Urea-induced unfolding of Na,K-ATPase as evaluated by electron paramagnetic resonance spectroscopy.

abstract：:To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...

journal_title：Biochemistry

authors： Hausmann NZ,Znosko BM

更新日期：2012-07-03 00:00:00

abstract：:The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

journal_title：Biochemistry

authors： Sengupta K,Rao BJ

更新日期：2002-12-24 00:00:00

abstract：:Intact rat liver mitochondria were treated with compounds derived from the reaction of fluorescamine with various types of primary amines, including the mycosamine-containing antibiotics amphotericin B and nystatin. The effect of varying amounts of these compounds on ATPase-linked inorganic phosphate (Pi) formation on...

journal_title：Biochemistry

authors： Ramirez F,Shiuan D,Tu SI,Marecek JF

更新日期：1980-04-29 00:00:00

abstract：:The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...

journal_title：Biochemistry

authors： Clore GM,Driscoll PC,Wingfield PT,Gronenborn AM

更新日期：1990-08-14 00:00:00

abstract：:The positions of single amino acids in the interhelical loop regions and the C-terminal tail of bacteriorhodopsin (bR) were investigated by X-ray diffraction using site-directed heavy-atom labeling. Since wild-type bR does not contain any cysteines, appropriate cysteine mutants were produced with a unique sulfhydryl g...

journal_title：Biochemistry

authors： Behrens W,Alexiev U,Mollaaghababa R,Khorana HG,Heyn MP

更新日期：1998-07-21 00:00:00

abstract：:New analogues of 7-methylguanosine 5'-monophosphate (m7GMP) were synthesized with modified 5'-phosphate moieties by replacement of -O with -H, -CH3, or -NH2. Additional analogues were synthesized with 8-methyl- or 8-aminoguanine base substitutions or ring-opened ribose (2',3'-diol). These compounds were analyzed by 1H...

journal_title：Biochemistry

authors： Darzynkiewicz E,Ekiel I,Lassota P,Tahara SM

更新日期：1987-07-14 00:00:00

abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...

journal_title：Biochemistry

authors： Orban-Glaß I,Voskoboynikova N,Busch KB,Klose D,Rickert C,Mosslehy W,Roder F,Wilkens V,Piehler J,Engelhard M,Steinhoff HJ,Klare JP

更新日期：2015-01-20 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...

journal_title：Biochemistry

authors： Kahn TR,Fong KK,Jordan B,Lek JC,Levitan R,Mitchell PS,Wood C,Hatcher ME

更新日期：2009-02-17 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a plasmid-encoded, type II enzyme. Four monomers (78 amino acids long) assemble into a homotetramer possessing 222 symmetry. In previous studies, a tandem array of four R67 DHFR gene copies was fused in frame to generate a functional monomer named Quad1. This protein possessed the...

journal_title：Biochemistry

authors： Feng J,Grubbs J,Dave A,Goswami S,Horner CG,Howell EE

更新日期：2010-08-31 00:00:00

abstract：:The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...

journal_title：Biochemistry

authors： Mendz GL,Brown LR,Martenson RE

更新日期：1990-03-06 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00

abstract：:Atomic force microscopy (AFM) is a technique widely used to image protein-DNA complexes, and its application has now been extended to the measurements of protein-DNA binding constants and specificities. However, the spreading of the protein-DNA complexes on a flat substrate, generally mica, is required prior to AFM im...

journal_title：Biochemistry

authors： Sorel I,Piétrement O,Hamon L,Baconnais S,Cam EL,Pastré D

更新日期：2006-12-12 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...

journal_title：Biochemistry

authors： Merkle D,Block WD,Yu Y,Lees-Miller SP,Cramb DT

更新日期：2006-04-04 00:00:00

abstract：:Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic aci...

journal_title：Biochemistry

authors： Holt JC,Lowey S

更新日期：1975-10-21 00:00:00

abstract：:Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five importa...

journal_title：Biochemistry

authors： Stein RL,Strimpler AM,Hori H,Powers JC

更新日期：1987-03-10 00:00:00

abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...

