当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Characterization of mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro.

Characterization of mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro.

Abstract：

:During fertilization, free-swimming mouse sperm bind to mZP3 (approximately 83 000 Mr), one of three zona pellucida glycoproteins, and once bound undergo the acrosome reaction, a type of cellular exocytosis [Wassarman, P. M., & Litscher, E. S. (1995) Curr. Top. Dev. Biol. 30, 1-19]. Sperm recognize and bind to specific serine/threonine-linked oligosaccharides located at the mZP3 combining site for sperm. Here, we examined certain characteristics of gp55, a approximately 55 000 Mr glycopeptide derived from the carboxy-terminal half of mZP3 polypeptide to which sperm bind [Rosiere, T. K., & Wassarman, P. M. (1992) Dev. Biol. 154, 309-317]. gp55 is heterogeneous with respect to Mr (approximately 47 000-62 000 Mr) and has a relatively low pI (approximately 4.3-4.5) compared to the polypeptide portion of the glycopeptide (pI approximately 6.5). gp55 inhibits binding of sperm to eggs (i.e., exhibits sperm receptor activity) and induces sperm to undergo the acrosome reaction in vitro at about the same concentrations required for intact mZP3 (approximately 50-200 nM). Each of three different size-fractions of gp55, separated by SDS-PAGE, also exhibits bioactivity in vitro. Removal of asparagine-linked (N-linked) oligosaccharides from gp55, by extensive digestion with N-glycanase, reduces its Mr to approximately 21 000 and increases it pI to approximately 5.3, but does not significantly affect its ability to inhibit binding of sperm to eggs or to induce sperm to undergo the acrosome reaction. Similarly, digestion of gp55 with either endo-beta-galactosidase or neuraminidase alters its Mr and/or pI, but does not significantly affect either of its bioactivities. These observations are consistent with the proposal that neither N-linked oligosaccharides nor sialic acid is an essential element of the mZP3 combining site for sperm. They also indicate that a relatively small mZP3 glycopeptide is able to induce sperm to undergo the acrosome reaction (i.e., cellular exocytosis) in vitro.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Litscher ES,Wassarman PM

doi

10.1021/bi952722m

subject

Has Abstract

pub_date

1996-04-02 00:00:00

pages

3980-5

issue

eissn

0006-2960

issn

1520-4995

pii

bi952722m

journal_volume

pub_type

杂志文章

在线工具

Characterization of mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro.