Abstract:
:Intact rat liver mitochondria were treated with compounds derived from the reaction of fluorescamine with various types of primary amines, including the mycosamine-containing antibiotics amphotericin B and nystatin. The effect of varying amounts of these compounds on ATPase-linked inorganic phosphate (Pi) formation on oxygen consumption, and on MgATP-linked and succinate-linked proton movements was examined. The antibiotic-fluorescamine compounds did not affect the Pi formation rate but strongly inhibited both the ATPase-linked and the succinate-linked H+ extrusion rates to approximately the same extent. The antibiotic derivatives decreased the oxygen consumption rate, but this effect was much smaller than the decrease in the respiration-dependent proton extrusion rate. The benzylamine-fluorescamine compound significantly increased the Pi formation rate, in contrast to the antibiotic analogues. The benzylamine derivative, like the antibiotic derivatives, inhibited both types of proton extrusion rates. The slight decrease in the oxygen consumption rate caused by the benzylamine derivative was significantly smaller than the corresponding decrease observed with the antibiotic derivatives. These studies, in which fluorescamine derivatives bind reversibly to mitochondria, are compared with previous studies in which fluorescamine itself binds irreversibly to mitochondria and results in a Pi formation rate increase and MgATP- and succinate-linked proton extrusion rate inhibition but has no effect on the oxygen consumption rate.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ramirez F,Shiuan D,Tu SI,Marecek JFdoi
10.1021/bi00550a031subject
Has Abstractpub_date
1980-04-29 00:00:00pages
1928-33issue
9eissn
0006-2960issn
1520-4995journal_volume
19pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::In order to clarify the functional and structural role of intra- and intersubunit hydrogen bonds in human hemoglobin (Hb A), we prepared two artificial beta chain mutant hemoglobins by site-directed mutagenesis. The mutant Hb Phe-37 beta, in which Trp-37 beta is replaced by Phe to remove the intersubunit hydrogen bond...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00127a030
更新日期:1992-03-31 00:00:00
abstract::The occurrence of isozymes in plant peroxidases is poorly understood. Turnip roots contain seven season-dependent isoperoxidases with distinct physicochemical properties. In the work presented here, multifrequency electron paramagnetic resonance spectroscopy has been used to characterize the Compound I intermediate ob...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002826j
更新日期:2001-06-12 00:00:00
abstract::Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952233m
更新日期:1996-04-30 00:00:00
abstract::The prokaryotic DNA protection during starvation (Dps) proteins typically protect macromolecules against damaging agents via physical association with DNA and by oxidizing and sequestering iron. However, Deinococcus radiodurans Dps-1, which binds DNA with high affinity, fails to protect DNA against hydroxyl radicals d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300703x
更新日期:2012-08-21 00:00:00
abstract::We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951314f
更新日期:1996-03-05 00:00:00
abstract::Electron paramagnetic resonance (EPR) measurements were performed on photosystem II (PSII) membranes that were treated with 2 M NaCl to release the 17- and 23-kilodalton (kDa) polypeptides. By using 75 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea to limit the photosystem II samples to one stable charge separation in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00369a022
更新日期:1986-10-21 00:00:00
abstract::The two key structural features of alpha 2-macroglobulin (alpha 2M) involved in inhibitory caging of proteases are the thiol ester and the bait region. This paper examines the environment of the hydrolyzed thiol ester in methylamine-treated human alpha 2M and the separation between the bait region and the thiol ester ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00408a036
更新日期:1988-04-19 00:00:00
abstract::Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200644c
更新日期:2011-09-27 00:00:00
abstract::It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a052
更新日期:1994-08-16 00:00:00
abstract::Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061632m
更新日期:2006-12-26 00:00:00
abstract::Fluid-phase interactions between hematologic cells and those of the vessel wall were studied in order to define a role for lipoxygenase products as cell signals in the control of vascular cholesterol metabolism. A functional parameter for hydroxy acids in this system has not been previously demonstrated. We report her...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00448a030
