authors： Baek KJ,Das T,Gray CD,Desai S,Hwang KC,Gacchui R,Ludwig M,Im MJ

更新日期：1996-02-27 00:00:00

abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

journal_title：Biochemistry

authors： Wu GY,Wu CH

更新日期：1988-02-09 00:00:00

abstract：:Reductive dehalogenation such as that catalyzed by iodotyrosine deiodinase (IYD) is highly unusual in aerobic organisms but necessary for iodide salvage from iodotyrosine generated during thyroxine biosynthesis. Equally unusual is the dependence of this process on flavin. Rapid kinetics have now been used to define th...

journal_title：Biochemistry

authors： Bobyk KD,Ballou DP,Rokita SE

更新日期：2015-07-28 00:00:00

abstract：:1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measur...

journal_title：Biochemistry

authors： Atkins AR,Martin RC,Smith R

更新日期：1995-02-14 00:00:00

abstract：:We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...

journal_title：Biochemistry

authors： Lakowicz JR,Laczko G,Gryczynski I

更新日期：1987-01-13 00:00:00

abstract：:One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...

journal_title：Biochemistry

authors： Pickhardt M,Larbig G,Khlistunova I,Coksezen A,Meyer B,Mandelkow EM,Schmidt B,Mandelkow E

更新日期：2007-09-04 00:00:00

abstract：:The C-terminus of interferon-gamma (IFNgamma) contains a nuclear localization sequence (NLS) required for the activation and nuclear translocation of the transcription factor STAT1alpha and induction of IFNgamma-activated genes. On the basis of this and other studies, we developed a peptide mimetic of IFNgamma that po...

journal_title：Biochemistry

authors： Subramaniam PS,Flowers LO,Haider SM,Johnson HM

更新日期：2004-05-11 00:00:00

abstract：:The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a n...

journal_title：Biochemistry

authors： Scoville CD,Abraham GN,Turner DH

更新日期：1979-06-12 00:00:00

abstract：:The aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii from Enterococcus faecium is an important microbial resistance determinant and a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. We report here the further characterization of this enzyme in terms of the kinetic mechanism of acetyl transfer and id...

journal_title：Biochemistry

authors： Draker KA,Northrop DB,Wright GD

更新日期：2003-06-03 00:00:00

abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...

journal_title：Biochemistry

authors： Yeagle PL,Alderfer JL,Albert AD

更新日期：1995-11-14 00:00:00

abstract：:By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...

journal_title：Biochemistry

authors： Le Moual H,Quang T,Koshland DE Jr

更新日期：1998-10-20 00:00:00

abstract：:Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...

journal_title：Biochemistry

authors： Ishikawa K,Nakatani H,Katsuya Y,Fukazawa C

更新日期：2007-01-23 00:00:00

abstract：:Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...

journal_title：Biochemistry

authors： Li Z,Pal B,Takenaka S,Tsuyama S,Kitagawa T

更新日期：2005-01-25 00:00:00

abstract：:Standard preparations of Escherichia coli RNA polymerase harbor a 70 kDa protein with NTPase (beta-gamma cleavage) activity that is not a recognized polymerase subunit. The NTPase activity of this component, before and after separation from the polymerase, is strongly dependent on the presence of DNA; single-stranded ...

journal_title：Biochemistry

authors： Butzow JJ,Garland C,Van Lee L,Eichhorn GL

更新日期：1997-12-02 00:00:00

abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...

journal_title：Biochemistry

authors： Stoddard BL,Dean A,Koshland DE Jr

更新日期：1993-09-14 00:00:00