journal_title：Biochemistry

authors： Ebneth A,Schweers O,Thole H,Fagin U,Urbanke C,Maass G,Wolfes H

更新日期：1994-12-06 00:00:00

abstract：:Biological actions of insulin are initiated by activation of the insulin receptor tyrosine kinase. Protein tyrosine phosphatases (PTPases) PTP1B and PTPalpha are known to dephosphorylate the insulin receptor and may contribute to insulin resistance in diseases such as diabetes. We previously reported that overexpressi...

journal_title：Biochemistry

authors： Chen H,Cong LN,Li Y,Yao ZJ,Wu L,Zhang ZY,Burke TR Jr,Quon MJ

更新日期：1999-01-05 00:00:00

abstract：:An improved purification procedure for the alpha 2 beta 2 complex of tryptophan synthase from Escherichia coli has been developed. It consists of DEAE-Sephacel chromatography, followed by hydrophobic chromatography on Sepharose CL 4B, and leads to material with a higher specific activity than reported previously. Inhi...

journal_title：Biochemistry

authors： Tschopp J,Kirschner K

更新日期：1980-09-16 00:00:00

abstract：:Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...

journal_title：Biochemistry

authors： Stevens AJ,Guan L,Bebenek K,Kunkel TA,Greenberg MM

更新日期：2013-02-05 00:00:00

abstract：:Human granulocyte chemotactic protein 2 (GCP-2) has originally been isolated from cytokine-stimulated osteosarcoma cells as a chemokine coproduced in minute amounts together with interleukin 8. Human GCP-2 (75 residues) was synthesized on a 0.25-mmol scale using Fmoc chemistry. After disulfide bridge formation and pur...

journal_title：Biochemistry

authors： Wuyts A,Van Osselaer N,Haelens A,Samson I,Herdewijn P,Ben-Baruch A,Oppenheim JJ,Proost P,Van Damme J

更新日期：1997-03-04 00:00:00

abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...

journal_title：Biochemistry

authors： Grammel N,Schwartz D,Wohlleben W,Keller U

更新日期：1998-02-10 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...

journal_title：Biochemistry

authors： Bansal A,Karanth NM,Demeler B,Schindelin H,Sarma SP

更新日期：2019-04-16 00:00:00

abstract：:A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

journal_title：Biochemistry

authors： Rad B,Kowalczykowski SC

更新日期：2012-04-03 00:00:00

abstract：:Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...

journal_title：Biochemistry

authors： Lowe PN,Beechey RB

更新日期：1982-08-17 00:00:00

abstract：:Escherichia coli (A)BC excinuclease is the major enzyme responsible for removing bulky adducts, such as pyrimidine dimers and 6-4 photoproducts, from DNA. Mutants deficient in this enzyme are extremely sensitive to UV and UV-mimetic agents, but not to oxidizing agents, or ionizing radiation which damages DNA in part b...

journal_title：Biochemistry

authors： Lin JJ,Sancar A

更新日期：1989-10-03 00:00:00

abstract：:Vaccinia DNA topoisomerase, a 314 amino acid type I enzyme, catalyzes the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward arrow. To identify amino acids that participate in the DNA binding and transesterification st...

journal_title：Biochemistry

authors： Wang LK,Wittschieben J,Shuman S

更新日期：1997-07-01 00:00:00

abstract：:By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...

journal_title：Biochemistry

authors： Peterson S,Ahrling KA,Högblom JE,Styring S

更新日期：2003-03-11 00:00:00

abstract：:In eukaryotes, a major route for ATP-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. In rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabol...

journal_title：Biochemistry

authors： Vierstra RD,Sullivan ML

更新日期：1988-05-03 00:00:00

abstract：:The concentration of hyaluronic acid, chondroitin sulfate, and heparan sulfate was measured in rat brain at 2-day intervals from birth to 1 month of age, and in 40-day-old and adult animals. The levels of all three glycosaminoglycans increased after birth to reach a peak at 7 days after which they declined steadily, a...

journal_title：Biochemistry

authors： Margolis RU,Margolis RK,Chang LB,Preti C

更新日期：1975-01-14 00:00:00