更新日期:1989-10-31 00:00:00
abstract::Quinone oxidoreductase 2 (QR2) purified from human red blood cells was recently shown to be a potential target of the quinoline antimalarial compounds [Graves et al., (2002) Mol. Pharmacol. 62, 1364]. QR2 catalyzes the two-electron reduction of menadione via the oxidation of N-alkylated or N-ribosylated nicotinamides....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035923w
更新日期:2004-04-20 00:00:00
abstract::The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00852
更新日期:2020-01-14 00:00:00
abstract::Thiol dioxygenases are mononuclear non-heme FeII-dependent metalloenzymes that initiate the oxidative catabolism of thiol-containing substrates to their respective sulfinates. Cysteine dioxygenase (CDO), the best characterized mammalian thiol dioxygenase, contains a three-histidine (3-His) coordination environment rat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00267
更新日期:2020-07-07 00:00:00
abstract::The fluorescence characteristics of brevin and the effects of Ca2+ on the protein conformation were fully investigated. Brevin contains 18 tryptophans and 27 tyrosines. Analysis of the fluorescence spectra and the accessibility to quenching molecules indicate that the emitting tryptophans are located in a hydrophobic ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a055
更新日期:1985-09-24 00:00:00
abstract::The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961985t
更新日期:1996-12-03 00:00:00
abstract::A receptor binding region of mouse interferon gamma (IFN gamma) has previously been localized to the N-terminal 39 amino acids of the molecule by use of synthetic peptides and monoclonal antibodies. In this report, a detailed analysis of the synthetic peptide corresponding to this region, IFN gamma (1-39), is presente...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00237a022
更新日期:1991-06-11 00:00:00
abstract::RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200837b
更新日期:2011-09-20 00:00:00
abstract::Oxalate:ferredoxin oxidoreductase (OOR) is an unusual member of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid:ferredoxin oxidoreductase (OFOR) family in that it catalyzes the coenzyme A (CoA)-independent conversion of oxalate into 2 equivalents of carbon dioxide. This reaction is surprising because binding of C...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00222
更新日期:2017-06-06 00:00:00
abstract::Several 3-oxo-4-aza steroids (1) have been identified as inhibitors of the 3 beta-hydroxy-delta 5-steroid dehydrogenase/3-keto-delta 5-steroid isomerase catalyzed conversion of pregnenolone to progesterone. By kinetically decoupling the two enzyme activities isolated from bovine adrenal cortex, it has been demonstrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a021
更新日期:1989-01-10 00:00:00
abstract::Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0347104
更新日期:2004-02-17 00:00:00
abstract::Dipeptide substrates for metalloendoproteases have previously been shown to block biological processes requiring membrane fusion. Thus, we employed such compounds as potential inhibitors of the insulin-dependent activation of glucose transport in fat cells. This event is thought to involve vesicle movement from an int...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00361a031
更新日期:1986-07-01 00:00:00
abstract::The preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid I, are described. CO association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00305a011
更新日期:1984-05-08 00:00:00
abstract::The C-terminus of interferon-gamma (IFNgamma) contains a nuclear localization sequence (NLS) required for the activation and nuclear translocation of the transcription factor STAT1alpha and induction of IFNgamma-activated genes. On the basis of this and other studies, we developed a peptide mimetic of IFNgamma that po...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036213t
更新日期:2004-05-11 00:00:00
abstract::Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00012a002
更新日期:1995-03-28 00:00:00
abstract::Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a031
更新日期:1989-05-30 00:00:00
abstract::Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060502a
更新日期:2006-05-23 00:00:00
abstract::A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00614
更新日期:2020-09-29 00:00:00
abstract::The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00684a011
更新日期:1975-07-01 00:00:00
abstract::Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00051a015
更新日期:1995-12-26 00:00:00